Multiple transcriptome analysis of Piwil2-induced cancer stem cells, including piRNAs, mRNAs and miRNAs reveals the mechanism of tumorigenesis and development

Tan, Xiaojun; Mi, Tao; Zhang, Zhaoxia; Jin, Liming; Wang, Zhang; Wu, Xing-Zheng; Wang, Jinkui; Li, Mujie; Zhanghuang, Chenghao; He, Dalin

doi:10.1007/s11033-022-07237-z

Cited by 4 publications

(4 citation statements)

References 33 publications

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“…Studies have shown that PIWIL2 may impact prostate cancer [ 4 ], cervical cancer [ 5 ], oral squamous cell carcinoma [ 6 ], and esophageal squamous cell carcinoma [ 7 ]. In a multiple transcriptome analysis, it was also found that PIWIL2-induced cancer stem cells showed significant alterations in the levels of small RNAs [ 8 ]. Additionally, distinct changes in PIWIL2 expression were observed in thyroid cancer (TC) [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

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PIWIL2 restrains the progression of thyroid cancer via interaction with miR-146a-3p

Lu,

Zhu,

et al. 2023

BMC Endocr Disord

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Objective The classical role of PIWIL2 is to regulate reproduction by binding to piRNA, but its tumor-related function has received increasing attention in recent years. This study aims to explore its role in the progression of thyroid cancer (TC). Methods First, we measured and analyzed the levels of PIWIL2 and miR-146a-3p in TC tissue and adjacent tissues as well as several TC cell lines. We demonstrated the clinical significance of PIWIL2 and miR-146a-3p through the survival rate. Based on these results, we selected TPC-1 and KTC-3 cell lines for our cell experiments. We treated these cell lines with PIWIL2 lentivirus, PIWIL2 siRNA, miR-146a-3p mimic, or miR-146a-3p inhibitor and measured cell proliferation, cell cycle, apoptosis, migration, and invasion. We used PCR and Western blot to quantify the mRNA and protein levels of PIWIL2, while we used luciferase reporter assay and RNA binding protein immunoprecipitation to explore the relationship between miR-146a-3p and PIWIL2. Finally, we developed a xenograft tumor model to confirm the effects of the miR-146a-3p/PIWIL2 axis on TC progression in vivo. Results We identified that PIWIL2 and miR-146a-3p exhibit opposite expression alterations in TC tissues and that PIWIL2 serves as a ‘sponge’ by adsorbing miR-146a-3p. Up-regulating PIWIL2 decelerated the proliferation, metastasis, and cell cycle progression of TPC-1 and KTC-3 cells, but accelerated the apoptosis of TC cells, while miR-146a-3p exhibited opposite effects. Finally, overexpressing PIWIL2 restrained the progression of TC in nude mice, which can be reversed by increasing miR-146a-3p expression. Inhibiting PIWIL2, on the other hand, promoted the progression of TC in vivo, which can be reversed by inhibiting miR-146a-3p. Conclusion PIWIL2 may inhibit the progression of TC by sponging miR-146a-3p, providing new insights into the early treatment, recrudescence treatment, and metastasis treatment of TC.

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Section: Introductionmentioning

confidence: 99%

“…In a multiple transcriptome analysis, it was also found that PIWIL2-induced cancer stem cells showed significant alterations in the levels of small RNAs [ 8 ]. Additionally, distinct changes in PIWIL2 expression were observed in thyroid cancer (TC) [ 8 ]. However, the pathological role of PIWIL2 in TC is yet to be determined.…”

Section: Introductionmentioning

confidence: 99%

PIWIL2 restrains the progression of thyroid cancer via interaction with miR-146a-3p

Lu,

Zhu,

et al. 2023

BMC Endocr Disord

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“…The roles of TMEM184B in the biological processes of cancer are also gaining attention. In a study on the formation mechanism of piWIL2‐induced cancer stem cells (Piwil‐iCSCs) by Tan et al, 27 TMEM184B formed a fusion gene, tMEM184B‐DMC1, with DMC1, and might be related to the occurrence of cancer stem cells. Sara et al 28 found that the single nucleotide polymorphisms (SNP) rs7289126 in TMEM184B were associated with both per cent density (PD) and dense area (DA) in mammograms and the risk of breast cancer.…”

Section: Discussionmentioning

confidence: 99%

TMEM184B promotes proliferation, migration and invasion, and inhibits apoptosis in hypopharyngeal squamous cell carcinoma

Lin

Liu

et al. 2022

J Cellular Molecular Medi

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Hypopharyngeal squamous cell carcinoma (HPSCC) accounts for approximately 3% of all head and neck squamous cell carcinoma (HNSCC). The incidence of HPSCC has increased during the past three decades. 1,2 Although HPSCC is relatively uncommon, it is one of aggressive and lethal forms among HNSCCs. HPSCC is characterized by its wide submucosal spread, early lymph node metastasis, distal metastasis and diagnosis with a later stage. Although improvements have been made to treatments, such as surgery, radiotherapy, chemotherapy, immunotherapy and in their combination in recent decades, 3,4 the 5 years overall survival of HPSCC remains approximately 30%-35%. 5 As hypopharynx is anatomically adjacent to the larynx, the advancedstage HPSCC may severely impair the structure and function of the

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“…According to this hypothesis, cancers arise from a small group of stem cell like cells which are responsible for tumor initiation and growth. One of the articles in this special issue reports the altered expression of several miRNAs, piRNAs and mRNAs in PIWIL2-reprogrammed fibroblast cells (PIWIL2-iCSCs)—lab-designed cancer stem cell-like cells [ 11 ]. PIWIL2 is recognized as a cancer–testis antigen (CTA) and is known to be involved in genesis and/or maintenance of cancer stem cells [ 12 ].…”

Section: Non-coding Rnas and Stem Cellsmentioning

confidence: 99%

Non-coding RNAs: the silent regulators of health and diseases

Chakravorty

2022

Mol Biol Rep

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Non-coding RNAs (ncRNAs) like miRNAs, siRNA, lncRNAs, circRNAs, piRNAs, snoRNAs, snRNAs etc. form a collective group of RNAs that is instrumental to the various functions of the genome. With the advent of cutting-edge molecular biology tools and techniques, scientists have unearthed several mechanisms through which these ncRNAs act. Although our understanding may still be limited, yet scientists have been able to establish ncRNAs as major regulators of genetic inter-plays that dictate various pathophysiological conditions. This special issue of Molecular Biology Reports features a collection of research and review articles on ncRNAs and their involvement in different pathophysiological conditions that include different types of cancers. It is expected that this special issue will motivate researchers in the field to delve deeper into the world of ncRNAs and attempt to develop new diagnostic and therapeutic interventions for challenging clinical conditions.

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Multiple transcriptome analysis of Piwil2-induced cancer stem cells, including piRNAs, mRNAs and miRNAs reveals the mechanism of tumorigenesis and development

Cited by 4 publications

References 33 publications

PIWIL2 restrains the progression of thyroid cancer via interaction with miR-146a-3p

PIWIL2 restrains the progression of thyroid cancer via interaction with miR-146a-3p

TMEM184B promotes proliferation, migration and invasion, and inhibits apoptosis in hypopharyngeal squamous cell carcinoma

Non-coding RNAs: the silent regulators of health and diseases

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