Multiplex PCR for rapid diagnosis of tuberculous meningitis

Sharma, Kusum; Sharma, Aman; Ray, Pallab; Kumar, Sharma Shiv; Modi, Manish; Sudesh, Prabhakar; Varma, Subhash; Sharma, Mukut

doi:10.1007/s00415-011-6010-4

Cited by 67 publications

(71 citation statements)

References 22 publications

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“…None of the four samples with NTM isolation was positive for mpt64, showing the specificity of this target to MTBC detection, as reported by Takahashi & Nakayama (2006), Kusum et al (2012), Sethi et al (2012) and Nimesh et al (2013). All of the patients infected with NTM were notified and treated for TB.…”

Section: Discussionsupporting

confidence: 70%

“…Many regions of the mycobacterial genome have been used in molecular diagnosis of TB, such as IS6110, 16S rRNA, hsp65, rpoB, sdaA, devR and mpt64 (Manjunath et al, 1991;Lachnik et al, 2002;Kim et al, 2004;Chakravorty et al, 2005;Negi et al, 2007;Hwang et al, 2013;Nimesh et al, 2013). mpt64 is found as a single copy in the genome of MTBC species (Lee et al, 1994), and has been used for both pulmonary and extrapulmonary TB diagnosis (Tan et al, 1995;Dar et al, 1998;Martins et al, 2000;Bhanu et al, 2005;Therese et al, 2005;Takahashi & Nakayama, 2006;Dil-Afroze et al, 2008;Kusum et al, 2012;Sethi et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Rapid detection of Mycobacterium tuberculosis complex by real-time PCR in sputum samples and its use in the routine diagnosis in a reference laboratory

Pinhata

Cergole-Novella²,

Carmo³

et al. 2015

Journal of Medical Microbiology

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Tuberculosis (TB) is an infectious disease of global distribution, constituting a serious public health problem in Brazil. Sã o Paulo State, located in the south-east of Brazil, notified 16 580 new TB cases in 2013. The Instituto Adolfo Lutz is a public health reference laboratory for TB diagnosis for all the State. Considering that rapid and accurate diagnosis is essential for TB control, the aim of this study was to evaluate the use of an in-house real-time (RT)-PCR assay targeting the mpt64 gene in the routine diagnosis of TB, and to compare this technique with smear microscopy and culture. From August 2012 to October 2013, 715 sputum samples from 657 patients were included in the study. Smear microscopy, culture, phenotypic and PRAhsp65 identification of mycobacteria, and mpt64 RT-PCR were performed. With respect to confirmed TB cases (n562/657; 9.4 %), smear microscopy had a sensitivity of 82.3 %. Culture and RT-PCR showed the same sensitivity, i.e. 90.3 %. Specificity was 99.7, 99.4 and 98.6 % for smear microscopy, culture and RT-PCR, respectively. mpt64 RT-PCR showed high sensitivity and specificity for the detection of Mycobacterium tuberculosis complex in sputum samples. This technique can be deployed in laboratories that do not have a rapid test for TB available, enabling the performance of TB diagnosis in up to 5 h.

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Section: Discussionsupporting

confidence: 70%

Section: Introductionmentioning

confidence: 99%

Rapid detection of Mycobacterium tuberculosis complex by real-time PCR in sputum samples and its use in the routine diagnosis in a reference laboratory

Pinhata

Cergole-Novella²,

Carmo³

et al. 2015

Journal of Medical Microbiology

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“…The low sensitivity of this PCR test was attributed to the utilisation of a single target for amplification, resulting in falsenegative results (5). Newer PCR tests, called Multiplex PCRs, allow for the amplification of several target genes (IS6110, protein b, and MPB64) simultaneously, and this has helped to increase sensitivity to a level of 85-95% and specificity to 100% (6). The patient's CSF results were positive when employing a Multiplex PCR for tuberculosis.…”

Section: Case Presentationmentioning

confidence: 87%

Rare Clinical Presentation of Tuberculous Meningitis: A Case Report

Tan

Nordin

Besari

2017

MJMS

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“…CSF adenosine deaminase can be used as a reasonable diagnostic tool [6] but demonstration of the organism by culture remains the gold standard. PCR for TB can be used as substitute to culture with equivalent sensitivity and specificity as it gives rapid results on which treatment decision can be banked [7].…”

Section: Discussionmentioning

confidence: 99%

Temporal Lobe Encephalitis Need not Always be Herpes Simplex Encephalitis: Think of Tuberculosis

Madireddi

Reddy

Stanley

et al. 2016

JCDR

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A 36-year-old male, car driver, with no significant past medical history was brought in altered sensorium of one day duration. He had a prodrome of headache and fever preceding this for 3 days. He also had an episode of seizures of generalized tonic-clonic type. There was no history of trauma. His GCS was 14, E4V4M6. He was in altered sensorium and agitated. Cranial, motor and sensory nervous system examination was normal. Kernig's and Brudzinski were positive. Diagnosis of Meningo-encephalitis was considered. Blood investigations were normal. Imaging showed T2W and FLAIR hyperintensities, restricted diffusion and patchy enhancement post contrast in bilateral medial temporal lobe (L>R) [Table/ Fig-1], the left insular cortex [Table/ Fig-2], bilateral inferior frontal lobes (L>R) and bilateral cingulate gyri. Electroencephalogram revealed sharp wave discharge in T4 to T6 leads. Cerebrospinal fluid analysis was predominantly lymphocytic with high protein and low-normal glucose. Diagnosis of Herpes simplex virus (HSV) encephalitis was strongly considered. He was started on I.V acyclovir impending CSF analysis culture and Polymerase Chain Reaction (PCR) reports. Patient continued to have fever, He later developed global aphasia and his GCS worsened to 9, E4V1M4. His power decreased to 3/5 in the lower limbs. In the milieu of his worsening clinical condition,

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Multiplex PCR for rapid diagnosis of tuberculous meningitis

Cited by 67 publications

References 22 publications

Rapid detection of Mycobacterium tuberculosis complex by real-time PCR in sputum samples and its use in the routine diagnosis in a reference laboratory

Rapid detection of Mycobacterium tuberculosis complex by real-time PCR in sputum samples and its use in the routine diagnosis in a reference laboratory

Rare Clinical Presentation of Tuberculous Meningitis: A Case Report

Temporal Lobe Encephalitis Need not Always be Herpes Simplex Encephalitis: Think of Tuberculosis

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