Multipotent neural cell lines can engraft and participate in development of mouse cerebellum

Snyder, Evan Y.; Deitcher, David L.; Walsh, Christopher A.; Arnold-Aldea, Susan A.; Hartwieg, Erika; Cepko, Constance L.

doi:10.1016/0092-8674(92)90204-p

Cited by 885 publications

(631 citation statements)

References 47 publications

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“…Nevertheless, the boundary between these two lineages may be crossed after different manipulations, including oncogene-induced immortalization (Snyder et al, 1992;Gao and Hatten, 1994) or expansion in vitro (Klein et al, 2005;Lee et al, 2005). Our transplants of freshly dissociated cells are consistent with an exclusive origin of granule cells from the external granular layer, because these neurons were absent when periventricular donors were used.…”

Section: Potentiality Of the Cerebellar Gabaergic Interneuron Progenisupporting

confidence: 55%

Different Types of Cerebellar GABAergic Interneurons Originate from a Common Pool of Multipotent Progenitor Cells

et al. 2006

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Different cerebellar phenotypes are generated according to a precise spatiotemporal schedule, in which projection neurons precede local interneurons. Glutamatergic neurons develop from the rhombic lip, whereas GABAergic neurons originate from the ventricular neuroepithelium. Progenitors in these germinal layers are committed toward specific phenotypes already at early ontogenetic stages. GABAergic interneurons are thought to derive from a subset of ventricular zone cells, which migrate in the white matter and proliferate up to postnatal life. During this period, different interneuron categories are produced according to an inside-out sequence, from the deep nuclei to the molecular layer (we show here that nuclear interneurons are also born during late embryonic and early postnatal days, after glutamatergic and GABAergic projection neurons). To ask whether distinct interneuron phenotypes share common precursors or derive from multiple fate-restricted progenitors, we examined the behavior of embryonic and postnatal rat cerebellar cells heterotopically/ heterochronically transplanted to syngenic hosts. In all conditions, donor cells achieved a high degree of integration in the cerebellar cortex and deep nuclei and acquired GABAergic interneuron phenotypes appropriate for the host age and engraftment site. Therefore, contrary to other cerebellar types, which derive from dedicated precursors, GABAergic interneurons are produced by a common pool of progenitors, which maintain their full developmental potentialities up to late ontogenetic stages and adopt mature identities in response to local instructive cues. In this way, the numbers and types of inhibitory interneurons can be set by spatiotemporally patterned signals to match the functional requirements of developing cerebellar circuits.

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Section: Potentiality Of the Cerebellar Gabaergic Interneuron Progenisupporting

confidence: 55%

Different Types of Cerebellar GABAergic Interneurons Originate from a Common Pool of Multipotent Progenitor Cells

et al. 2006

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“…In the case of genetically manipulated human NSC clones (v-myc+), however, the propagating gene product v-myc is undetectable in donor human cells after engraftment (Flax et al, 1998), despite the fact that the brains of transplant recipients contain numerous stably engrafted donor-derived cells. In addition, previous observations showed the invariant absence of brain tumors derived from implanted v-myc-propagated NSCs, even after several years in rodents (Snyder et al, 1992;Jeong et al, 2003;Chu et al, 2003Chu et al, , 2004a or in primates (Ourednik et al, 2001), which is consistent with the loss of v-myc expression from stably engrafted NSCs following transplantation. We also detected no tumorigenesis in the present study.…”

Section: Discussionsupporting

confidence: 80%

Noninvasive method of immortalized neural stem-like cell transplantation in an experimental model of Huntington's disease

Lee

Park

Lee

et al. 2006

Journal of Neuroscience Methods

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“…At low frequency, some cells di erentiate into sympathoadrenergic progenitor cells. Similarly, the v-Myc-immortalized progenitor cells from neonatal cerebellum are multipotent and can integrate into cerebellum in a nontumorigenic, cytoarchitecturally appropriate manner (Snyder et al, 1992). These cells di erentiate into at least two neuronal types or into glia consistent with their sites of engraftment.…”

Section: Mechanisms Of Transformation and Tumor Progressionmentioning

confidence: 99%

The v-myc oncogene

Lee

Reddy

1999

Oncogene

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Multipotent neural cell lines can engraft and participate in development of mouse cerebellum

Cited by 885 publications

References 47 publications

Different Types of Cerebellar GABAergic Interneurons Originate from a Common Pool of Multipotent Progenitor Cells

Different Types of Cerebellar GABAergic Interneurons Originate from a Common Pool of Multipotent Progenitor Cells

Noninvasive method of immortalized neural stem-like cell transplantation in an experimental model of Huntington's disease

The v-myc oncogene

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