Multiplex microsatellite PCR panels and their application in genetic analyses of bighead carp (Hypophthalmichthys nobilis) and silver carp (H. molitrix)

Abstract

In this study, three multiplex PCR panels involving twelve microsatellite loci were developed for cross‐species amplification in bighead carp (Hypophthalmichthys nobilis) and silver carp (H. molitrix). The panels were verified using population genetic analyses, and species and simulated hybrid discriminations based on the genotype data of all individuals from two populations for each species. The number of alleles (Na), the observed and expected heterozygosity (Ho and He), and the polymorphic information content (PIC) of twelve loci were ranged from 5 to 20, 0.189 to 0.956, 0.177 to 901, and 0.169 to 0.887, respectively. Overall, almost all the microsatellite loci were detected as highly polymorphic across the two species. The populations of same species showed close genetic distances and similar genetic structures. Significant genetic differentiations were only detected between populations from different species, with a higher variation between species (21.17%) compared with that between populations within species (0.18%). In the species and simulated hybrid discrimination analyses, the individuals from different species were separated distinctly, and the simulated hybrids could almost be separated with the true individuals. In brief, the results indicated the high efficiency of the microsatellite multiplex panels developed in this study, which provide a practicable tool for further genetic analyses of H. nobilis and H. molitrix.

Genetic Assessment of Silver Carp Populations in River Chenab (Pakistan) as Revealed by SSR Markers

“…Fang et al [41] developed 12 SSRs for cross-species amplification in silver carp (H. molitrix) and bighead carp (Hypophthalmichthys nobilis). The values of number of alleles (Na), the observed heterozygosity (Ho) and expected heterozygosity (He), and the polymorphic information content (PIC) vary from 5 to 20, 0.189-0.956, 0.177-901, and 0.169-0.887, respectively.…”