Multiplex Sequence-Tagged Site PCR for Efficient Screening of Microdeletions in Y Chromosome in Infertile Males with Azoospermia or Severe Oligozoospermia

Complex chromosomal rearrangements in infertile males: complexity of rearrangement affects spermatogenesis

“…Genomic DNAs were extracted from peripheral blood using the QIAamp DNA blood Midi kit (Qiagen, Hilden, Germany). The DAZ gene family (DAZ1, 2, 3, and 4) and sequences-tagged sites in the AZF regions (AZFb, AZFc: sY117, sY127, sY143, sY134, sY138, sY152, sY153, sY147, sY149, sY269, sY157, and sY158) were analyzed by polymerase chain reaction (PCR), performed as described elsewhere (10,11). The PCR products were electrophoresed on 2% agarose gels and visualized under ultraviolet light.…”

Two Males with SRY-Positive 46,XX Testicular Disorder of Sex Development

“…In addition to a negative control, normal male DNA and normal female DNA were used as controls. SRY (sY14 and sY1532), ZFY, sY84, sY86, sY127, sY134, sY254, sY255, SMCY, and CDY2 locus amplification was performed using multiplex PCR (Simoni et al 2004;Nakashima et al 2002). The amplification products were resolved on 2% agarose and analyzed using a video gel documentation system (Biolabs, Kyoto, Japan) after staining with ethidium bromide.…”

Evaluation of Sperm Chromatin Quality and Screening of Y Chromosome Microdeletions in Greek Males with Severe Oligozoospermia

“…DNA was extracted from peripheral blood granulocytes using the Puregene Kit for Genomic DNA purification. A set of 8 Y-specific sequence-tagged site (STS) primers was used amplifying STSs in the AZFa, AZFb, and AZFc regions [17] which represent more than 90% of all Y microdeletions [20,21]. PCR was performed [21].…”