Multiplexed redox gating measurements with a microelectrospotter. Towards electrochemical readout of molecularly imprinted polymer microarrays

Supala, Eszter; Tamás, Luca; Erdőssy, Júlia; Gyurcsányi, Róbert E.

doi:10.1016/j.elecom.2020.106812

Cited by 7 publications

(3 citation statements)

References 16 publications

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“… 30 To address this demand, we introduced recently microelectrospotting 34 for the electrosynthesis of surface-imprinted protein MIP-arrays. The procedure involves an electrochemical spotting pin 35 for localized electropolymerization of monomer-template protein mixtures and enabled multiplexed screening of a large number of synthetic conditions for protein MIPs as well as their affinity. However, it is not suited for oriented epitope imprinting.…”

Section: Introductionmentioning

confidence: 99%

Peptide epitope-imprinted polymer microarrays for selective protein recognition. Application for SARS-CoV-2 RBD protein

et al. 2022

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Section: Introductionmentioning

confidence: 99%

Peptide epitope-imprinted polymer microarrays for selective protein recognition. Application for SARS-CoV-2 RBD protein

et al. 2022

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“…In addition, the method matches the thrifty consumption of chemicals, which is especially advantageous in the case of protein templates. Moreover, microelectrospotting can also offer a means for multiplexed readout of MIP arrays, providing the possibility for rapid screening [147].…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Electrochemically Synthesized MIP Sensors: Applications in Healthcare Diagnostics

Ayankojo,

Reut,

Syritski

2024

Biosensors

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Early-stage detection and diagnosis of diseases is essential to the prompt commencement of treatment regimens, curbing the spread of the disease, and improving human health. Thus, the accurate detection of disease biomarkers through the development of robust, sensitive, and selective diagnostic tools has remained cutting-edge scientific research for decades. Due to their merits of being selective, stable, simple, and having a low preparation cost, molecularly imprinted polymers (MIPs) are increasingly becoming artificial substitutes for natural receptors in the design of state-of-the-art sensing devices. While there are different MIP preparation approaches, electrochemical synthesis presents a unique and outstanding method for chemical sensing applications, allowing the direct formation of the polymer on the transducer as well as simplicity in tuning the film properties, thus accelerating the trend in the design of commercial MIP-based sensors. This review evaluates recent achievements in the applications of electrosynthesized MIP sensors for clinical analysis of disease biomarkers, identifying major trends and highlighting interesting perspectives on the realization of commercial MIP-endowed testing devices for rapid determination of prevailing diseases.

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“…Scopoletin is a coumarin derivative (7-hydroxy-6-methoxy-coumarin) that was first used for the immobilization of biomolecules by electropolymerization [36] and emerged ultimately as a generic electropolymerizable monomer for the preparation of protein MIPs [19,31,33,[37][38]. However, the generic use is not associated in all instances with a sufficiently high affinity and selectivity to the target for routine applications.…”

Section: Synthesis Of Asp-umbelliferonementioning

confidence: 99%

Novel functional monomer for the electrochemical synthesis of highly affine epitope‐imprinted polymers

et al. 2023

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To address the lack of functional monomer diversity for the electrosynthesis of protein‐selective molecularly imprinted polymers (MIPs), we introduce a new concept able to lead to a new class of functional monomers. This is based on conjugating an electropolymerizable monomer unit (umbelliferone) to an amino acid for closer mimicking of protein‐based natural affinity ligands such as antibodies. As the first representative of this class of monomers an aspartate‐umbelliferone (Asp‐UMB) conjugate was synthesized and here we provide the proof for its suitability to generate highly affine MIPs for proteins by epitope imprinting. As model we used a heptapeptide (GFNCYFP) stemming from the receptor binding domain (RBD) of the SARS‐CoV‐2 spike protein to generate epitope‐imprinted polymers able to recognize the parent RBD protein. For rapid optimization and assessment of the binding kinetics we prepared MIP microarrays on surface plasmon resonance imaging (SPRi) chips. First the peptides were microspotted on the bare gold surface of the chips followed by the electropolymerization of Asp‐UMB. This resulted in ca. 2 nm thick, highly uniform, and electrically insulating polymer film, well suited both for hierarchical epitope imprinting and SPRi read‐out. Taking advantage also of the on‐chip optimization enabled by the microarray format the increased functional diversity of the new monomer resulted in highly affine MIPs with equilibrium dissociation constants in the lower picomolar range.

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Multiplexed redox gating measurements with a microelectrospotter. Towards electrochemical readout of molecularly imprinted polymer microarrays

Cited by 7 publications

References 16 publications

Peptide epitope-imprinted polymer microarrays for selective protein recognition. Application for SARS-CoV-2 RBD protein

Peptide epitope-imprinted polymer microarrays for selective protein recognition. Application for SARS-CoV-2 RBD protein

Electrochemically Synthesized MIP Sensors: Applications in Healthcare Diagnostics

Novel functional monomer for the electrochemical synthesis of highly affine epitope‐imprinted polymers

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