1997
DOI: 10.1021/bi9711745
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Multiple RRMs Contribute to RNA Binding Specificity and Affinity for Polypyrimidine Tract Binding Protein

Abstract: Polypyrimidine tract binding protein (PTB) is a 57 kD hnRNP protein (hnRNP I) that binds to the pyrimidine tract typically found near the 3' end of introns. Primary sequence analysis suggests that PTB contains four RNA recognition motifs (RRMs). Data from comparative structural and deletional analysis of PTB are consistent with the presence of a four reiterated domain structure. Since PTB exists in solution as a homodimer, it contains an oligomeric array of eight RRMs. Though the function of RRMs in a monomeri… Show more

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Cited by 162 publications
(198 citation statements)
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“…PTB is thought to be a nuclear protein in the static state. It can shuttle between the nucleus and cytoplasm, because the RRM 2 domain of PTB facilitates the nuclear export of PTB, whereas RRM 1, 3, and 4 may contribute to the nuclear localization of PTB [46][47][48]. PTB is also an RNA-binding protein; indeed, PTB has been shown to bind to both 5¢UTR [21] …”
Section: Discussionmentioning
confidence: 99%
“…PTB is thought to be a nuclear protein in the static state. It can shuttle between the nucleus and cytoplasm, because the RRM 2 domain of PTB facilitates the nuclear export of PTB, whereas RRM 1, 3, and 4 may contribute to the nuclear localization of PTB [46][47][48]. PTB is also an RNA-binding protein; indeed, PTB has been shown to bind to both 5¢UTR [21] …”
Section: Discussionmentioning
confidence: 99%
“…49). PTB is known to be a homodimer that belongs to a family of RNA-binding proteins characterized by possession of at least one RNA recognition motif (54) and was originally identified as a nuclear-localized splicing factor that binds to pyrimidine-rich tracts within pre-mRNAs of a large number of genes (Refs. 46, 47, and 55; reviewed in Ref.…”
Section: Discussionmentioning
confidence: 99%
“…Similar results were obtained in three independent experiments prepared and transduced a short form of both PTB1 and PTB4 starting at Met175. Short PTB lacked the N-terminal nuclear localization signal (NLS) and the first RRM, which has been shown to influence nuclear shuttling 21,22 and to be dispensable for RNA binding. 21 Unexpectedly, although PTB short forms localized to the cytosol, an important pool was still directed to the nucleus (Figure 5a).…”
Section: Resultsmentioning
confidence: 99%
“…Short PTB lacked the N-terminal nuclear localization signal (NLS) and the first RRM, which has been shown to influence nuclear shuttling 21,22 and to be dispensable for RNA binding. 21 Unexpectedly, although PTB short forms localized to the cytosol, an important pool was still directed to the nucleus (Figure 5a). PTB short forms retained the capacity to induce nPTB exon 10 skipping ( Figure 5b) and to enhance expression of Apaf-1 and Caspase-3 but were not efficient to sustain Caspase-9, Bax, and Bid expression ( Figure 5c).…”
Section: Resultsmentioning
confidence: 99%
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