Multiplex Real-time PCR for RRM1, XRCC1, TUBB3 and TS mRNA for Prediction of Response of Non-small Cell Lung Cancer to Chemoradiotherapy

Wu, Guoqiu; Liu, Nannan; Xue, Xiulei; Cai, Lei; Zhang, Chen; Qu, Qing-Rong; Yan, Xuejiao

doi:10.7314/apjcp.2014.15.10.4153

Cited by 10 publications

(8 citation statements)

References 25 publications

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“…ZEB2 is not the only miR-215 target dysregulated in NSCLC. Other functional targets of miR-215, including RB1 ( 35 ), TS ( 36 ), and ALCAM ( 37 , 38 ), also modulate NSCLC pathogenesis. Therefore, the potential regulatory circuitry affected by miR-215 is enormous, and the mechanisms underlying how miR-215 influences NSCLC progression require further clarification.…”

Section: Discussionmentioning

confidence: 99%

miR-215 functions as a tumor suppressor and directly targets ZEB2 in human non-small cell lung cancer

Hou

Zhen

et al. 2015

Oncology Letters

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MicroRNA-215 (miR-215) has previously been demonstrated to be dysregulated in a number of human malignancies and to be correlated with tumor progression. However, the expression and function of miR-215 in non-small cell lung cancer (NSCLC) has remained to be elucidated. Therefore, the present study aimed to investigate the effects of miR-215 in NSCLC tumorigenesis and development. Reverse transcription-quantitative polymerase chain reaction was used to evaluate miR-215 expression in NSCLC cell lines and primary tumor tissues. The association between miR-215 expression and certain clinicopathological factors was also determined, and the effects of miR-215 on the biological behavior of NSCLC cells were investigated. In addition, the potential regulatory function of miR-215 on zinc finger E-box-binding homeobox 2 (ZEB2) expression was examined. miR-215 expression was significantly downregulated in NSCLC cell lines and clinical specimens. Reduced miR-215 expression was significantly associated with lymph node metastasis and advanced TNM stage. Overexpression of miR-215 inhibited NSCLC cell proliferation, invasion and migration, and promoted cell apoptosis in vitro, and suppressed tumorigenicity in vivo. Furthermore, luciferase reporter assay analysis identified ZEB2 as a direct target of miR-215. These findings indicated that miR-215 may act as a tumor suppressor in NSCLC and may serve as a novel therapeutic agent for miR-based therapy.

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Section: Discussionmentioning

confidence: 99%

miR-215 functions as a tumor suppressor and directly targets ZEB2 in human non-small cell lung cancer

Hou

Zhen

et al. 2015

Oncology Letters

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“…In addition, TS mRNA is negatively connected with the clinical efficacy of pemetrexed (Zhao et al, 2014). TUBB3-coded β-Tubulin-Ⅲ (type Ⅲ β microtubulin) is an important part of cytoskeleton, a basic constructive unit of spindle during mitosis period, and a primary functional target of anti-microtubule agents, and tumors are sensitive to anti-microtubule chemotherapeutic agents when TUBB3 mRNA expression is low enough (Gan et al, 2007;Wu et al, 2014). STMN1-coded STMN1 protein has certain impact on the formation of spindle during mitosis period through improving the depolymerization of microtubule or inhibiting the synthesis of microtubule, so suppressing the expression of STMN1 protein can interfere the mitosis of malignant tumor cells so as to influence the proliferation and apoptosis of tumor cells.…”

Section: Discussionmentioning

confidence: 99%

Expression of TS, RRM1, ERCC1, TUBB3 and STMN1 Genes in Tissues of Non-small Cell Lung Cancer and its Significance in Guiding Postoperative Adjuvant Chemotherapy

Zou

Du²,

Sui³

et al. 2015

Asian Pacific Journal of Cancer Prevention

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Background: To explore the expression of TS, RRM, ERCC1, TUBB3 and STMN1 genes in the tissues of patients with non-small cell lung cancer (NSCLC) and its significance in guiding the postoperative adjuvant chemotherapy. Materials and Methods: Real time polymerase chain reaction (PCR) was applied to detect the expression of TS, RRM, ERCC1, TUBB3 and STMN1 genes in the tissues of NSCLC patients so as to analyze the relationship between the expression of each gene and the clinical characteristics and to guide the postoperative individualized chemotherapy according to the detection results of NSCLC patients. Results: Expression of TS gene was evidently higher in patients with adenocarcinoma than those with non-adenocarcinoma (P=0.013) and so was the expression of ERCC1 (P=0.003). The expression of TUBB3 gene was obviously higher in NSCLC patients in phases Ⅰ/Ⅱ and Ⅳ than those in phase Ⅲ (P 1 =0.021; P 2 =0.004), and it was also markedly higher in patients without lymph node metastasis than those with (P=0.008). The expression of STMN1 gene was apparently higher in patients in phase Ⅰ/Ⅱ than those in phase Ⅳ (P=0.002). There was no significant difference between the rest gene expression and the clinical characteristics of NSCLC patients (P>0.05). Additionally, the diseasefree survival (DFS) was significantly longer in patients receiving gene detections than those without (P=0.021). Conclusions: The selection of chemotherapeutic protocols based singly on patients' clinical characteristics has certain blindness. However, the detection of tumor-susceptible genes can guide the postoperative adjuvant chemotherapy and prolong the DFS of NSCLC patients.

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“…Microtubules are promising targets for chemotherapeutic drugs aimed at disturbing mitosis and inducing cell death in frequently dividing tumor cells (Wu et al, 2014). EpoB, due to its high cytotoxicity in ovarian cancer cells, seems to be a very promising alternative to the current strategy of ovarian cancer treatment, currently in which PTX is the main component (Unal et al, 2014;Vahdat et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Nuclear DNA Damage and Repair in Normal Ovarian Cells Caused by Epothilone B

Rogalska

Marczak²

2015

Asian Pacific Journal of Cancer Prevention

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This study was designed to assess, whether a new chemotherapeutic microtubule inhibitor, Epothilone B (EpoB, Patupilone), can induce DNA damage in normal ovarian cells (MM14.Ov), and to evaluate if such damage could be repaired. The changes were compared with the effect of paclitaxel (PTX) commonly employed in the clinic. The alkaline comet assay technique and TUNEL assay were used. The kinetics of DNA damage formation and the level of apoptotic cells were determined after treatment with IC50 concentrations of EpoB and PTX. It was observed that PTX generated significantly higher apoptotic and genotoxic changes than EpoB. The peak was observed after 48 h of treatment when the DNA damage had a maximal level. The DNA damage induced by both tested drugs was almost completely repaired. As EpoB in normal cells causes less damage to DNA it might be a promising anticancer drug with potential for the treatment of ovarian tumors.

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Multiplex Real-time PCR for RRM1, XRCC1, TUBB3 and TS mRNA for Prediction of Response of Non-small Cell Lung Cancer to Chemoradiotherapy

Cited by 10 publications

References 25 publications

miR-215 functions as a tumor suppressor and directly targets ZEB2 in human non-small cell lung cancer

miR-215 functions as a tumor suppressor and directly targets ZEB2 in human non-small cell lung cancer

Expression of TS, RRM1, ERCC1, TUBB3 and STMN1 Genes in Tissues of Non-small Cell Lung Cancer and its Significance in Guiding Postoperative Adjuvant Chemotherapy

Nuclear DNA Damage and Repair in Normal Ovarian Cells Caused by Epothilone B

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