Multiple traces of monkeypox detected in non-sewered wastewater with sparse sampling from a densely populated metropolitan area in Asia

Wannigama, Dhammika Leshan; Amarasiri, Mohan; Hongsing, Parichart; Hurst, Cameron; Modchang, Charin; Chadsuthi, Sudarat; Anupong, Suparinthon; Phattharapornjaroen, Phatthranit; S.M., Ali Hosseini Rad; Fernandez, Stefan; Huang, Angkana T.; Kueakulpattana, Naris; Tanasatitchai, Chanikan; Vatanaprasan, Porames; Saethang, Thammakorn; Luk-in, Sirirat; Storer, Robin James; Ounjai, Puey; Ragupathi, Naveen Kumar Devanga; Kanthawee, Phitsanuruk; Sano, Daisuke; Furukawa, Takashi; Sei, Kazunari; Leelahavanichkul, Asada; Kanjanabuch, Talerngsak; Hirankarn, Nattiya; Higgins, Paul G.; Kicic, Anthony; Chatsuwan, Tanittha; McLellan, Alexander D.; Abe, Shuichi

doi:10.1016/j.scitotenv.2022.159816

Cited by 23 publications

(13 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, our processing method may capture a wide array of microbes, however it certainly misses others. For example, despite relatively high local incidences, we have not been able to detect mpox genomes in our samples in contrast to other sites (Wannigama et al, 2023; Wolfe et al, 2022b). Also, temperature differences (in Berlin, wastewater is in the range between 15 °C in winter and 25 °C) as well as the flow time from the households to the treatment plant (in the range of one to several hours) can introduce variability that is difficult to track.…”

Section: Discussioncontrasting

confidence: 71%

Comprehensive profiling of wastewater viromes by genomic sequencing

Wyler

Lauber

Manukyan

et al. 2022

Preprint

View full text Add to dashboard Cite

Genomic material in wastewater provides a rich source of data for detection and surveillance of microbes. Used for decades to monitor poliovirus and other pathogens, the SARS-CoV-2 pandemic and the falling costs of high-throughput sequencing have substantially boosted the interest in and the usage of wastewater monitoring. We have longitudinally collected over 100 samples from a wastewater treatment plant in Berlin/Germany, from March 2021 to July 2022, in order to investigate three aspects. First, we conducted a full metagenomic analysis and exemplified the depth of the data by temporal tracking strains and to a certain extent also variants of human astroviruses and enteroviruses. Second, targeting respiratory pathogens, a broad enrichment panel enabled us to detect waves of RSV, influenza, or common cold coronaviruses in high agreement with clinical data. Third, by applying a profile Hidden Markov Model-based search for novel viruses, we identified more than 100 thousand novel transcript assemblies likely not belonging to known virus species, thus substantially expanding our knowledge of virus diversity. Taken together, we present a longitudinal and deep investigation of the viral genomic information in wastewater that underlines the value of sewage surveillance for both public health purposes and planetary virome research.

show abstract

Section: Discussioncontrasting

confidence: 71%

Comprehensive profiling of wastewater viromes by genomic sequencing

Wyler

Lauber

Manukyan

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…The WHO has relied on naming it the 2022 human MPOX, and we use this term in the study 1 . Multiple studies discussed the 2022 human MPOX status, for example; it is found a detection of traces of the MPOX in wastewater with sparse sampling from a Asian densely populated areas 2 . Also, some studies discussed applying the epidemic models, including the fractional models, and other control models 3 – 5 .…”

Section: Introductionmentioning

confidence: 99%

The study of human monkeypox disease in 2022 using the epidemic models: herd immunity and the basic reproduction number case

Al‐Raeei

2023

Annals of Medicine &Amp; Surgery

View full text Add to dashboard Cite

As of May 2022, a new outbreak of the human monkeypox (MPOX) disease appeared in multiple countries, where the 2022 human MPOX disease spread to more than 109 cases, excluding the suspected cases up to the end of 2022. The deaths of the 2022 human MPOX exceeded 200 cases up to the same date. The human MPOX is not a new disease, this disease was once endemic in some countries on the African continent. Despite this, this disease began to spread in a number of countries around the world in 2022. The first case of the 2022 human MPOX was recorded in the United Kingdom in May. After that date, this disease began to become a pandemic in a number of other countries, such as the United States, Spain, and Brazil. The 2022 human MPOX is a type of viral disease caused by a viral virus, the MPOX virus, and this virus causes rashes and lesions over the skin of the patient, as well as in the mouth of the patient. Multiple effective indicators are employed for the study of the 2022 of the human MPOX, such as the herd immunity of the human MPOX (HIhMPOX), the basic reproduction number of the human MPOX (BRNhMPOX), and the infection period of the human MPOX. This study focuses on the study of the herd immunity of, and the basic reproduction number of the 2022 outbreak of human MPOX in multiple countries around the world. This study employed the semianalytical method of the Susceptible compartment S, Infectious compartment I, Recovered compartment R (SIR) pandemic model including the mortality for the study of the herd immunity, and the basic reproduction number of the 2022 human MPOX disease. It is found that the average value of the herd immunity for the human MPOX disease in 2022 equals to 0.2194, that is, 21.94% for multiple countries, and equals to 35.52% for the United States, and 30.99% for Spain. Also, it is found that the average value of the basic reproduction number of the 2022 human MPOX disease equals to 1.2810 for multiple countries. It is concluded from these values that 21.94% of the total susceptible population has to be immunized in an effective way to prevent the spreading of the disease. Also, based on the previous values, it is concluded that the status of the 2022 MPOX disease is spreading as a pandemic.

show abstract

“…In addition to identifying when to isolate and thereby helping interrupt chains of transmission, 11 , 77 community testing is crucial for identifying the need for therapeutic interventions that prevent disease progression and clinical deterioration, 78 not only for COVID-19 but other infections too. 69 , 79 , 80 In light of the results of this trial, communities that do not encourage regular testing for COVID-19 may be placed at a disadvantage and face an increased healthcare burden compared to communities that encourage testing only at the point where therapeutic intervention is clearly required.…”

Section: Discussionmentioning

confidence: 99%

Early treatment with fluvoxamine, bromhexine, cyproheptadine, and niclosamide to prevent clinical deterioration in patients with symptomatic COVID-19: a randomized clinical trial

Wannigama,

Hurst,

Phattharapornjaroen

et al. 2024

eClinicalMedicine

Self Cite

View full text Add to dashboard Cite

Multiple traces of monkeypox detected in non-sewered wastewater with sparse sampling from a densely populated metropolitan area in Asia

Cited by 23 publications

References 19 publications

Comprehensive profiling of wastewater viromes by genomic sequencing

Comprehensive profiling of wastewater viromes by genomic sequencing

The study of human monkeypox disease in 2022 using the epidemic models: herd immunity and the basic reproduction number case

Early treatment with fluvoxamine, bromhexine, cyproheptadine, and niclosamide to prevent clinical deterioration in patients with symptomatic COVID-19: a randomized clinical trial

Contact Info

Product

Resources

About