Multiplexed suspension array platform for high-throughput protein assays

Birtwell, S.; Broder, Graham R.; Roach, Peter L.; Morgan, Hywel

doi:10.1007/s10544-012-9641-z

Cited by 8 publications

(7 citation statements)

References 28 publications

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“…2). 30 A sufficient number of accurate barcodes are critical to fulfill the high density analysis, and various encoding schemes have been used to this end, including spectrometric encoding, 12,15,18,30,31 graphical encoding, 19,[32][33][34][35] chemical encoding, [36][37][38][39][40] electronic encoding, 41,42 physical encoding, 43,44 and magnetic encoding. 45,46 Furthermore, the combination of different encoding techniques can produce an even larger amount of barcodes.…”

Section: Why Do Suspension Arrays Based On Nanoparticleencoded Micros...mentioning

confidence: 99%

Suspension arrays based on nanoparticle-encoded microspheres for high-throughput multiplexed detection

et al. 2015

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Spectrometrically or optically encoded microsphere based suspension array technology (SAT) is applicable to the high-throughput, simultaneous detection of multiple analytes within a small, single sample volume. Thanks to the rapid development of nanotechnology, tremendous progress has been made in the multiplexed detecting capability, sensitivity, and photostability of suspension arrays. In this review, we first focus on the current stock of nanoparticle-based barcodes as well as the manufacturing technologies required for their production. We then move on to discuss all existing barcode-based bioanalysis patterns, including the various labels used in suspension arrays, label-free platforms, signal amplification methods, and fluorescence resonance energy transfer (FRET)-based platforms. We then introduce automatic platforms for suspension arrays that use superparamagnetic nanoparticle-based microspheres. Finally, we summarize the current challenges and their proposed solutions, which are centered on improving encoding capacities, alternative probe possibilities, nonspecificity suppression, directional immobilization, and “point of care” platforms. Throughout this review, we aim to provide a comprehensive guide for the design of suspension arrays, with the goal of improving their performance in areas such as multiplexing capacity, throughput, sensitivity, and cost effectiveness. We hope that our summary on the state-of-the-art development of these arrays, our commentary on future challenges, and some proposed avenues for further advances will help drive the development of suspension array technology and its related fields.

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Section: Why Do Suspension Arrays Based On Nanoparticleencoded Micros...mentioning

confidence: 99%

Suspension arrays based on nanoparticle-encoded microspheres for high-throughput multiplexed detection

et al. 2015

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“…As for the majority of bead-based assays, reading the code and quantifying the target reporter signal on hydrogel particles can often rely on optical detection methods (for general reviews on bead-based array decoding see [83, 112, 116, 117]). Two approaches have been reported for signal acquisition: imaging methods [33, 34, 37, 38] and flow cytometry-like scanning techniques [44, 69].…”

Section: Processing Particles and Reading The Codementioning

confidence: 99%

Hydrogel microparticles for biosensing

Goff

Srinivas

Hill³

et al. 2015

European Polymer Journal

180

144

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Due to their hydrophilic, biocompatible, and highly tunable nature, hydrogel materials have attracted strong interest in the recent years for numerous biotechnological applications. In particular, their solution-like environment and non-fouling nature in complex biological samples render hydrogels as ideal substrates for biosensing applications. Hydrogel coatings, and later, gel dot surface microarrays, were successfully used in sensitive nucleic acid assays and immunoassays. More recently, new microfabrication techniques for synthesizing encoded particles from hydrogel materials have enabled the development of hydrogel-based suspension arrays. Lithography processes and droplet-based microfluidic techniques enable generation of libraries of particles with unique spectral or graphical codes, for multiplexed sensing in biological samples. In this review, we discuss the key questions arising when designing hydrogel particles dedicated to biosensing. How can the hydrogel material be engineered in order to tune its properties and immobilize bioprobes inside? What are the strategies to fabricate and encode gel particles, and how can particles be processed and decoded after the assay? Finally, we review the bioassays reported so far in the literature that have used hydrogel particle arrays and give an outlook of further developments of the field.

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“…Furthermore, the noncovalent attachment of the cholesteryl oligonucleotide has also been reported. 27 In addition, SU-8-encoded microparticles are chemically modied to perform multiplexed assays 16,28 or to be used for solid-phase synthesis. 24,25,29,30 Recently, a swelling-deswelling method for the surface modication of the 2D and 3D patterns of SU-8 was used, and demonstrated oligonucleotide attachment.…”

Section: Introductionmentioning

confidence: 99%