Multispectral optoacoustic tomography for non-invasive disease phenotyping in pediatric spinal muscular atrophy patients

Regensburger, Adrian P.; Wagner, Alexandra L.; Danko, Vera; Jüngert, Jörg; Federle, Anna; Klett, Daniel; Schuessler, Stephanie; Buehler, Adrian; Roos, Andreas; Lochmüller, Hanns; Woelfle, Joachim; Trollmann, Regina; Waldner, Maximilian J.; Knieling, Ferdinand

doi:10.1016/j.pacs.2021.100315

Cited by 24 publications

(37 citation statements)

References 52 publications

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“…To limit this potential shortcoming, all ROIs were reviewed by two readers. Furthermore, anatomical relocation cannot be performed as precisely as during muscle imaging [10] , [35] , [36] , as physiological alterations, especially gas and stool filling, require slight modifications of probe positioning. Furthermore, the inferior mesenteric artery was not assessed.…”

Section: Discussionmentioning

confidence: 99%

Multispectral optoacoustic tomography of the human intestine – temporal precision and the influence of postprandial gastrointestinal blood flow

et al. 2023

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Section: Discussionmentioning

confidence: 99%

Multispectral optoacoustic tomography of the human intestine – temporal precision and the influence of postprandial gastrointestinal blood flow

et al. 2023

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“…In a translational setting before transplantation, Hysi et al demonstrated that OAI can be used to determine kidney organ quality [ 13 ]. As shown in chronic inflammatory bowel diseases [ 46 , 47 ], rheumatoid arthritis [ 48 ], and muscular diseases [ 49 – 51 ], this could also be achieved via transcutaneous in vivo imaging. In the future, due to the limited penetration depth, it may also be possible to implement this technique in a catheter-based approach to allow contrast-free visualization of single renal vessels [ 52 – 54 ].…”

Section: Discussionmentioning

confidence: 99%

High-resolution label-free mapping of murine kidney vasculature by raster-scanning optoacoustic mesoscopy: an ex vivo study

et al. 2022

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Background Chronic kidney disease (CKD) is a global burden affecting both children and adults. Novel imaging modalities hold great promise to visualize and quantify structural, functional, and molecular organ damage. The aim of the study was to visualize and quantify murine renal vasculature using label-free raster scanning optoacoustic mesoscopy (RSOM) in explanted organs from mice with renal injury. Material and methods For the experiments, freshly bisected kidneys of alpha 8 integrin knock-out (KO) and wildtype mice (WT) were used. A total of n=7 female (n=4 KO, n=3 WT) and n=6 male animals (n=2 KO, n=4 WT) aged 6 weeks were examined with RSOM optoacoustic imaging systems (RSOM Explorer P50 at SWL 532nm and/or ms-P50 imaging system at 532 nm, 555 nm, 579 nm, and 606 nm). Images were reconstructed using a dedicated software, analyzed for size and vascular area and compared to standard histologic sections. Results RSOM enabled mapping of murine kidney size and vascular area, revealing differences between kidney sizes of male (m) and female (f) mice (merged frequencies (MF) f vs. m: 52.42±6.24 mm2 vs. 69.18±15.96 mm2, p=0.0156) and absolute vascular area (MF f vs. m: 35.67±4.22 mm2 vs. 49.07±13.48 mm2, p=0.0036). Without respect to sex, the absolute kidney area was found to be smaller in knock-out (KO) than in wildtype (WT) mice (WT vs. KO: MF: p=0.0255) and showed a similar trend for the relative vessel area (WT vs. KO: MF p=0.0031). Also the absolute vessel areas of KO compared to WT were found significantly different (MF p=0.0089). A significant decrease in absolute vessel area was found in KO compared to WT male mice (MF WT vs. KO: 54.37±9.35 mm2 vs. 34.93±13.82 mm2, p=0.0232). In addition, multispectral RSOM allowed visualization of oxygenated and deoxygenated parenchymal regions by spectral unmixing. Conclusion This study demonstrates the capability of RSOM for label-free visualization of differences in vascular morphology in ex vivo murine renal tissue at high resolution. Due to its scalability optoacoustic imaging provides an emerging modality with potential for further preclinical and clinical imaging applications.

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“…Multispectral optoacoustic tomography (MSOT) uses multiwavelength laser tissues and has implemented reflected‐ultrasound computed tomography (RUCT) for anatomical guidance. The signal intensities of this hybrid technology were used in dedicated diseases to reflect the disease state in inflammatory bowel diseases 8,9 and neuromuscular disorders (NMDs) 10,11 . In 5q‐related spinal muscular atrophy (SMA), a life‐limiting genetic NMD, where the loss of the survival motor neuron protein leads to muscular atrophy and, if untreated, early death, 12,13 OAI revealed novel insights into muscular transformation 11 .…”

Section: Introductionmentioning

confidence: 99%

Hybrid reflected‐ultrasound computed tomography versus B‐mode‐ultrasound for muscle scoring in spinal muscular atrophy

Danko

Jüngert

Schuessler

et al. 2023

Journal of Neuroimaging

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Background and Purpose: Novel light-and sound-based technologies like multispectral optoacoustic tomography (MSOT) with co-registered reflected-ultrasound computed tomography (RUCT) could add additional value to conventional ultrasound (US) for disease phenotyping in pediatric spinal muscular atrophy (SMA). The aim of this study was to investigate the quality of RUCT compared to US for qualitative and quantitative assessment of imaging neuromuscular disorders.Methods: Subanalyzing the MSOT SMA study, 288 RUCT and 276 US images from 10 SMA patients (mean age 9.0 ± 3.7) and 10 gender-and age-matched healthy volunteers (HV; mean age 8.7 ± 4.3) were analyzed for quantitative (grayscale levels [GSLs]) and qualitative (echogenicity, distribution pattern, Heckmatt scale, and muscle texture) muscle changes. RUCT and US measures were further correlated with clinical standard motor outcomes.

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Multispectral optoacoustic tomography for non-invasive disease phenotyping in pediatric spinal muscular atrophy patients

Cited by 24 publications

References 52 publications

Multispectral optoacoustic tomography of the human intestine – temporal precision and the influence of postprandial gastrointestinal blood flow

Multispectral optoacoustic tomography of the human intestine – temporal precision and the influence of postprandial gastrointestinal blood flow

High-resolution label-free mapping of murine kidney vasculature by raster-scanning optoacoustic mesoscopy: an ex vivo study

Hybrid reflected‐ultrasound computed tomography versus B‐mode‐ultrasound for muscle scoring in spinal muscular atrophy

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