Multiplex detection of bacterial pathogens by PCR/SERS assay

Lyu, Nana; Potluri, Phani Rekha; Rajendran, Vinoth Kumar; Wang, Yuling; Sunna, Anwar

doi:10.1039/d4an00037d

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2024

DOI: 10.1039/d4an00037d

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Multiplex detection of bacterial pathogens by PCR/SERS assay

Nana Lyu,

Phani Rekha Potluri,

Vinoth Kumar Rajendran

et al.

Abstract: Bacterial infections are the leading cause of death globally. The detection of DNA sequences correlating to causative pathogen has become a vital tool in medical diagnostics. In practice, PCR-based assays...

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Cited by 2 publications

References 39 publications

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Zhang,

Hou,

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Localized surface plasmon resonance sensing based on monometallic gold nanoparticles: from material preparation to detection of bioanalytes

Zhang,

Hou,

et al. 2025

Anal. Methods

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Research progress in fluorescent biosensor technology for detecting Escherichia coli based on PCR

Guo,

Li,

Yang

et al. 2024

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Purpose In recent decades, advancements in biosensors technology have made fluorescent biosensor pivotal for biomolecular recognition. This paper aims to provide an in-depth analysis of polymerase chain reaction (PCR) fluorescent biosensor detection technology for identifying Escherichia coli (E. coli), setting the stage for future developments in the field. Design/methodology/approach The review of literature on PCR fluorescent biosensor detection technology for E. coli over the past decades includes discussions on traditional biological fluorescent detection, quantitative PCR fluorescent detection and digital fluorescent detection technology. Findings Advancements in fluorescent biosensor technology enable precise measurement of fluorescent signals, and when integrated with microfluidic technology, produce compact, reagent-efficient digital sensor devices. Originality/value This paper provides a comprehensive review of recent fluorescent detection technology for pathogenic E. coli, assessing method efficiencies and offering insights to advance the field.

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Multiplex detection of bacterial pathogens by PCR/SERS assay

Abstract: Bacterial infections are the leading cause of death globally. The detection of DNA sequences correlating to causative pathogen has become a vital tool in medical diagnostics. In practice, PCR-based assays...

Cited by 2 publications

References 39 publications

Localized surface plasmon resonance sensing based on monometallic gold nanoparticles: from material preparation to detection of bioanalytes

Localized surface plasmon resonance sensing based on monometallic gold nanoparticles: from material preparation to detection of bioanalytes

Research progress in fluorescent biosensor technology for detecting Escherichia coli based on PCR

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