Multiplex PCR assay for malaria vector <i>Anopheles minimus</i> and four related species in the Myzomyia Series from Southeast Asia

Phuc, Hoang Kim; Ball, Amanda; Sơn, Lê Hoàng; Hanh, N. V.; Tu, N. D.; Lien, Ngo Giang; Verardi, Andrea Antonio; Townson, Harold

doi:10.1111/j.1365-2915.2003.00462.x

Cited by 68 publications

(55 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Adults are similar to those of the Minimus Subgroup and the two taxa exhibit overlapping characters. Consequently, molecular techniques have been developed for differentiating these species [149-152]. …”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

The dominant Anopheles vectors of human malaria in the Asia-Pacific region: occurrence data, distribution maps and bionomic précis

Bangs²,

et al. 2011

View full text Add to dashboard Cite

BackgroundThe final article in a series of three publications examining the global distribution of 41 dominant vector species (DVS) of malaria is presented here. The first publication examined the DVS from the Americas, with the second covering those species present in Africa, Europe and the Middle East. Here we discuss the 19 DVS of the Asian-Pacific region. This region experiences a high diversity of vector species, many occurring sympatrically, which, combined with the occurrence of a high number of species complexes and suspected species complexes, and behavioural plasticity of many of these major vectors, adds a level of entomological complexity not comparable elsewhere globally. To try and untangle the intricacy of the vectors of this region and to increase the effectiveness of vector control interventions, an understanding of the contemporary distribution of each species, combined with a synthesis of the current knowledge of their behaviour and ecology is needed.ResultsExpert opinion (EO) range maps, created with the most up-to-date expert knowledge of each DVS distribution, were combined with a contemporary database of occurrence data and a suite of open access, environmental and climatic variables. Using the Boosted Regression Tree (BRT) modelling method, distribution maps of each DVS were produced. The occurrence data were abstracted from the formal, published literature, plus other relevant sources, resulting in the collation of DVS occurrence at 10116 locations across 31 countries, of which 8853 were successfully geo-referenced and 7430 were resolved to spatial areas that could be included in the BRT model. A detailed summary of the information on the bionomics of each species and species complex is also presented.ConclusionsThis article concludes a project aimed to establish the contemporary global distribution of the DVS of malaria. The three articles produced are intended as a detailed reference for scientists continuing research into the aspects of taxonomy, biology and ecology relevant to species-specific vector control. This research is particularly relevant to help unravel the complicated taxonomic status, ecology and epidemiology of the vectors of the Asia-Pacific region. All the occurrence data, predictive maps and EO-shape files generated during the production of these publications will be made available in the public domain. We hope that this will encourage data sharing to improve future iterations of the distribution maps.

show abstract

Section: Resultsmentioning

confidence: 99%

“…pampanai Büttiker & Beales and An. varuna Iyengar [151,152]. Moreover, the many older records of An.…”

Section: Resultsmentioning

confidence: 99%

The dominant Anopheles vectors of human malaria in the Asia-Pacific region: occurrence data, distribution maps and bionomic précis

Bangs²,

et al. 2011

View full text Add to dashboard Cite

show abstract

“…100 for molecular analysis, 100 for rearing, and 100 for dissection Lopez et al (2003) list multiplex PCR methods among the innovative tools currently used in pathogen diagnosis based on the sensitivity of the technique and the ability to simultaneously detect different DNA targets in a single reaction. In insect parasitology, molecular diagnostic tools incorporating multiplex PCR have been used to identify different mosquito species and strains that vector malaria (Kengne et al 2001;Phuc et al 2003), to detect animal viruses and plant pathogens in insect vectors (Wilson & Chase 1993;Rodrigues et al 2003), to identify microsporidian infection in ants (Valles et al 2002) and bacterial infection in insect cadavers (Bourque et al 1993). However, the implementation of multiplex assays to detect and identify insect parasitoid species within their hosts has not previously been reported.…”

Section: Discussionmentioning

confidence: 99%

A single-step multiplex PCR assay for the detection of EuropeanPeristenusspp., parasitoids ofLygusspp.

Gariépy

Kuhlmann

Haye

et al. 2005

Biocontrol Science and Technology

View full text Add to dashboard Cite

Lygus Hahn (Hemiptera: Miridae) are serious pests of agricultural and greenhouse crops throughout North America. In Europe, bivoltine Peristenus Fö rster (Hymenoptera: Braconidae) species have a significant impact on Lygus populations. Release and establishment of European P. digoneutis Loan in Lygus lineolaris (Palisot de Beauvois) populations in northeastern USA has renewed interest in the intended liberation of European parasitoids for Lygus control in Canada. Accurate identification of natural enemies is the cornerstone of biological control but conventional methods for identifying Peristenus species and estimating parasitism rates rely on tedious and time-consuming dissection and rearing methods. The present study describes species-specific PCR primers for three species of Peristenus , and the use of a multiplex PCR assay to detect P. digoneutis and P. stygicus Loan eggs and larvae from Lygus rugulipennis Poppius nymphs. Results indicate that the primers amplify uniquely sized, species-specific PCR products for the three species and are capable of detecting single eggs in parasitized nymphs within 3 days post-parasitism. Using a multiplex PCR assay, the primers maintain specificity and sensitivity, and allow detection of each of the three species in a single reaction. Although molecular diagnostics have previously been used in the identification of parasitoids and estimation of parasitism rates, this is the first time a single-step multiplex PCR protocol has been described.

show abstract

“…Past applications of DNA-based species identification range from reconstructing food webs by identifying fragments in stomachs (Symondson 2002) to recognizing products prepared from protected species (Palumbi and Cipriano 1998) and resolving complexes of mosquitoes that transmit malaria and dengue fever (Phuc et al 2003). Despite such demonstrations, the lack of a lingua franca has limited the use of DNA as a general tool for species identifications.…”

Section: Introductionmentioning

confidence: 99%

Identification of Birds through DNA Barcodes

et al. 2004

View full text Add to dashboard Cite

Short DNA sequences from a standardized region of the genome provide a DNA barcode for identifying species. Compiling a public library of DNA barcodes linked to named specimens could provide a new master key for identifying species, one whose power will rise with increased taxon coverage and with faster, cheaper sequencing. Recent work suggests that sequence diversity in a 648-bp region of the mitochondrial gene, cytochrome c oxidase I (COI), might serve as a DNA barcode for the identification of animal species. This study tested the effectiveness of a COI barcode in discriminating bird species, one of the largest and best-studied vertebrate groups. We determined COI barcodes for 260 species of North American birds and found that distinguishing species was generally straightforward. All species had a different COI barcode(s), and the differences between closely related species were, on average, 18 times higher than the differences within species. Our results identified four probable new species of North American birds, suggesting that a global survey will lead to the recognition of many additional bird species. The finding of large COI sequence differences between, as compared to small differences within, species confirms the effectiveness of COI barcodes for the identification of bird species. This result plus those from other groups of animals imply that a standard screening threshold of sequence difference (10× average intraspecific difference) could speed the discovery of new animal species. The growing evidence for the effectiveness of DNA barcodes as a basis for species identification supports an international exercise that has recently begun to assemble a comprehensive library of COI sequences linked to named specimens.

show abstract

Multiplex PCR assay for malaria vector Anopheles minimus and four related species in the Myzomyia Series from Southeast Asia

Cited by 68 publications

References 16 publications

The dominant Anopheles vectors of human malaria in the Asia-Pacific region: occurrence data, distribution maps and bionomic précis

The dominant Anopheles vectors of human malaria in the Asia-Pacific region: occurrence data, distribution maps and bionomic précis

A single-step multiplex PCR assay for the detection of EuropeanPeristenusspp., parasitoids ofLygusspp.

Identification of Birds through DNA Barcodes

Contact Info

Product

Resources

About