“…All viral RNA samples were stored at −80˚C until required for experiments. Premixed DP-LAMP buffer consisted of 12.5 μL of WarmStart 2X MM (NEB, MA, USA), 2.5 μL of WNV or DENV-I 10 X DP-LAMP primers and probes (1.6 μM of FIP and BIP, 0.4 μM of LB for WNV or 0.5 μM of LB for DENV, 0.5 μM of LF for WNV or 0.4 μM of LF for DENV-I, 0.2 μM of F3, and B3, 0.1 μM of fluorescent FAM-3 0 labeled probe and 0.15 μM of Iowa Black FQ-5 0 labeled LB/LF probe), 0.5 μL of RNase Inhibitor (NEB, MA, USA), 0.5 μL of Antarctic UDG (NEB, MA, USA), 0.25 μL of ET SSB (NEB, MA, USA), and 0.2 μL of dUTP (Promega, WI, USA) [21,24,30]. An aliquot of 7.55 μL of aqueous sucrose was added to the pre-mixed DP-LAMP buffer to achieve specified concentrations, which included 0%, 5%, 10%, 20%, and 40%.…”