Multiporous Cellulose Microcarrier for the Development of a Hybrid Artificial Liver Using Isolated Hepatocytes

Kino, Yasuhisa; Sawa, Masayuki; Kasai, S.; Mito, Michio

doi:10.1006/jsre.1998.5389

Cited by 46 publications

(22 citation statements)

References 8 publications

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“…A material composed of porous plant cellulose has been shown to be biocompatible with bone tissue and hepatocytes. 9,20 Research conducted on an implanted cellulose sponge showed that it can be regarded as a slowly degradable material. 9 As mentioned by the same authors, this material can be considered nondegradable if used as a temporary wound coverage for a short period of time.…”

Section: The Significant Biomedical Potential Of Microbial Cellulose mentioning

confidence: 99%

The Future Prospects of Microbial Cellulose in Biomedical Applications

et al. 2006

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Microbial cellulose has proven to be a remarkably versatile biomaterial and can be used in wide variety of applied scientific endeavors, such as paper products, electronics, acoustics, and biomedical devices. In fact, biomedical devices recently have gained a significant amount of attention because of an increased interest in tissue-engineered products for both wound care and the regeneration of damaged or diseased organs. Due to its unique nanostructure and properties, microbial cellulose is a natural candidate for numerous medical and tissue-engineered applications. For example, a microbial cellulose membrane has been successfully used as a wound-healing device for severely damaged skin and as a small-diameter blood vessel replacement. The nonwoven ribbons of microbial cellulose microfibrils closely resemble the structure of native extracellullar matrices, suggesting that it could function as a scaffold for the production of many tissue-engineered constructs. In addition, microbial cellulose membranes, having a unique nanostructure, could have many other uses in wound healing and regenerative medicine, such as guided tissue regeneration (GTR), periodontal treatments, or as a replacement for dura mater (a membrane that surrounds brain tissue). In effect, microbial cellulose could function as a scaffold material for the regeneration of a wide variety of tissues, showing that it could eventually become an excellent platform technology for medicine. If microbial cellulose can be successfully mass produced, it will eventually become a vital biomaterial and will be used in the creation of a wide variety of medical devices and consumer products.

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Section: The Significant Biomedical Potential Of Microbial Cellulose mentioning

confidence: 99%

The Future Prospects of Microbial Cellulose in Biomedical Applications

et al. 2006

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“…15 Studies show that porous RC is biocompatible. 16,17 It has been investigated as an implant in orthopedic surgery 18 and as a sealing material for the femoral component in hip prostheses. 19 Martson et al 15,18 observed osseous tissue ingrowth and the regeneration of cartilage following the use of RC sponge as an implant.…”

Section: Introductionmentioning

confidence: 99%

Fabrication and evaluation of porous 2,3‐dialdehydecellulose membrane as a potential biodegradable tissue‐engineering scaffold

RoyChowdhury

Kumar

2005

J Biomedical Materials Res

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A simple, novel method to produce porous 2,3-dialdehydecellulose (DAC) membranes as a potential tissue-engineering scaffold has been developed from methylolcellulose by the simultaneous water-induced phase separation and sodium chloride salt leaching techniques, followed by oxidation with sodium periodate in water. Membrane pores increased in size with increasing weight or particle size of the sodium chloride salt. The porosity of the membrane was not affected by the salt particle size, but it increased with an increase in the salt weight to 60%. At higher salt weight percentages, no significant change in the membrane porosity was observed. The oxidation step had no effect on the membrane porosity or pore size. All membranes with a porosity value ranging between 87 and 93% showed interconnected porous structures. The use of these membranes as a potential tissue-engineering scaffold was evaluated with the use of human neonatal skin fibroblast cells. Confocal microscopy showed cell attachment and spreading on these membranes. Immunohistochemical tests revealed the presence of collagen type III and fibronectin, indicating that the cells were viable and formed the extracellular matrix. In conclusion, the DAC membrane supports cell adhesion and proliferation and hence shows potential to be used as a tissue-engineering scaffold.

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“…In particular, cultures using either microcarriers [41][42][43][44] or hollow-ber modules 31,45,46 have been described. In 2004, Demetriou et al reported the development of the HepatAssist BAL device 26 .…”

Section: Discussionmentioning

confidence: 99%

Development of a New Bioartificial Liver Support System Using a Radial-flow Bioreactor

Aoki

Murakami

Yasuda³

et al. 2015

Showa Univ J Med Sci

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: There is an increasing number of patients with severe liver disease that requires whole organ transplantation or living-related split liver transplantation. This has resulted in a shortage of donor organs, which is particularly problematic and still awaits resolution. Bioarti cial liver BAL support systems have been developed with the aim of supporting patients with life-threatening liver disease until their liver recovers. Here, we describe a high performance three-dimensional rat hepa tocyte culture system using a radial-ow bioreactor RFB with a polyvinyl alcohol PVA membrane as a small-scale BAL support system. Hepatocytes from male Sprague-Dawley rat livers were isolated and divided into two groups as follows. Group A : isolated hepatocytes were maintained in culture medium as controls ; and group B : isolated hepatocytes were injected into the medium cham ber of the RFB-PVA culture system. Sampling was carried out every 48 h to ana lyze the concentrations of ammonia and albumin in the medium. Light and elec tron microscopic examination of hepatocytes explanted from the PVA membrane was also performed. Albumin production and urea synthesis by cells in group B were both signi cantly higher than in group A. Hematoxylin-Eosin staining of the cells in group B showed that three-dimensional cell masses were attached to the PVA membrane. It also showed that the cells were stably proliferating in the porous spaces of the PVA. Scanning electron microscopic images of group B also showed clusters of hepatocytes attached to the PVA membrane. Hepatocyte clus ters growing in the RFB-PVA culture system retained their biological function and were stable in the porous spaces of the PVA membrane. This cell culture system may be useful for the development of new BAL support systems.

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Multiporous Cellulose Microcarrier for the Development of a Hybrid Artificial Liver Using Isolated Hepatocytes

Cited by 46 publications

References 8 publications

The Future Prospects of Microbial Cellulose in Biomedical Applications

The Future Prospects of Microbial Cellulose in Biomedical Applications

Fabrication and evaluation of porous 2,3‐dialdehydecellulose membrane as a potential biodegradable tissue‐engineering scaffold

Development of a New Bioartificial Liver Support System Using a Radial-flow Bioreactor

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