Multiplex real-time PCR for identification of canine parvovirus antigenic types

Kaur, Gurpreet; Chandra, Mudit; Dwivedi, P. N.; Narang, Deepti

doi:10.1016/j.jviromet.2016.02.013

Cited by 18 publications

(9 citation statements)

References 20 publications

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“…Several assays have been reported for the detection or quantitation of CPV DNA [12][13][14][15][16][17][18], including PCR, nested PCR, iiPCR, RPA, LAMP-ELISA, LAMP-LFD, LAMP, polymerase spiral reaction, and SYBR Green based real-time PCR, but none of these assays enable differentiation CPV antigenic types and CPV-like viruses (MEV and FPV). Several other assays have been reported for the detection and differentiation of type 2-based vaccines and field strains of CPV [19] or typing of three antigenic types of CPV [20] or CPV and MEV [21] assay and MGB probe real-time RT-PCR, but none of these assays enables simultaneous detection and differentiation of four antigenic types of CPV.…”

Section: Introductionmentioning

confidence: 99%

A multiplex TaqMan real-time PCR for detection and differentiation of four antigenic types of canine parvovirus in China

Sun

Cheng

Lin

et al. 2018

Molecular and Cellular Probes

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Canine parvovirus (CPV) is an important pathogen in domestic dogs, and the original antigenic types CPV-2 and its variants, CPV-2a, 2b and 2c, are prevalent worldwide. A multiplex TaqMan real-time PCR method was developed for the detection and differentiation of four antigenic types of CPV. A set of primers and probes, CPV-305F/CPV-305R and CPV-2-305P (for CPV-2)/CPV-2a-305P (for CPV-2a, 2b and 2c), was able to differentiate CPV-2 and its variants (CPV-2a, 2b and 2c). Another set of primers and probes, CPV-426F/CPV-426R and CPV-2-426P (for CPV-2 and 2a)/CPV-2b-426P (for CPV-2b)/CPV-2c-426P (for CPV-2c), was able to differentiate CPV-2a (2), CPV-2b, and CPV-2c. With these primers and probes, the multiplex TaqMan real-time PCR assay detected effectively and differentiated CPV-2, 2a, 2b and 2c by two separate real-time PCRs. No cross reactivity was observed with canine distemper virus, canine adenovirus, and canine coronavirus. The detection limit of the assay is 10 genome copies/μL for CPV-2, CPV-2a, CPV-2b, and 10 copies/μL for CPV-2c. The multiplex real-time PCR has 100% agreement with DNA sequencing. We provide a sensitive assay that simultaneously detects and differentiate four antigenic types of CPV and the method was also used for quantification of CPVs viral genome.

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Section: Introductionmentioning

confidence: 99%

A multiplex TaqMan real-time PCR for detection and differentiation of four antigenic types of canine parvovirus in China

Sun

Cheng

Lin

et al. 2018

Molecular and Cellular Probes

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“…DNA sequencing is the gold standard for identifying the CPV‐2 genotype, despite the fact that it is time‐consuming, costly and subject to artificial contamination. Other methods can be used to genotype CPV‐2, such as the MGB probe assay, multiplex real‐time PCR assay and the high‐resolution melting (HRM) curve method . However, multiplex real‐time PCR and HRM curve method require two to three primer pairs and take at least 4 hours .…”

Section: Discussionmentioning

confidence: 99%

“…Other methods can be used to genotype CPV‐2, such as the MGB probe assay, multiplex real‐time PCR assay and the high‐resolution melting (HRM) curve method . However, multiplex real‐time PCR and HRM curve method require two to three primer pairs and take at least 4 hours . In addition, due to the very short range of melting temperature (0.2‐0.6°C), using HRM analysis to differentiate between different genotypes of CPV‐2 has its limitation .…”

Section: Discussionmentioning

confidence: 99%

A SimpleProbe^® real‐time PCR assay for differentiating the canine parvovirus type 2 genotype

Hoang

Lien

et al. 2018

Clinical Laboratory Analysis

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“…In India and Iran, the dominant types in recent outbreaks were CPV-2a and -2b [21,72,73] . In 2016, CPV-2c was detected in northern and central India [74,75] and Iran [73] .…”

Section: Prevalence Of Canine Parvovirus Type 2 Variantsmentioning

confidence: 99%

Canine Parvoviruslar Üzerine Güncelleme: Canine Konakçıyı Etkileyen Genetik Varyasyonlara Vurgu Yaparak Moleküler ve Genomik Yönler

Vannamahaxay¹,

Chuammitri²

2017

Kafkas Univ Vet Fak Derg

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Citation of This ArticleVannamahaxay S, Chuammitri P: Update on canine Parvovirus: Molecular and genomic aspects, with emphasis on genetic variants affecting the canine host. Kafkas Univ Vet Fak Derg, 23 (5): 847-856, 2017847-856, . DOI: 10.9775/kvfd.2017 AbstractCanine parvovirus (CPV), the etiology of hemorrhagic enteritis in dogs, was first isolated as CPV type 2 (CPV-2) almost 40 years ago, and was soon replaced by the emergence of new variant types. The major viral capsid proteins encoded by the VP2 gene are the sites where amino acids are often substituted, accounting for the unusual nature of this type of DNA virus. The alteration of specific residues has contributed to different antigenic variants which have affected the evolution of virus binding and host immunity to this virus. Sequence analysis of the VP2 gene and subsequent characterization have revealed three circulating CPV-2 strains, CPV-2a, CPV-2b, and CPV-2c, identified by mutations at amino acid residue 426. The latter strain displays increased pathogenicity in dogs and an extended host range. The present review article aimed at updating contemporary information on epidemiological studies and surveys from CPV field work. Moreover, we pointed out some sensitive and rapid diagnostic tools for detecting CPV in clinical samples, techniques which will be useful for health monitoring and management of CPV with currently available vaccines. Keywords: Canine Parvovirus, CPV type 2, Genetic Variation, VP2 Gene, Mutation, Dog Köpek Parvovirusu Üzerine Bir Güncelleme: Köpek Konakçıya Etki Eden Genetik Varyasyonların Moleküler ve Genomik Özellikleri ÖzetKöpeklerde hemorajik enteritin etiyolojik etkeni olan Canine parvovirus (CPV) neredeyse 40 yıl önce ilk olarak CPV tip 2 (CPV-2) olarak izole edildi ve hemen sonrasında ortaya çıkan yeni varyant tipler CPV tip 2'nin yerine geçti. VP2 geni tarafından kodlanan major viral kapsid proteinler aynı zamanda en sıklıkla amino asitlerin başka amino asitlerle değiştiği alanlar olup bu tip DNA virusların aykırı doğasının da sebebini oluşturmaktadır. Spesifik yapılardaki değişimler farklı antijenik varyantların oluşmasına katkıda bulunarak bu virüsün bağlanma ve virusa karşı konakçı bağışıklığının değişmesini etkilemiştir. VP2 geninin sekans analizi ve takibinde karakterizasyonu, 426. amino asitte mutasyon ile şekillenen CPV2a, CPV-2b ve CPV-2c olmak üzere dolaşımda 3 farklı CPV-2 suşunun olduğunu göstermiştir. CPV-2c köpeklerde artmış patojenite ve daha geniş konakçı yelpazesi göstermektedir. Bu derlemede güncel epidemiyolojik çalışmalar ile CPV saha çalışmaları hakkındaki bilgilerin güncellenmesi amaçlanmıştır. Ayrıca, klinik örneklerde CPV'nin tanısında kullanılmak suretiyle sağlık taramasında faydalı olabilecek ve mevcut aşılarla CPV'nin kontrol altına alınmasında faydalı olabilecek bazı hassas ve hızlı tanı yöntemleri değerlendirilmiştir.

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Multiplex real-time PCR for identification of canine parvovirus antigenic types

Cited by 18 publications

References 20 publications

A multiplex TaqMan real-time PCR for detection and differentiation of four antigenic types of canine parvovirus in China

A multiplex TaqMan real-time PCR for detection and differentiation of four antigenic types of canine parvovirus in China

A SimpleProbe^® real‐time PCR assay for differentiating the canine parvovirus type 2 genotype

Canine Parvoviruslar Üzerine Güncelleme: Canine Konakçıyı Etkileyen Genetik Varyasyonlara Vurgu Yaparak Moleküler ve Genomik Yönler

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Multiplex real-time PCR for identification of canine parvovirus antigenic types

Cited by 18 publications

References 20 publications

A multiplex TaqMan real-time PCR for detection and differentiation of four antigenic types of canine parvovirus in China

A multiplex TaqMan real-time PCR for detection and differentiation of four antigenic types of canine parvovirus in China

A SimpleProbe® real‐time PCR assay for differentiating the canine parvovirus type 2 genotype

Canine Parvoviruslar Üzerine Güncelleme: Canine Konakçıyı Etkileyen Genetik Varyasyonlara Vurgu Yaparak Moleküler ve Genomik Yönler

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A SimpleProbe^® real‐time PCR assay for differentiating the canine parvovirus type 2 genotype