Multiplex cytokine profile from dengue patients: MIP-1beta and IFN-gamma as predictive factors for severity

Abstract

Background: Dengue virus pathogenesis is not yet fully understood and the identification of patients at high risk for developing severe disease forms is still a great challenge in dengue patient care. During the present study, we evaluated prospectively the potential of cytokines present in plasma from patients with dengue in stratifying disease severity.

CLEC5A is critical for dengue virus–induced inflammasome activation in human macrophages

“…9 In addition to IL-1␤, the secretion of IL-18 is also dependent on activation of inflammasome and caspase-1. Because serum levels of IL-1␤ and IL-18 correlate with the severity of dengue infection, 10,11 these observations suggest that inflammasome activation may play critical roles in the pathogenesis of dengue infection. However, the mechanism by which DV activates inflammasomes to induce the secretion of IL-1␤ and IL-18 has not been well elucidated to date.…”

Dengue-2 and yellow fever 17DD viruses infect human dendritic cells, resulting in an induction of activation markers, cytokines and chemokines and secretion of different TNF-α and IFN-α profiles

“…These data are informative with respect to Th-1/Th-2 axis determination by surface markers on DCs after flavivirus infection. CD38 upregulation and OX40L downregulation indicate that both viruses favour a Th-1 type response during the early immune response, which likely plays a role in virus clearance, although a Th-2 response may be present as well during infections by both viruses (Bozza et al 2008, Querec et al 2009). …”

“…MoDCs -Cytokines and chemokines play important roles in dengue physiopathology (Bozza et al 2008, Noisakran & Perng 2008. Since DCs are one of the main sources for these molecules early in viral infection, we investigated the production of several of them after MoDC infection by DENV2 and YF17DD from 24-120 hpi.…”

“…For dengue fever, tumor necrosis factor (TNF)-α (Hober et al 1993, Braga et al 2001, interleukin (IL)-10 ( Green et al 1999, Azeredo et al 2001, IL-6 (Nguyen et al 2004), macrophage inhibitory factor , Assuncao-Miranda et al 2010 and interferon (IFN)-γ (Bozza et al 2008), among others (Srikiatkhachorn & Green 2010), have been reported. Chemokines also have an important role in pathogenesis.…”

“…Chemokines also have an important role in pathogenesis. For example, IP-10/CXCL-10 was found to be elevated in dengue haemorrhagic fever (DHF) (Fink et al 2007) and MCP-1/CCL-2 ( Lee et al 2006), while MIP-1β/CCL-4 is associated with a good prognosis (Bozza et al 2008). Elevated serum levels of IL-6, IL-8, TNF-α, MCP-1, IL-1 receptor agonist (Ra) and IL-10 were observed in fatal YF cases when compared with non-fatal cases (Bae et al 2008).…”