Multiplex PCR Assay for Identifi cation and Differentiation of Campylobacter jejuni and Campylobacter coli Isolates

Pavlova, Maria; Dobreva, Elina; Ivanova, K.; Asseva, G.; Ivanov, Ivan N.; Petrov, Peter K.; Velev, Valeri; Tomova, Ivelina I.; Tiholova, Maida M.; Kantardjiev, Todor

doi:10.1515/folmed-2016-0016

Cited by 11 publications

(7 citation statements)

References 17 publications

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“…Bacterial pathogens have been confirmed as the leading cause of acute diarrhea amongst outpatients in China ( Wang et al, 2015 ; Shen et al, 2016 ; Chang et al, 2017 ). Campylobacter infection is considered to be one of the leading causes of bacterial gastroenteritis in the developed countries ( Bresee et al, 2012 ; Wei et al, 2014 ; Kaakoush et al, 2015 ; Kumagai et al, 2015 ; Nohra et al, 2016 ; Pavlova et al, 2016 ). One comparable study reported no Campylobacter infections among diarrheal patients in a developed region neither in children (under 5 years, 0/1422)) nor adults (0/1047) ( Wang et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Prevalence and Molecular Characterization of Campylobacter spp. Isolated from Patients with Diarrhea in Shunyi, Beijing

Liu

Zhang

et al. 2018

Front. Microbiol.

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Bacterial pathogens have been confirmed as the major cause of acute diarrhea among outpatients in China. In this study, 370 stool samples from the patients aged from 15 to 87 years old with diarrhea were collected over 12 months (from May 2016 to April 2017) in two hospitals in Shunyi, Beijing. Bacterial isolation was performed for the common enteric pathogens: Campylobacter, Salmonella, Shigella, Diarrheagenic Escherichia coli, and Vibrio parahaemolyticus for 370 samples. The filtration method was used for the Campylobacter isolation in this study. The prevalence and molecular characterization of the Campylobacter were investigated. The isolation ratio for Campylobacter, Salmonella, Shigella, Diarrheagenic E. coli, and V. parahaemolyticus was 7.0% (26/370), 6.2% (23/370), 0.3% (1/370), 7.3% (27/370), and 10.3% (38/370), respectively. Based on the isolation result, Campylobacter positive cases presented in almost every month of the whole year and the isolation ratio was the highest among the tested pathogens during October to March. There was no significant difference between genders of Campylobacter positive cases. More Campylobacter positive cases presented dehydration compared with those who were positive for Salmonella. Twenty-six Campylobacter isolates were obtained in this study and 24 of these were Campylobacter jejuni. The antibiotic susceptibility tests indicated that 83.3% (20/24) of the isolates exhibited resistance to three or more types of antibiotic. Twenty STs were identified for the 26 Campylobacter isolates and four novel STs were identified in this study. No clonal cluster was found among these isolates. This is the first study for Campylobacter isolated using the filtration method in China which indicated the Campylobacter infection might be seriously under-ascertained in the diarrheal patients in China.

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Section: Discussionmentioning

confidence: 99%

Prevalence and Molecular Characterization of Campylobacter spp. Isolated from Patients with Diarrhea in Shunyi, Beijing

Liu

Zhang

et al. 2018

Front. Microbiol.

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“…Bacterial diarrhea is a serious public health problem worldwide, especially Campylobacter spp. considered as a leading cause (Lynch et al, 2011; Wei et al, 2014; Nohra et al, 2016; Pavlova et al, 2016). In this study, the isolation of Campylobacter is also being the most common pathogen among the tested five enteric bacterial pathogens with the ratio of 12.1% (23/190).…”

Section: Discussionmentioning

confidence: 99%

Comparison of the Filtration Culture and Multiple Real-Time PCR Examination for Campylobacter spp. From Stool Specimens in Diarrheal Patients

et al. 2018

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Campylobacter is one of the most common pathogens leading to the bacterial diarrheal illness. In order to set up one effective culture independent assay for the screen of the Campylobacter infection in the diarrheal patients, the quadruple real-time PCR method comparing to the culture based on the enriched filtration method which was recognized as the most effective isolation method was assessed for 190 stool samples from the diarrheal patients collected during the Foodborne Diseases Active Surveillance Network in Beijing. This multiple real-time PCR was designed to identify the Campylobacter genus, C. jejuni, C. coli, and C. lari simultaneously. With the enrichment culture method, 23 (12.1%, 23/190) Campylobacter isolates were obtained (20 C. jejuni and 3 C. coli), however, 31 samples (16.3%, 31/190) were detected positively with the real-time PCR (21 C. jejuni, 8 C. coli, and 2 Campylobacter genus only). With the comparison, the real-time-PCR method is more sensitive than the enrichment filtration method (16.3 vs. 12.1%, p = 0.021). Among the culture-positive samples, 95.7% (22/23) were detected positively by PCR which indicate the specificity of this method was higher. These two methods were consistent well (Kappa = 0.785, p < 0.05). Comparing to the culture methods, the result of the multiple real-time PCR method is sensitive, reliable and rapid. The present study indicated this multiple real-time PCR can be used both for the surveillance network and the preceding screen for bacteria isolation. This is first comparative study between the culture and multiple real-time PCR method for Campylobacter identification in stool specimens from the diarrheal patients.

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“…Although the reported incidence of campylobacteriosis remained low in Bulgaria, a research study performed between February 2014 and January 2015 examining patients with diarrheal syndrome reported high detection rate of Campylobacter from their stool samples, in which 43% (40/93) of the samples examined were PCR positive for Campylobacter ( Pavlova et al., 2016 ). Furthermore, a recent study examining the presence of Campylobacter in children with acute diarrhea under five years of age reported a high infection rate, raising concerns regarding Campylobacter infection in paediatric patients.…”

Section: Epidemiologymentioning

confidence: 99%

Global epidemiology of campylobacteriosis and the impact of COVID-19

Liu

Lee

Xue

et al. 2022

Front. Cell. Infect. Microbiol.

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Campylobacteriosis is a gastroenteritis caused by pathogenic Campylobacter species and an important topic in public health. Here we review the global epidemiology of campylobacteriosis in the last eight years between 2014-2021, providing comprehensive and updated information on the reported incidence and outbreaks of Campylobacter infections. The government public health website of each of the 195 countries and publications from 2014 to September 2022 in public databases were searched. The reported incidence of campylobacteriosis in pre-COVID-19 years was compared to that during the COVID-19 pandemic in countries where data were available. Czech Republic had the highest reported incidence of campylobacteriosis worldwide (215 per 100,000 in 2019), followed by Australia (146.8 per 100,000 in 2016) and New Zealand (126.1 per 100,000 in 2019). Campylobacter was one of the most common human enteric pathogens in both developed and developing countries. About 90% of cases of campylobacteriosis were caused by Campylobacter jejuni, whereas less than 10% of cases were caused by Campylobacter coli. Other Campylobacter species were also isolated. The reported incidence and case numbers of campylobacteriosis in developed nations have remained steadily high prior to the COVID-19 pandemic, whilst some countries reported an increasing trend such as France and Japan. While outbreaks were more frequently reported in some countries, Campylobacter infections were mainly sporadic cases in most of the developed countries. Campylobacter infection was more common in summer in some but not all countries. Campylobacter infection was more common in males than females. The COVID-19 pandemic has reduced the reported incidence of campylobacteriosis in most countries where 2020 epidemiology data were available. In conclusion, Campylobacter infection remains a global health concern. Increased research and improved strategies are needed for prevention and reduction of Campylobacter infection.

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Multiplex PCR Assay for Identifi cation and Differentiation of Campylobacter jejuni and Campylobacter coli Isolates

Abstract: The multiplex PCR assay is a fast, accurate method for identifi cation of Campylobacter spp. which makes it quite necessary in the clinical diagnostic practice.

Cited by 11 publications

References 17 publications

Prevalence and Molecular Characterization of Campylobacter spp. Isolated from Patients with Diarrhea in Shunyi, Beijing

Prevalence and Molecular Characterization of Campylobacter spp. Isolated from Patients with Diarrhea in Shunyi, Beijing

Comparison of the Filtration Culture and Multiple Real-Time PCR Examination for Campylobacter spp. From Stool Specimens in Diarrheal Patients

Global epidemiology of campylobacteriosis and the impact of COVID-19

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