Multiplex real-time PCR for the identification and quantification of DNA from duck, pig and chicken in Chinese blood curds

Cheng, X.; He, Wen; Huang, Feng Ting; Huang, Ming; Zhou, G.F.

doi:10.1016/j.foodres.2014.01.047

Cited by 58 publications

(25 citation statements)

References 41 publications

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“…The same authors also reported that immunoassays have been widely used to validate meat authenticity, albeit with limitations regarding cross-reactions associated with antibody nonspecificity and protein structural changes. CHENG et al (2014) stated that DNA-based methods are used more frequently, especially due tothe stability and ubiquity of DNA in tissues. Polymerase chain reaction (PCR) is an efficient method for detecting adulteration, and this method has already been usedto detect adulteration in meat products (DAGUER et al, 2010;OLIVEIRA et al, 2015;KARABASANAVAR et al, 2013;ALI et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Brazilian ground beef authentication by multiplex polymerase chain reaction

Oliveira

Pedroso²,

Cardilli³

et al. 2018

Cienc. Rural

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The aim of the present study was to assess the efficacy ofmultiplex PCR in detecting the adulterationof commercially available ground beefvia addition and/orsubstitution ofground buffalo meat. Experimentally adulterated ground beefsamples were prepared in triplicate, and dilutions of DNA from Bos taurus and Bubalusbubalis were prepared to determine the detection limit of the method. Concurrently, 91 ground meatsamples sold as “ground beef” were collected from differentstores in northern Brazil andanalyzed bymultiplex PCR. Buffalo DNA was detected in 17.5% of the collected ground meat samples.Our results showed that multiplex PCR is an efficient method for detectingthe incorporation of groundbuffalo meatatpercentages ranging from 10 to 100% and the incorporation of beef at percentages ranging from0.1 to 100% intoground meat samples.

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Section: Introductionmentioning

confidence: 99%

Brazilian ground beef authentication by multiplex polymerase chain reaction

Oliveira

Pedroso²,

Cardilli³

et al. 2018

Cienc. Rural

View full text Add to dashboard Cite

show abstract

“…Conventional PCR techniques allow qualitative and speciesspecific detection in food and food ingredients (Rodriguez (Dalmasso et al 2004;Safdar et al 2014;Chen et al 2015). Real-time PCR has been widely used for the quantitative determination of animal species but it is not cost-effective for use to simply detect food fraud (Dooley et al 2004;Cheng et al 2014). Mitochondrial DNA (mtDNA) is used as target gene for species-specific detection because it has a simple molecular structure and has a rapid evolution rate (Wolf et al1999;Girish et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Multiplex‐PCR Assay for Simultaneous Identification of Lamb, Beef and Duck in Raw and Heat‐Treated Meat Mixtures

Qin

Hong

Kim³

2015

Journal of Food Safety

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A multiplex PCR technique was applied for the simultaneous identification of duck, beef and lamb meats in meat mixtures. To simulate food adulteration, lamb and duck meat (10-0.5% each) were mixed into beef (80-99%) and beef and duck meat (10-0.5% each) were mixed into lamb (80-99%). Species-specific primer sets were designed from the cytochrome b gene of mitochondrial DNA. A universal 18S rRNA primer set was also applied as a positive control in the PCR reaction. The sizes of the PCR products for eukaryotes, lamb, beef and duck were 99, 133, 166 and 204 bp, respectively. All primer sets showed specificity in the PCR reactions using template DNAs from 16 animal species. The sensitivity for both single and multiplex PCR was 0.005 ng. The detection limit for target species in raw and heat-treated (100, 121 and 200C for 20 min) meat mixtures was 1%. This multiplex PCR method successfully discriminated raw and cooked lamb, beef and duck meat samples.

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“…Chen, Weiss & Shahidi (2006) ;Su, Wu, Liu, Qu, Liu, Chen, Huang & Hong (2007), portrayed the advancements in nanotechnology for development of targeted and smart delivery systems as multifunctional nanoscale nutraceutical delivery systems that do not only have the ability to detect and recognize appropriate location, but also analyze local and global needs as well as decide whether or how much payload should be released (Figure 1). Consequently, smart delivery systems would monitor the response of nanoscale nutraceutical delivery systems for feedback (Scrinis & Lyons, 2007;Ravichandran, 2010).…”

Section: Smart Delivery Systemsmentioning

confidence: 99%

Nanotechnologies associated to floral resources in agri-food sector

Ammar¹

2018

Acta Agron.

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Nanotechnology advent in agri-food sector is set to prompt next revolution in agricultural engineering. However, there is a perpetually rising need for development of new nanotechnologies that could synchronically work with various agrochemicals such as fertilizers, pesticides, herbicides, and growth promoters to potentially increase farmlands efficiency, preserve agro-ecosystems, and diminish the negative health risks imposed by conventional practices. In nanotechnology, smart delivery systems that utilize either nanoscale carriers such as clay nanotubes and carbon nanotubes or nanoparticles such as mesoporous silica nanoparticles and silver nanoparticles, could enable not only the accurate and targeted delivery of functional ingredients but also their impartial dissemination over farmlands. Nanotechnology has found applications for bioremediation of irrigation water and agricultural runoff, crop breeding, agronomic traits via genetic manipulation of genomes at molecular level, and detection of minute quantities of contaminants and stressors as well as early detection of plant diseases and continuous monitoring of plant environment through employment of nano-biosensors. Scientists are diligently working to explore new substitutes for conventional technologies. Advancements in nanotechnology could help them to explore new frontiers and find novel applications in agri-food sector.Keywords: Agrochemicals; bioremediation; crop breeding; micro-fabricated xylem vessels; smart delivery systems; smart nano-dust. ResumenEl advenimiento de la nanotecnología en el sector agrícola está programado para impulsar la próxima revolución en la ingeniería agrícola. Sin embargo, existe una creciente necesidad de desarrollo de nuevas nanotecnologías que puedan trabajar sincrónicamente con diversos agroquímicos como fertilizantes, pesticidas, herbicidas y promotores de crecimiento para aumentar potencialmente la eficiencia de las tierras de cultivo, preservar los agroecosistemas y disminuir lo negativo Riesgos para la salud impuestas por las prácticas convencionales. En nanotecnología, los sistemas inteligentes de entrega que utilizan portadores a nanoescala como nanotubos de arcilla y nanotubos de carbono o nanopartículas como nanopartículas de sílice mesoporosas y nanopartículas de plata, podrían permitir no sólo la entrega precisa y específica de ingredientes funcionales sino también su difusión imparcial sobre las tierras de cultivo. La nanotecnología ha encontrado aplicaciones para la biorremediación del agua de riego y la escorrentía agrícola, la mejora de los rasgos de los cultivos mediante la manipulación genética de los genomas a nivel molecular y la detección de cantidades diminutas de contaminantes y factores de estrés, El seguimiento continuo del medio ambiente vegetal mediante el empleo de nanobiosensores. Los científicos están trabajando diligentemente para explorar nuevos sustitutos de las tecnologías convencionales. Los avances en nanotecnología podrían ayudarles a explorar nuevas fronteras y encontrar nuevas...

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Multiplex real-time PCR for the identification and quantification of DNA from duck, pig and chicken in Chinese blood curds

Cited by 58 publications

References 41 publications

Brazilian ground beef authentication by multiplex polymerase chain reaction

Brazilian ground beef authentication by multiplex polymerase chain reaction

Multiplex‐PCR Assay for Simultaneous Identification of Lamb, Beef and Duck in Raw and Heat‐Treated Meat Mixtures

Nanotechnologies associated to floral resources in agri-food sector

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