Multiple wavelengths maximization fusion fingerprint profiling for quality evaluation of compound liquorice tablets and related antioxidant activity analysis

Gong, Dandan; Chen, Jinyu; Sun, Yu; Liu, Xitong; Sun, Guoxiang

doi:10.1016/j.microc.2020.105671

Cited by 24 publications

(13 citation statements)

References 25 publications

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“…IC 50 , half inhibitory concentration, is a common index to measure the efficacy of drugs. The smaller the IC 50 value, the stronger the free radical scavenging ability of sample [22,23].…”

Section: Abts * + Cation Radical Scavenging Activity Assaymentioning

confidence: 99%

Multi Wavelengths Fusion Profiling for Quality Evaluation of Donglingcao Tablets Combined with Electrochemical Oscillation Fingerprints and Antioxidant Activity Analysis

Shen

Gong

et al. 2022

Electroanalysis

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For a long time, Donglingcao tablets (DLCT) has been considered as a valuable traditional Chinese medicine (TCM) preparation. The five-wavelength fusion fingerprints (FWFFP) of 16 batches of DLCT, which avoided fluctuation of single wavelength evaluation, were established to analyze fingerprint similarity by ratio fingerprint quantitative method (RFQM). Furthermore, electrochemical oscillation fingerprints (ECOFP) of DLCT from different manufacturers were studied by Belousov Zhabotinskii (B-Z) oscillating system at con-stant temperature. In addition, intrinsic characteristics of DLCT from different manufacturers were determined by chemometric methods, and they can well distinguish samples of each manufacturer. Meanwhile, partial least squares (PLS) model was built to predict antioxidant activity of DLCT and to correlate with components in the fingerprint, which proved significant contribution of peaks 16-rosmarinic acid and 19-oridonin. The combination of FWFFP with antioxidant and ECOFP provided a new idea for identification and analysis of DLCT.

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“…IC 50 , half inhibitory concentration, is a common index to measure the efficacy of drugs. The smaller the IC 50 value, the stronger the free radical scavenging ability of sample [22,23].…”

Section: Abts * + Cation Radical Scavenging Activity Assaymentioning

confidence: 99%

Multi Wavelengths Fusion Profiling for Quality Evaluation of Donglingcao Tablets Combined with Electrochemical Oscillation Fingerprints and Antioxidant Activity Analysis

Shen

Gong

et al. 2022

Electroanalysis

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“…To obtain as many peaks as conceivable while using a straightforward and helpful strategy, three critical parameters controlling extraction yield, including extraction solvent (water; 50/50, 70/30 MeOH/H 2 O (v/v); and EtOH), sample-to-solvent ratio (1-10, 1-25, and 1-50), and ultrasonic extraction time (15,30,45, and 60 min), were studied. e results demonstrated that 1.0 g-25 mL 70% of methanol was the best combination, which yielded more peaks and higher relative peak intensity.…”

Section: Optimization Of the Sample Extraction And Hplc-dad Conditionsmentioning

confidence: 99%

“…Fingerprint and LC-MS technology are internationally recognized means of cutting-edge supervision, and not only conform to the feasible mode of overall control in TCM theory, but also reflect the aspects of cutting-edge explanatory innovation in setting up an international quality standard framework in keeping with TCM's characteristics [ 10 – 13 ]. In addition, LC-MS provides higher sensitivity and selectivity for assaying the components that are difficult to separate effectively or be detected due to low content or lack of chromophore groups by HPLC-DAD [ 14 , 15 ]. During the past few years, chemical pattern recognition has attracted increasing attention within the areas of information handling, which was regarded as a successful strategy for evaluating TCM's batch-to-batch consistency [ 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Quality Evaluation of Traditional Chinese Medicine Prescription in Naolingsu Capsule Based on Combinative Method of Fingerprint, Quantitative Determination, and Chemometrics

Yang²,

Cui³

et al. 2022

Journal of Analytical Methods in Chemistry

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Background. Naolingsu capsule (NLSC) is a well-known traditional Chinese medicine (TCM) prescription in China. It is widely used to treat neurasthenia, insomnia, cardiovascular and cerebrovascular disease, and other diseases. However, its inalienable chemical groups have not been carried out. Methods. We first established the nontargeted investigation based on fingerprinting coupled with UHPLC-Q/TOF-MS/MS. Second, the quantitative methods based on HPLC-DAD and LC-MS/MS were connected to the synchronous quantitative assurance of eleven and fourteen marker compounds. Finally, the quantitative information was processed with SIMCA-P for differentiating the distinctive bunches of samples to screen the foremost appropriate chemical markers. Results. The similarity of HPLC fingerprints of 24 batches of NLSC samples was 0.645–0.992. In total, 37 flavonoids, 21 organic acids, 22 lignans, 13 saponins, and 20 other compounds were recognized in NLSC by the UHPLC-Q/TOF-MS/MS method. The quantitative determination was approved for linearity, discovery limits, accuracy, repeatability, soundness, and precision. Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) models accomplished the great classification of the samples from the five enterprises, respectively. Rehmannioside D (RD), methylophiopogonanone A (MPA), 3,6′-disinapoyl sucrose (DS), schisandrin B (SSB), epimedin C (EC), icariin (ICA), and jujuboside B (JB) were considered as the potential chemical markers for NLSC quality control. Conclusion. The experimental results illustrated that the combinative strategy was valuable for quick pharmaceutical quality assessment, which can potentially differentiate the origin, decide the realness, and assess the overall quality of the formulation.

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“…Time window width was set as 0.1, average mode was used, and then, all the samples had multipoint correction and automatic match to generate a representative fingerprint that represented the characteristic mode. e similarity was calculated by comparing chromatograms of saffron with the representative fingerprints [35,36]. e results showed that the similarity of 21 batches of saffron was 0.962-0.998 at 257 nm and 0.992-1 at 440 nm.…”

Section: Hplc Fingerprint Analysis and Similarity Analysismentioning

confidence: 99%

Multivariate Statistical Analysis Uncovers Spectrum–Effect Relationship between HPLC Fingerprints and Antioxidant Activity of Saffron

You

Zhong

et al. 2021

Journal of Chemistry

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Crocus sativus L. is commonly used as functional food and medicinal herb in traditional Chinese medicine. In this study, the spectrum–effect relationship was established between HPLC fingerprints and in vitro antioxidant activity of saffron to improve the quality evaluation method of saffron. The fingerprints of 21 batches of saffron collected from different regions were assessed, and the data were further analyzed by chemometric methods, including similarity analysis, hierarchical clustering analysis, principal component analysis, and orthogonal partial least squares discriminant analysis. The spectrum–effect relationship between fingerprints and antioxidant effect of saffron was analyzed by grey relational analysis and partial least square methods to figure out the antioxidant component of saffron. Thirteen common peaks of 21 batches of saffron were included in the analysis, and peak 3 (picrocrocin), peak 7 (crocin I), and peak 10 (crocin II) were identified as the main active components responsible for antioxidant efficacy. Besides, a multi-index quality control method was developed for simultaneous determination of these three antioxidant components in saffron. Taken together, this study provided new strategies for the quality control and the development of new bioactive products of saffron in the future.

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Multiple wavelengths maximization fusion fingerprint profiling for quality evaluation of compound liquorice tablets and related antioxidant activity analysis

Cited by 24 publications

References 25 publications

Multi Wavelengths Fusion Profiling for Quality Evaluation of Donglingcao Tablets Combined with Electrochemical Oscillation Fingerprints and Antioxidant Activity Analysis

Multi Wavelengths Fusion Profiling for Quality Evaluation of Donglingcao Tablets Combined with Electrochemical Oscillation Fingerprints and Antioxidant Activity Analysis

Quality Evaluation of Traditional Chinese Medicine Prescription in Naolingsu Capsule Based on Combinative Method of Fingerprint, Quantitative Determination, and Chemometrics

Multivariate Statistical Analysis Uncovers Spectrum–Effect Relationship between HPLC Fingerprints and Antioxidant Activity of Saffron

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