2018
DOI: 10.1016/j.ab.2018.01.005
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Multiplexed isothermal nucleic acid amplification

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Cited by 83 publications
(61 citation statements)
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“…have proved reliable application for this purpose. Nevertheless, SDA has poorly achieved longer sequence amplification and a comparable amplification as for LAMP toward turbidity measurement, but under stringent conditions, possesses higher sensitivity, specificity, and cost-efficiency, and can be multiplexed and reprogrammed for various targets [58,[93][94][95][96]. But also, other isothermal amplification methods such as LAMP have hardly shown multiplexing capacity [97][98][99] and are solely dye dependent for colorimetric detection and quantification, which are target independent and nonspecific.…”
Section: Discussion Conclusion and Future Perspectivesmentioning
confidence: 99%
“…have proved reliable application for this purpose. Nevertheless, SDA has poorly achieved longer sequence amplification and a comparable amplification as for LAMP toward turbidity measurement, but under stringent conditions, possesses higher sensitivity, specificity, and cost-efficiency, and can be multiplexed and reprogrammed for various targets [58,[93][94][95][96]. But also, other isothermal amplification methods such as LAMP have hardly shown multiplexing capacity [97][98][99] and are solely dye dependent for colorimetric detection and quantification, which are target independent and nonspecific.…”
Section: Discussion Conclusion and Future Perspectivesmentioning
confidence: 99%
“…Since this first report, the application of LAMP has been increasingly used and adopted as an alternative method to those based on PCR. LAMP, in fact, is continuously being implemented in the medicine, agriculture, and food industries, with approaches that include the screening of viral and bacterial strain mutations, analysis of fungicide resistant mutations, analysis of micro RNAs, herbal medicine identification, plant pathogen vectors identification, single nucleotide polymorphisms analysis, and detection of genetically modified organisms [16][17][18][19][20]. The reasons for the development of the LAMP methodology were founded on the attempt to overcome some drawbacks of the conventional PCR, a method that requires the acquisition of a high-cost equipment known as thermal cycler.…”
Section: Loop Mediated Isothermal Amplification (Lamp)mentioning
confidence: 99%
“…Such sequence-specic detection enables the accurate identication of amplicons without being affected by unspecic products. Moreover, sequence-specic detection allows for differentiation between and identication of multiple targets simultaneously in one assay (one-pot multiplexing 43 ). Appropriate selection and adaption of biorecognition elements plays an essential role in biosensing performance, especially with respect to sensitivity and speci-city.…”
Section: Introductionmentioning
confidence: 99%
“…50,51 Methods for real-time monitoring and endpoint detection of LAMP were summarized in a brief review by Zhang et al 52 These methods comprise diverse sequence-independent and sequence-specic methods. Furthermore, Mayboroda et al 43 reviewed multiplex methods for isothermal amplication in general. As a completion of the previous work and in consideration of the novel methods published aer 2014, we provide the rst comprehensive, systematic classication and critical analysis of such methods.…”
Section: Introductionmentioning
confidence: 99%