Multiplex bacterial PCR for antibiotic stewardship in pneumonia – Author's reply

Darie, Andrei; Stolz, Daiana

doi:10.1016/s2213-2600(22)00269-7

Cited by 4 publications

(2 citation statements)

References 5 publications

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“…It has been suggested that rapid detection of S. aureus and other bacterial causative agents of CAP would result in an overall reduction in the use of anti-MRSA antibiotics, allowing for better antibiotic stewardship [ 32 ]. Importantly, a recent study suggested that utilization of multiplex bacterial PCR may reduce inappropriate antibiotic use, potentially promoting good antibiotic stewardship in hospitalized patients with bacterial pneumonia [ 34 ]. In the context of antimicrobial stewardship, early detection of infecting pathogens in patients presenting ILI symptoms can also help discriminate between viral, bacterial, and fungal infections, and help determine the appropriate treatment decision.…”

Section: Discussionmentioning

confidence: 99%

Expanded PCR Panel Testing for Identification of Respiratory Pathogens and Coinfections in Influenza-like Illness

Upadhyay¹,

Reddy²,

Proctor

et al. 2023

Diagnostics

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While COVID-19 has dominated Influenza-like illness (ILI) over the past few years, there are many other pathogens responsible for ILI. It is not uncommon to have coinfections with multiple pathogens in patients with ILI. The goal of this study was to identify the different organisms in symptomatic patients presenting with ILI using two different high throughput multiplex real time PCR platforms. Specimens were collected from 381 subjects presenting with ILI symptoms. All samples (nasal and nasopharyngeal swabs) were simultaneously tested on two expanded panel PCR platforms: Applied Biosystems™ TrueMark™ Respiratory Panel 2.0, OpenArray™ plate (OA) (32 viral and bacterial targets); and Applied Biosystems™ TrueMark™ Respiratory Panel 2.0, TaqMan™ Array card (TAC) (41 viral, fungal, and bacterial targets). Results were analyzed for concordance between the platforms and for identification of organisms responsible for the clinical presentation including possible coinfections. Very good agreement was observed between the two PCR platforms with 100% agreement for 12 viral and 3 bacterial pathogens. Of 381 specimens, approximately 58% of the samples showed the presence of at least one organism with an important incidence of co-infections (~36–40% of positive samples tested positive for two and more organisms). S. aureus was the most prevalent detected pathogen (~30%) followed by SARS-CoV-2 (~25%), Rhinovirus (~15%) and HHV6 (~10%). Co-infections between viruses and bacteria were the most common (~69%), followed by viral-viral (~23%) and bacterial-bacterial (~7%) co-infections. These results showed that coinfections are common in RTIs suggesting that syndromic panel based multiplex PCR tests could enable the identification of pathogens contributing to coinfections, help guide patient management thereby improving clinical outcomes and supporting antimicrobial stewardship.

show abstract

Section: Discussionmentioning

confidence: 99%

Expanded PCR Panel Testing for Identification of Respiratory Pathogens and Coinfections in Influenza-like Illness

Upadhyay¹,

Reddy²,

Proctor

et al. 2023

Diagnostics

View full text Add to dashboard Cite

show abstract

“…It has been suggested that rapid detection of S. aureus and other bacterial causative agents of CAP would result in an overall reduction in the use of anti-MRSA antibiotics allowing for better antibiotic stewardship [32]. Importantly, a recent study suggested that utilization of multiplex bacterial PCR may reduce inappropriate antibiotic use, potentially promoting good antibiotic stewardship in hospitalized patients with bacterial pneumonia [34]. In the context of antimicrobial stewardship, early detection of infecting pathogens in patients presenting ILI symptoms can also help discriminate between viral, bacterial and fungal infection and help determine the appropriate treatment decision.…”

Section: Discussionmentioning

confidence: 99%

Expanded PCR Panel Testing for Identification of Respiratory Pathogens and Coinfections in Influenza-like Illness

Upadhyay¹,

Reddy²,

Proctor³

et al. 2023

Preprint

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Respiratory tract infections (RTIs) are one of the main causes of hospitalization and mortality causing substantial economic burden to healthcare systems globally. As opposed to the previous belief that respiratory infections are caused by a single pathogen, studies have shown that most RTIs are a result of a combination of bacterial and/or viral pathogens infecting the host. The clinical manifestation of RTIs is very similar (i.e., syndrome), often showing Influenza-like illness (ILI) symptoms. While COVID-19 has dominated ILI over the past few years, there are many other pathogens that are responsible for ILI. In addition, it is not uncommon to have coinfections with multiple pathogens in patients presenting with ILI and that such coinfections can even exacerbate the disease severity of RTIs. Therefore, an insight into coinfections can help with accurate disease prognosis, patient care management and outcomes. The goal of this study was to identify the different organisms in symptomatic patients presenting with ILI.

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Antibiotic treatment of community-acquired and hospital-acquired pneumonia

Simon

Rademacher

2023

Med Klin Intensivmed Notfmed

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Multiplex bacterial PCR for antibiotic stewardship in pneumonia – Author's reply

Cited by 4 publications

References 5 publications

Expanded PCR Panel Testing for Identification of Respiratory Pathogens and Coinfections in Influenza-like Illness

Expanded PCR Panel Testing for Identification of Respiratory Pathogens and Coinfections in Influenza-like Illness

Expanded PCR Panel Testing for Identification of Respiratory Pathogens and Coinfections in Influenza-like Illness

Antibiotic treatment of community-acquired and hospital-acquired pneumonia

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