Multiplex electrochemiluminescence DNA sensor for determination of hepatitis B virus and hepatitis C virus based on multicolor quantum dots and Au nanoparticles

Liu, Linlin; Wang, Xinyan; Ma, Qiang; Lin, Zihan; Chen, Shufan; Li, Yang; Lu, Lehui; Qu, Hongping; Su, Xingguang

doi:10.1016/j.aca.2016.02.024

Cited by 65 publications

(21 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“… Detection Methods Detection Target Linear Range Detection Limit Ref. ECA HBV DNA 0.4–10 nM 0.01 nM 41 ECA HBsAg 1 ng –10 µg mL −1 0.14 ng mL −1 42 ECA HBsAg 0.05–15 ng mL −1 20 pg mL −1 22 ECA anti-HBc 1–6 ng mL −1 0.03 ng mL −1 21 ECLA HBV DNA 0.5 pM–0.5 nM 0.082 pM 43 ECA HBV preS1 1 pM–50 pM 0.1 pM This study Abbreviations: ECA, electrochemical assay; ECLA, electrochemiluminescence assay; HBsAg, hepatitis B surface antigen; anti-HBc, antibodies to hepatitis B core protein. …”

Section: Resultsmentioning

confidence: 99%

A Highly Sensitive Detection System based on Proximity-dependent Hybridization with Computer-aided Affinity Maturation of a scFv Antibody

Wang

Liang

et al. 2018

Sci Rep

View full text Add to dashboard Cite

The hepatitis B virus (HBV) infection is a critical health problem worldwide, and HBV preS1 is an important biomarker for monitoring HBV infection. Previously, we found that a murine monoclonal antibody, mAb-D8, targets the preS1 (aa91-107) fragment of HBV. To improve its performance, we prepared the single-chain variable region of mAb-D8 (scFvD8) and constructed the three-dimensional structure of the scFvD8-preS1 (aa91-107) complex by computer modelling. The affinity of scFvD8 was markedly increased by the introduction of mutations L96Tyr to Ser and H98Asp to Ser. Furthermore, a highly sensitive immunosensor was designed based on a proximity-dependent hybridization strategy in which the preS1 antigen competitively reacts with an antibody labelled with DNA, resulting in decreased proximity-dependent hybridization and increased electrochemical signal from the Fc fragment, which can be used for the quantisation of preS1. The results showed a wide detection range from 1 pM to 50 pM with a detection limit of 0.1 pM. The sensitivity and specificity of this immunosensor in clinical serum samples were 100% and 96%, respectively. This study provides a novel system based on proximity-dependent hybridization and the scFv antibody fragment for the rapid quantisation of antigens of interest with a high sensitivity.

show abstract

Section: Resultsmentioning

confidence: 99%

A Highly Sensitive Detection System based on Proximity-dependent Hybridization with Computer-aided Affinity Maturation of a scFv Antibody

Wang

Liang

et al. 2018

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Within the past five years, researchers have developed new B-CRSAs that take advantage of current technological advances, including functionalized carbon nanotube field effect transistor (FET) sensors, and metal oxide semiconductor (MOS) sensors, proposing devices with improved sensitivity, selectivity, and stability. With the development of more extensive and selective sensing mechanisms alongside advances in electronics and signal processing, B-CRSA devices have become smaller, more selective, and more sensitive, with fast data processing time and facile readout of vapor analysis [ 54 , 66 , 131 , 132 , 133 , 134 ].…”

Section: Future Directions and Remaining Challenges For B-crsa Diamentioning

confidence: 99%

Cutting Edge Methods for Non-Invasive Disease Diagnosis Using E-Tongue and E-Nose Devices

Fitzgerald

Fenniri

2017

Biosensors

View full text Add to dashboard Cite

Biomimetic cross-reactive sensor arrays (B-CRSAs) have been used to detect and diagnose a wide variety of diseases including metabolic disorders, mental health diseases, and cancer by analyzing both vapor and liquid patient samples. Technological advancements over the past decade have made these systems selective, sensitive, and affordable. To date, devices for non-invasive and accurate disease diagnosis have seen rapid improvement, suggesting a feasible alternative to current standards for medical diagnostics. This review provides an overview of the most recent B-CRSAs for diagnostics (also referred to electronic noses and tongues in the literature) and an outlook for future technological development.

show abstract

“…These were incubated with target DNA, which prevented the binding of target DNA coated AuNPs in a dose-dependent manner. Subsequently, target DNA concentrations were determined by the quenching of QD electrochemoluminescence by AuNPs [ 76 ]. By employing these methods, the authors could detect HBV DNA with concentrations as low as 8.2 × 10 −14 M in human serum without PCR amplification.…”

Section: Combinations Of Different Nanoparticlesmentioning

confidence: 99%

Applicability of Metal Nanoparticles in the Detection and Monitoring of Hepatitis B Virus Infection

Shevtsov

Zhao

Protzer

et al. 2017

Viruses

View full text Add to dashboard Cite

Chronic infection with the hepatitis B virus (HBV) can lead to liver failure and can cause liver cirrhosis and hepatocellular carcinoma (HCC). Reliable means for detecting and monitoring HBV infection are essential to identify patients in need of therapy and to prevent HBV transmission. Nanomaterials with defined electrical, optical, and mechanical properties have been developed to detect and quantify viral antigens. In this review, we discuss the challenges in applying nanoparticles to HBV antigen detection and in realizing the bio-analytical potential of such nanoparticles. We discuss recent developments in generating detection platforms based on gold and iron oxide nanoparticles. Such platforms increase biological material detection efficiency by the targeted capture and concentration of HBV antigens, but the unique properties of nanoparticles can also be exploited for direct, sensitive, and specific antigen detection. We discuss several studies that show that nanomaterial-based platforms enable ultrasensitive HBV antigen detection.

show abstract

Multiplex electrochemiluminescence DNA sensor for determination of hepatitis B virus and hepatitis C virus based on multicolor quantum dots and Au nanoparticles

Cited by 65 publications

References 49 publications

A Highly Sensitive Detection System based on Proximity-dependent Hybridization with Computer-aided Affinity Maturation of a scFv Antibody

A Highly Sensitive Detection System based on Proximity-dependent Hybridization with Computer-aided Affinity Maturation of a scFv Antibody

Cutting Edge Methods for Non-Invasive Disease Diagnosis Using E-Tongue and E-Nose Devices

Applicability of Metal Nanoparticles in the Detection and Monitoring of Hepatitis B Virus Infection

Contact Info

Product

Resources

About