Multiscale pink-beam microCT imaging at the ESRF-ID17 biomedical beamline

Mittone, Alberto; Fardin, Luca; Lillo, Francesca Di; Fratini, Michela; Requardt, H.; Mauro, Anthony; Homs-Regojo, Roberto Arturo; Douissard, Paul-Antoine; Barbone, Giacomo E.; Stroebel, Johannes; Romano, Mariele; Massimi, Lorenzo; Provinciali, Ginevra Begani; Palermo, Francesca; Bayat, Sam; Cedola, Alessia; Coan, Paola; Bravin, Alberto

doi:10.1107/s160057752000911x

Cited by 24 publications

(19 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sub-micron experiments were conducted at both the ID17 beamline (ESRF) and the TOMCAT beamline (SLS). The experiment at ID17 was performed with a filtered polychromatic beam using a peak energy of 55 keV and a sample-to-detector distance of 1.2 m. As a detector system, a PCO.edge 5.5 camera with a 10 × Optique Peter (Lentilly, France) was used, achieving an effective pixel size of 0.7 × 0.7 µm 2 [ 26 ]. CT acquisitions consisted of 50 ms per projection.…”

Section: Methodsmentioning

confidence: 99%

Multiscale X-ray phase contrast imaging of human cartilage for investigating osteoarthritis formation

et al. 2021

Self Cite

View full text Add to dashboard Cite

Background The evolution of cartilage degeneration is still not fully understood, partly due to its thinness, low radio-opacity and therefore lack of adequately resolving imaging techniques. X-ray phase-contrast imaging (X-PCI) offers increased sensitivity with respect to standard radiography and CT allowing an enhanced visibility of adjoining, low density structures with an almost histological image resolution. This study examined the feasibility of X-PCI for high-resolution (sub-) micrometer analysis of different stages in tissue degeneration of human cartilage samples and compare it to histology and transmission electron microscopy. Methods Ten 10%-formalin preserved healthy and moderately degenerated osteochondral samples, post-mortem extracted from human knee joints, were examined using four different X-PCI tomographic set-ups using synchrotron radiation the European Synchrotron Radiation Facility (France) and the Swiss Light Source (Switzerland). Volumetric datasets were acquired with voxel sizes between 0.7 × 0.7 × 0.7 and 0.1 × 0.1 × 0.1 µm3. Data were reconstructed by a filtered back-projection algorithm, post-processed by ImageJ, the WEKA machine learning pixel classification tool and VGStudio max. For correlation, osteochondral samples were processed for histology and transmission electron microscopy. Results X-PCI provides a three-dimensional visualization of healthy and moderately degenerated cartilage samples down to a (sub-)cellular level with good correlation to histologic and transmission electron microscopy images. X-PCI is able to resolve the three layers and the architectural organization of cartilage including changes in chondrocyte cell morphology, chondrocyte subgroup distribution and (re-)organization as well as its subtle matrix structures. Conclusions X-PCI captures comprehensive cartilage tissue transformation in its environment and might serve as a tissue-preserving, staining-free and volumetric virtual histology tool for examining and chronicling cartilage behavior in basic research/laboratory experiments of cartilage disease evolution.

show abstract

Section: Methodsmentioning

confidence: 99%

Multiscale X-ray phase contrast imaging of human cartilage for investigating osteoarthritis formation

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…The irradiation sessions were performed at the MRT dedicated hutch of ID17, with the animal stage placed on a remotely controlled goniometer located at about 38.5 m from the radiation source (further details in [ 31 ]). Animals were irradiated under anesthesia with isoflurane inhalation (4% for 2 minutes) for induction followed by an intraperitoneal xylazine/ketamine injection (64.5/5.4 mg/kg).…”

Section: Methodsmentioning

confidence: 99%

“…3.25 3 µm 3 Voxel Size XPCI-CT at ID17, ESRF These scans were realized in the imaging hutch of the ID17 beamline, placed around 150 m downstream the X-ray source. The sample was illuminated by a laminar, monochromatic 35 keV X-ray beam and placed 1.8 m from the imaging detector, which is a sCMOS PCO.Edge 5.5 [39], equipped with a 50 µm-thick LuAG:Ce scintillator screen and a 2× magnification indirect conversion optics [31]. For each CT scan, 4000 projections were acquired over 360° with an exposure time per projection of 30 ms. Every rat brain was entirely imaged requiring 5-6 vertical CT scans.…”

Section: Xpci-ct Imagingmentioning

confidence: 99%

A Multi-Scale and Multi-Technique Approach for the Characterization of the Effects of Spatially Fractionated X-ray Radiation Therapies in a Preclinical Model

Romano

Bravin

Mittone

et al. 2021

Cancers

Self Cite

View full text Add to dashboard Cite

The purpose of this study is to use a multi-technique approach to detect the effects of spatially fractionated X-ray Microbeam (MRT) and Minibeam Radiation Therapy (MB) and to compare them to seamless Broad Beam (BB) irradiation. Healthy- and Glioblastoma (GBM)-bearing male Fischer rats were irradiated in-vivo on the right brain hemisphere with MRT, MB and BB delivering three different doses for each irradiation geometry. Brains were analyzed post mortem by multi-scale X-ray Phase Contrast Imaging–Computed Tomography (XPCI-CT), histology, immunohistochemistry, X-ray Fluorescence (XRF), Small- and Wide-Angle X-ray Scattering (SAXS/WAXS). XPCI-CT discriminates with high sensitivity the effects of MRT, MB and BB irradiations on both healthy and GBM-bearing brains producing a first-time 3D visualization and morphological analysis of the radio-induced lesions, MRT and MB induced tissue ablations, the presence of hyperdense deposits within specific areas of the brain and tumor evolution or regression with respect to the evaluation made few days post-irradiation with an in-vivo magnetic resonance imaging session. Histology, immunohistochemistry, SAXS/WAXS and XRF allowed identification and classification of these deposits as hydroxyapatite crystals with the coexistence of Ca, P and Fe mineralization, and the multi-technique approach enabled the realization, for the first time, of the map of the differential radiosensitivity of the different brain areas treated with MRT and MB. 3D XPCI-CT datasets enabled also the quantification of tumor volumes and Ca/Fe deposits and their full-organ visualization. The multi-scale and multi-technique approach enabled a detailed visualization and classification in 3D of the radio-induced effects on brain tissues bringing new essential information towards the clinical implementation of the MRT and MB radiation therapy techniques.

show abstract

“…A more relaxed resolution with enhanced photon flux would be more suitable. For example, filtered white beams are utilized to generate intense high-energy X-rays above 30 keV from a bending-magnet or wiggler source (Di Michiel et al, 2005;Hoshino et al, 2017;Stevenson et al, 2017;Mittone et al, 2020). At SPring-8 beamline BL28B2, X-rays from a bending-magnet source were filtered using a 0.5 mm-thick tungsten plate and a 2 mm-thick lead plate to achieve a flux density of approximately 10 11 photons s À1 mm À2 with a peak energy of 200 keV and an energy width of 100 keV (ÁE/E = 0.5) (Hoshino et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Double-multilayer monochromators for high-energy and large-field X-ray imaging applications with intense pink beams at SPring-8 BL20B2

Koyama¹,

Senba²,

Yamazaki³

et al. 2022

J Synchrotron Rad

View full text Add to dashboard Cite

In this study, double-multilayer monochromators that generate intense, high-energy, pink X-ray beams are designed, installed and evaluated at the SPring-8 medium-length (215 m) bending-magnet beamline BL20B2 for imaging applications. Two pairs of W/B4C multilayer mirrors are designed to utilize photon energies of 110 keV and 40 keV with bandwidths of 0.8% and 4.8%, respectively, which are more than 100 times larger when compared with the Si double-crystal monochromator (DCM) with a bandwidth of less than 0.01%. At an experimental hutch located 210 m away from the source, a large and uniform beam of size 14 mm (V) × 300 mm (H) [21 mm (V) × 300 mm (H)] was generated with a high flux density of 1.6 × 109 photons s−1 mm−2 (6.9 × 1010 photons s−1 mm−2) at 110 keV (40 keV), which marked a 300 (190) times increase in the photon flux when compared with a DCM with Si 511 (111) diffraction. The intense pink beams facilitate advanced X-ray imaging for large-sized objects such as fossils, rocks, organs and electronic devices with high speed and high spatial resolution.

show abstract

Multiscale pink-beam microCT imaging at the ESRF-ID17 biomedical beamline

Cited by 24 publications

References 45 publications

Multiscale X-ray phase contrast imaging of human cartilage for investigating osteoarthritis formation

Multiscale X-ray phase contrast imaging of human cartilage for investigating osteoarthritis formation

A Multi-Scale and Multi-Technique Approach for the Characterization of the Effects of Spatially Fractionated X-ray Radiation Therapies in a Preclinical Model

Double-multilayer monochromators for high-energy and large-field X-ray imaging applications with intense pink beams at SPring-8 BL20B2

Contact Info

Product

Resources

About