Multiresidue method for the determination of 227 pesticides in hot pepper (Capsicum annuum L.) by liquid chromatography with tandem mass spectrometry†

Abstract

A high-throughput, rapid, and efficient modified QuEChERS (quick, easy, cheap, effective, rugged, and safe) method with a simple cleanup procedure has been developed for simultaneously determining 227 pesticides in pepper samples by liquid chromatography with tandem mass spectrometry (running time: 10 min). Pesticide residues were extracted/partitioned with an acetonitrile/DisQuE QuEChERS pouch, and the resulting samples were cleaned up with different methods: dispersive solid-phase extraction with primary secondary amines or multiwalled carbon nanotubes and graphitized carbon solid mini cartridge column. The results indicated that multiwalled carbon nanotubes dispersive sorbents achieved the best recoveries and had less matrix interference. The numbers of pesticides with a recovery in the range of 70-120% were 199 at a spiked level of 40 μg/kg. The correlation coefficients (r(2)) for 227 pesticides were above 0.99, while the limits of quantitation of pesticides in pepper samples ranged from 0.13 to 13.51 μg/kg (S/N = 10), and the limits of detection ranged from 0.04 to 4.05 μg/kg (S/N = 3). The relative standard deviations of approximately 197 pesticides were below 20% at spiked levels of 40 μg/kg. Based on these results, the proposed method was chosen as the most suitable cleanup procedure for the determination of multiresidue pesticides in pepper samples.

Determination of metrafenone in bitter gourd and soil by GC with ECD

“…Few studies have recently reported analytical methods for measuring metrafenone. Metrafenone residue analysis is mainly performed via liquid chromatography with tandem mass spectrometry (LC-MS) (Yoann, David, Alain, & Pierre, 2010;Zhu, Zhao, Feng, & Kim, 2014). The above methods were all multi-residue analytical method of 227 and 242 kinds of pesticides, the probably range of recovery was from 70% to 120% in hot pepper, not detailed.…”

A reliable screening of mycotoxins and pesticide residues in paprika using ultra-high performance liquid chromatography coupled to high resolution Orbitrap mass spectrometry

“…The spices of paprika are controlled for highly-persistent nonpolar pesticide residues, monitored by gas chromatography (GC) coupled to QqQ-MS/MS or time-of-flight (TOF-MS) detectors (Fernandes et al, 2014), and the various polar pesticides are assessed by UHPLC coupled to QqQ-MS/MS (Masi a, Blasco, & Pic o, 2014;Zhu, Zhao, Nan Feng, & Han Kim, 2014) and OrbitrapeHRMS (Albert, Kramer, Scheeren, & Engelhard, 2014) …”

The perfluoroalkyl carboxylic acids (PFCAs) and perfluoroalkane sulfonates (PFSAs) contamination level in spices

“…Although spices contribute little to weight on our dish, they can be important contributors to our daily antioxidants [7] intake, especially in dietary cultures where spices are used regularly [8]. Due to the origin, cultivation conditions and type of processing, spices may also be a source of some contaminants such as pesticides [9,10], heavy metals [11], illegal dyes [12,13], mycotoxins [14,15] and perfluoroalkyl substances.…”