Multiplexed lateral flow immunoassay to discriminate Solenopsis invicta, Solenopsis richteri, and Solenopsis invicta × richteri hybrids

Valles, Steven M.; Strong, C. A.; Callcott, A. M.

doi:10.1007/s00040-018-0637-4

Cited by 3 publications

(3 citation statements)

References 22 publications

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“…Lateral flow immunoassay is another IFA assessment technique that has been developed to facilitate rapid and portable field detection of RIFA, BIFA, and HIFA ( Valles et al 2016 , 2018 ). The commercialized InvictDetect ImmunoStrip (Agdia, Inc., Elkhart, IN) reacts with worker ant proteins from RIFA colonies or HIFA colonies near RIFA, but it cannot be used to determine the colony indices or if the ants are HIFA or RIFA.…”

Section: Discussionmentioning

confidence: 99%

“…The commercialized InvictDetect ImmunoStrip (Agdia, Inc., Elkhart, IN) reacts with worker ant proteins from RIFA colonies or HIFA colonies near RIFA, but it cannot be used to determine the colony indices or if the ants are HIFA or RIFA. Likewise, the noncommercialized multiplexed lateral flow immunoassay detects proteins associated with RIFA, BIFA, or HIFA but depending on protein amounts, HIFA close to RIFA or HIFA close to BIFA may not be distinguished from the parent species ( Valles et al 2018 ). Thus, a colorimetric test to rapidly recognize BIFA, HIFA, or RIFA, as well as to estimate the approximate HIFA index range, could be useful for some research and field applications where general taxonomic identity of IFA is needed.…”

Section: Discussionmentioning

confidence: 99%

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Relationship of Imported Fire Ant (Hymenoptera: Formicidae) Integument Coloration to Cuticular Hydrocarbon and Venom Alkaloid Profiles

Pandey

Addesso

Alexander

et al. 2021

Environmental Entomology

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Red imported fire ant (Solenopsis invicta Buren; RIFA) and black imported fire ant (Solenopsis richteri Forel (Hymenoptera: Formicidae); BIFA) are considered distinct species with introgression via a reproductively functional hybrid (HIFA). The RIFA and BIFA common names are based on relative coloration. Due to human color perception variation, using color to identify RIFA, BIFA or HIFA is challenging. Fire ant identification traditionally involves molecular or chemical techniques, but a colorimetric test could allow rapid and low-cost identification. In this study, ant integument coloration was measured by spectrophotometer, and color attributes were compared to a combined gas chromatography (GC) index derived from cuticular hydrocarbon and venom alkaloid indices. Significant Pearson Correlation coefficients were found for colony GC index versus color attributes red to green (a*), blue to yellow (b*), chroma (C*), and hue (h*), but not lightness (L*). The RIFA colonies were distinct from BIFA for four of five color attributes and plots of the a*b* and C*h* horizontal axis of the L*a*b* and L*C*h* color spaces. Color attributes for HIFA indices were not distinct from BIFA and RIFA parental species, but HIFA a*b* and C*h* plots were distinct from RIFA and a*b* plots from BIFA. Color attributes a*, b*, and C* increased and h* decreased with GC index in a sinusoidal pattern. In conclusion, most RIFA and BIFA color attributes were distinct and a*b* and C*h* color axes plots had potential to distinguish HIFA from parental species, but coloration of HIFA indices was variable and complicated identifications among HIFA phenotypes, RIFA and BIFA.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Relationship of Imported Fire Ant (Hymenoptera: Formicidae) Integument Coloration to Cuticular Hydrocarbon and Venom Alkaloid Profiles

Pandey

Addesso

Alexander

et al. 2021

Environmental Entomology

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“…One antibody captures the Soli2 venom protein from an ant homogenate, and the other, at a different position on the venom protein, reports its presence. The LFA was found to be species-specific against U.S. and Australian species of ants [10,12]. The technology was later commercialized as InvictDetect TM Immunostrip ® (Agdia, Elkhart, IN) and is now available for purchase by the public (https://orders.agdia.com/invictdetectisk-49700-0010).…”

Section: Introductionmentioning

confidence: 99%

Efficacy of the InvictDetectTM Immunostrip® to Taxonomically Identify the Red Imported Fire Ant, Solenopsis invicta, Using A Single Worker Ant

Valles

Strong

Emmitt

et al. 2020

Insects

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The early detection and identification of the red imported fire ant Solenopsis invicta are crucial to intercepting and preventing it from becoming established in new areas. Unfortunately, the visual identification of fire ants to species is difficult and ant samples must often be couriered to an expert for positive identification, which can delay control interventions. A lateral flow immunoassay that provides a rapid and portable method for the identification of S. invicta ants was developed and commercialized, and it is available from Agdia, Inc. under the trade name InvictDetectTM. While the test was 100% accurate when using the recommended minimum sample of three ant workers, InvictDetectTM was field tested for the first time while using homogenates prepared from single S. invicta workers to determine the effectiveness of the method under these non-recommended conditions. Disregarding social form, the false negative rate was 25.5% for an initial single worker ant test and 10% after a repeat test was performed. The InvictDetectTM false negative response was independent of worker weight. Though InvictDetectTM requires a minimum of three worker ants, the test improves upon current identification methods because it can be conducted in the field, be completed in 10–30 min, and requires no special training or expertise.

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Unique venom proteins from Solenopsis invicta x Solenopsis richteri hybrid fire ants

et al. 2021

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Multiplexed lateral flow immunoassay to discriminate Solenopsis invicta, Solenopsis richteri, and Solenopsis invicta × richteri hybrids

Cited by 3 publications

References 22 publications

Relationship of Imported Fire Ant (Hymenoptera: Formicidae) Integument Coloration to Cuticular Hydrocarbon and Venom Alkaloid Profiles

Relationship of Imported Fire Ant (Hymenoptera: Formicidae) Integument Coloration to Cuticular Hydrocarbon and Venom Alkaloid Profiles

Efficacy of the InvictDetectTM Immunostrip® to Taxonomically Identify the Red Imported Fire Ant, Solenopsis invicta, Using A Single Worker Ant

Unique venom proteins from Solenopsis invicta x Solenopsis richteri hybrid fire ants

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