Multiplex digital PCR: a superior technique to qPCR for the simultaneous detection of duck Tembusu virus, duck circovirus, and new duck reovirus

Yin, Yanwen; Xiong, Chenyong; Shi, Kaichuang; Long, Feng; Feng, Shuping; Qu, Shuping; Lu, Wei; Huang, M. Z.; Lin, Cheng‐Fang; Sun, Wenchao; Li, Zongqiang

doi:10.3389/fvets.2023.1222789

Cited by 5 publications

(1 citation statement)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The developed multiplex digital PCR (dPCR), compared to multiplex qPCR, can specifically detect DTMUV, DuCV, and NDRV but cannot amplify MDRV, MDPV, Goose Parvovirus (GPV), H4 Avian Influenza Virus (H4 AIV), H6 Avian Influenza Virus (H6 AIV), or NDV. Multiplex dPCR exhibits high sensitivity, with a detection limit as low as 1.3 copies/µL, which is 10 times lower than that of multiplex qPCR [101].…”

Section: Diagnostic Methodsmentioning

confidence: 94%

Advancements in Research on Duck Tembusu Virus Infections

Cheng,

Wang,

et al. 2024

Viruses

View full text Add to dashboard Cite

Duck Tembusu Virus (DTMUV) is a pathogen of the Flaviviridae family that causes infections in poultry, leading to significant economic losses in the duck farming industry in recent years. Ducks infected with this virus exhibit clinical symptoms such as decreased egg production and neurological disorders, along with serious consequences such as ovarian hemorrhage, organ enlargement, and necrosis. Variations in morbidity and mortality rates exist across different age groups of ducks. It is worth noting that DTMUV is not limited to ducks alone; it can also spread to other poultry such as chickens and geese, and antibodies related to DTMUV have even been found in duck farm workers, suggesting a potential risk of zoonotic transmission. This article provides a detailed overview of DTMUV research, delving into its genomic characteristics, vaccines, and the interplay with host immune responses. These in-depth research findings contribute to a more comprehensive understanding of the virus’s transmission mechanism and pathogenic process, offering crucial scientific support for epidemic prevention and control.

show abstract

Section: Diagnostic Methodsmentioning

confidence: 94%

Advancements in Research on Duck Tembusu Virus Infections

Cheng,

Wang,

et al. 2024

Viruses

View full text Add to dashboard Cite

show abstract

Chip-based digital PCR as a direct quantification method for residual DNA in mRNA drugs

Fan,

Zhao,

et al. 2024

Journal of Pharmaceutical and Biomedical Analysis

View full text Add to dashboard Cite

Development and application of quadruplex real time quantitative PCR method for differentiation of Muscovy duck parvovirus, Goose parvovirus, Duck circovirus, and Duck adenovirus 3

Wang,

Chen,

et al. 2024

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

IntroductionMuscovy duck parvovirus (MDPV), Goose parvovirus (GPV), Duck circovirus, (DuCV) and Duck adenovirus 3 (DAdV-3) are important pathogens that cause high morbidity and mortality in ducks, causing huge economic loss for the duck industry.MethodsThe present study, a quadruplex one-step real time quantitative PCR method for the detection of MDPV, GPV, DuCV, and DAdV-3 was developed. ResultsThe results showed that assay had no cross-reactivity with other poultry pathogens [Duck plague virus (DPV), Duck tembusu virus (DTMUV), H6 avian influenza virus (H6 AIV), New duck reovirus (NDRV), Newcastle disease virus (NDV), H4 avian influenza virus (H4 AIV), Escherichia coli (E. coli), Muscovy duck reovirus (MDRV), Egg drop syndrome virus (EDSV), Pasteurella multocida (P. multocida)]. The sensitivity result showed that the limits of detection for MDPV, GPV, DuCV, and DAdV-3 were 10, 10, 1 and 10 copies/µl, respectively; The coefficients of variation intra- and inter-method was 1-2%; The range of linear (109 to 103 copies/µL) demonstrated the R2 values for MDPV, GPV, DuCV, and DAdV-3 as 0.9975, 0.998, 0.9964, and 0.996, respectively. The quadruplex real time quantitative PCR method efficiency was 90.30%, 101.10%, 90.72%, and 90.57% for MDPV, GPV, DuCV, and DAdV-3, respectively. 396 clinical specimens collected in some duck sausages from June 2022 to July 2023 were simultaneously detected using the established quadruplex real time quantitative PCR method and the reported assays. The detection rates for MDPV, GPV, DuCV, and DAdV-3 were 8.33% (33/396), 17.93% (71/396), 33.58% (133/396), and 29.04% (115/396), respectively. The agreement between these assays was greater than 99.56%.DiscussionThe developed quadruplex real-time quantitative PCR assay can accurately detect these four viruses infecting ducks, providing a rapid, sensitive, specific and accurate technique for clinical testing.

show abstract

Multiplex digital PCR: a superior technique to qPCR for the simultaneous detection of duck Tembusu virus, duck circovirus, and new duck reovirus

Cited by 5 publications

References 43 publications

Advancements in Research on Duck Tembusu Virus Infections

Advancements in Research on Duck Tembusu Virus Infections

Chip-based digital PCR as a direct quantification method for residual DNA in mRNA drugs

Development and application of quadruplex real time quantitative PCR method for differentiation of Muscovy duck parvovirus, Goose parvovirus, Duck circovirus, and Duck adenovirus 3

Contact Info

Product

Resources

About