Multiplexed Fluorescence Detection for DNA Sequencing

Zhu, Li; Soper, Steven A.

doi:10.1007/0-387-33016-x_23

Cited by 3 publications

(2 citation statements)

References 95 publications

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“…Noble metal NPs are nowadays used for DNA sequencing, replacing the dyes based technology [71,72]. The use of dyes causes some problems as photobleaching and instability in certain environments that limit their applications [72,73]. Moreover, the spatial resolution and sensitivity required to carry out thousands of test compel to use confocal microscopy leading to complex and expensive devices [74].…”

Section: Dna Sequencingmentioning

confidence: 99%

Surface plasmons in metallic nanoparticles: fundamentals and applications

García¹

2011

J. Phys. D: Appl. Phys.

773

424

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The excitation of surface plasmons in metallic nanoparticles induces optical properties hardly achievable in other optical materials, yielding a wide range of applications in many fields. This review presents an overview of surface plasmons in metallic nanoparticles. The concept of surface plasmons in nanoparticles is qualitatively described using a comparison with simple linear oscillators. The mathematical models to carry on calculations on surface plasmons are presented as well as the most common approximations. The different parameters governing the features of surface plasmons and their effect on the optical properties of the materials are reviewed. Finally, applications of surface plasmons in different fields as biomedicine, energy, environment protection and information technology are revised.

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Section: Dna Sequencingmentioning

confidence: 99%

Surface plasmons in metallic nanoparticles: fundamentals and applications

García¹

2011

J. Phys. D: Appl. Phys.

773

424

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“…Here we present a rational design of fluorescent labels that allows tuning of fluorescent outputs through a wide emission window via cassettes composed of donor and acceptor chromophores. ,,, Specifically, this paper describes cassettes that work via excitation of a BODIPY-based − donor with blue light (e.g., 488 nm) followed by fast energy transfer (ET) to variable cyanine-based , acceptors, which then emit red light. Three cassettes were prepared, 1a − 1c (Scheme ).…”

Section: Introductionmentioning

confidence: 99%

Encapsulated Energy-Transfer Cassettes with Extremely Well Resolved Fluorescent Outputs

et al. 2010

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This paper concerns the development of water-compatible fluorescent imaging-probes with tunable photonic properties that can be excited at a single wavelength. Bichromophoric cassettes 1a -1c consisting of a BODIPY donor and a cyanine acceptor were prepared using a simple synthetic route, and their photophysical properties were investigated. Upon excitation of the BODIPY moiety at 488 nm the excitation energy is transferred through an acetylene bridge to the cyanine dye acceptor, which emits light at approximately 600, 700, and 800 nm, ie with remarkable dispersions. This effect is facilitated by efficient energy transfer that gives a 'quasiStokes' shift of between 86 -290 nm opening a huge spectral window for imaging. The emissive properties of the cassettes depend on the energy transfer (ET) mechanism: the faster the transfer, the more efficient it is. Measurements of rates of energy transfer indicate that a through-bond energy transfer takes place in the cassettes 1a and 1b that is two orders of magnitude faster than the classical through-space, Förster, energy transfer (in the case of cassette 1c, however, both mechanisms are possible, and the rate measurements do not allow us to discern between them). Thus the cassettes 1a -1c are well suited for multiplexing experiments in biotechnological methods that involve a single laser-excitation source. However, for widespread application of these probes their solubility in aqueous media must be improved. Consequently, the probes were encapsulated in calcium phosphate/silicate nanoparticles (diameter ca 22 nm) that are freely dispersible in water. This encapsulation process resulted in only minor changes in the photophysical properties of the cassettes. The system based on cassette 1a was chosen to probe how effectively these nanoparticles could be used to deliver the dyes into cells. Encapsulated cassette 1a permeated Clone 9 rat liver cells where it localized in the mitochondria and fluoresced through the acceptor part, ie red. Overall, this paper reports readily accessible, cyanine-based through-bond energy transfer cassettes that are lypophilic but can be encapsulated to form nanoparticles that disperse freely in water. These particles can be used to enter cells and to label organelles.

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6‐Carboxyfluorescein

2015

Handbook of Fluorescent Dyes and Probes

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Multiplexed Fluorescence Detection for DNA Sequencing

Cited by 3 publications

References 95 publications

Surface plasmons in metallic nanoparticles: fundamentals and applications

Surface plasmons in metallic nanoparticles: fundamentals and applications

Encapsulated Energy-Transfer Cassettes with Extremely Well Resolved Fluorescent Outputs

6‐Carboxyfluorescein

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