2013
DOI: 10.1155/2013/860457
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Multipyrene Tandem Probes for Point Mutations Detection in DNA

Abstract: Here we report design, synthesis and characterization of highly sensitive, specific and stable in biological systems fluorescent probes for point mutation detection in DNA. The tandems of 3′- and 5′-mono- and bis-pyrene conjugated oligo(2′-O-methylribonucleotides), protected by 3′-“inverted” thymidine, were constructed and their potential as new instruments for genetic diagnostics was studied. Novel probes have been shown to exhibit an ability to form stable duplexes with DNA target due to the stabilizing effe… Show more

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Cited by 5 publications
(4 citation statements)
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“…There should be no specific signal unless both adjacent components of the probe hybridize with the NA target, providing the proper positioning of fluorophores necessary for the excimer or exciplex formation. Previously, the potential of pyrene-labeled dual probes was demonstrated for the detection of RNA in cellular extracts by means of time-resolved fluorescence spectroscopy [ 89 ], and for discrimination of SNPs in NA with the use of excimer- [ 90 , 91 ] or exciplex-generating probes [ 13 , 14 ].…”
Section: Fluorescent Biosensorsmentioning
confidence: 99%
See 2 more Smart Citations
“…There should be no specific signal unless both adjacent components of the probe hybridize with the NA target, providing the proper positioning of fluorophores necessary for the excimer or exciplex formation. Previously, the potential of pyrene-labeled dual probes was demonstrated for the detection of RNA in cellular extracts by means of time-resolved fluorescence spectroscopy [ 89 ], and for discrimination of SNPs in NA with the use of excimer- [ 90 , 91 ] or exciplex-generating probes [ 13 , 14 ].…”
Section: Fluorescent Biosensorsmentioning
confidence: 99%
“…A new detection strategy utilizing highly sensitive and specific fluorescent dual 2′- O -methyl RNA probes for the detection of point mutations in DNA was described in [ 91 , 120 , 121 ]. The detection strategy is based on the use of tandems of 3′- and 5′-mono- and bispyrene-modified 2′- O -methyl RNA probes (structures of modified nucleotides are shown in Figure 11 for 5′Nbpyr , and Figure 15 for 5′Nmpyr , 3′Nmpyr and 3′Nbpyr ) forming an excimer when two or more pyrene groups are brought into close proximity upon hybridization of the components of the probes with DNA.…”
Section: Fluorescent Biosensorsmentioning
confidence: 99%
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“…As one can see, many probes are now chemically modified at the backbone. Growing applications in fluorescence microscopy put requirements on the dyes; therefore, more photostable variants find applications [ 48 , 55 , 74 , 82 , 83 , 84 , 85 , 86 , 87 , 88 ]. Another important aspect is growing requirements for multiplexing in vitro and in vivo, i.e., demand for a broader spectrum of excitation and emission wavelengths provided by FRET pairs [ 74 , 81 ].…”
Section: Förster Resonance Energy Transfer-based Probes In Nucleicmentioning
confidence: 99%