Multiplex PCR for cryptococcal meningoencephalitis – calling for caution!!!

Abstract

An early diagnosis of cryptococcal meningoencephalitis is needed for prevention of significant morbidity and mortality. We present two cases of culture positive cryptococcal meningoencephalitis where the cerebrospinal fluid (CSF) molecular PCR panel failed to detect the Cryptoccoccus resulting in delay in diagnosis. Careful interpretation of such molecular platforms and multiple testing methods including CSF cryptococcal antigen (CrAG) and cultures are advised in suspected cryptococcal meningitis.