Multistep involvement of glutathione with salicylic acid and ethylene to combat environmental stress

Ghanta, Srijani; Datta, R. K.; Bhattacharyya, Dipto; Sinha, Ragini; Kumar, Deepak; Hazra, Saptarshi; Mazumdar, Aparupa Bose; Chattopadhyay, Sharmila

doi:10.1016/j.jplph.2014.03.002

Cited by 58 publications

(56 citation statements)

References 54 publications

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“…This observation, together with our previous report (Ghanta et al, 2014), suggests an induction of ET biosynthesis by GSH. ET biosynthesis is a two-step process where the first step is catalyzed by ACC synthase (ACS) and the final step is catalyzed by ACO.…”

Section: Raising Transgenic Arabidopsis With Enhanced Gsh Content (Atsupporting

confidence: 90%

“…Later on, it has been noted that GSH may also act through NPR1-independent pathway to increase intracellular hydrogen peroxide, which activates SA signaling (Han et al, 2013). Only very recently, we have reported that an enhanced GSH level can provide resistance against B. cinerea infection, presumably through its crosstalk with ET (Ghanta et al, 2014). However, the mechanism of this GSH-ET interplay is still unexplored.…”

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confidence: 98%

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Glutathione regulates ACC synthase transcription via WRKY33 and ACC oxidase by modulating mRNA stability to induce ethylene synthesis during stress

et al. 2015

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Glutathione (GSH) plays a fundamental role in plant defense-signaling network. Recently, we have established the involvement of GSH with ethylene (ET) to combat environmental stress. However, the mechanism of GSH-ET interplay still remains unexplored. Here, we demonstrate that GSH induces ET biosynthesis by modulating the transcriptional and posttranscriptional regulations of its key enzymes, 1-aminocyclopropane-1-carboxylate synthase (ACS) and 1-aminocyclopropane-1-carboxylate oxidase (ACO). Transgenic Arabidopsis (Arabidopsis thaliana) plants with enhanced GSH content (AtECS) exhibited remarkable up-regulation of ACS2, ACS6, and ACO1 at transcript as well as protein levels, while they were down-regulated in the GSH-depleted phytoalexin deficient2-1 (pad2-1) mutant. We further observed that GSH induced ACS2 and ACS6 transcription in a WRKY33-dependent manner, while ACO1 transcription remained unaffected. On the other hand, the messenger RNA stability for ACO1 was found to be increased by GSH, which explains our above observations. In addition, we also identified the ACO1 protein to be a subject for S-glutathionylation, which is consistent with our in silico data. However, S-glutathionylation of ACS2 and ACS6 proteins was not detected. Further, the AtECS plants exhibited resistance to necrotrophic infection and salt stress, while the pad2-1 mutant was sensitive. Exogenously applied GSH could improve stress tolerance in wild-type plants but not in the ET-signaling mutant ethylene insensitive2-1, indicating that GSH-mediated resistance to these stresses occurs via an ET-mediated pathway. Together, our investigation reveals a dual-level regulation of ET biosynthesis by GSH during stress.

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Section: Raising Transgenic Arabidopsis With Enhanced Gsh Content (Atsupporting

confidence: 90%

mentioning

confidence: 98%

Glutathione regulates ACC synthase transcription via WRKY33 and ACC oxidase by modulating mRNA stability to induce ethylene synthesis during stress

et al. 2015

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“…Out of stable chloroplast-targeted γ-ECS overexpressed transgenic tobacco lines, NtGp11 demonstrated better potential in GSH content; stress-responsive transcripts/proteins accumulation as well as disease-resistance capacity 14 and hence was further evaluated here to assess the abiotic stress tolerance prospective.…”

Section: Discussionmentioning

confidence: 99%

“…14 had the homology with ACC oxidase which is an important enzyme of ethylene biosynthetic pathway and it can be induced by stress. 14,35 Spot no. 39 had identified to heme oxygenase I which earlier reported to play protective role in plants against oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

“…[10][11][12][13] In our recent investigation it was also noted that GSH may act through multistep signaling pathways to mitigate environmental stresses. 14 The protective role of GSH against low temperature stress was also demonstrated by chemical or genetic manipulation of its level. 15 Besides an increase in the size of the glutathione pool, a high GSH/GSSG ratio is also necessary for the efficient removal of the reactive oxygen species generated under stress conditions.…”

Section: Introductionmentioning

confidence: 99%

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Proteomic profiling of γ-ECSoverexpressed transgenicNicotianain response to drought stress

Kumar

Datta²,

Sinha

et al. 2014

Plant Signaling & Behavior

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The contribution of Glutathione (Gsh) in drought stress tolerance is an established fact. however, the proteins which are directly or indirectly related to the increased level of Gsh in response to drought stress are yet to be known. To explore this, here, transgenic tobacco plants (NtGp11) overexpressing gamma-glutamylcysteine synthetase (γ-ECS) was tested for tolerance against drought stress. NtGp11 conferred tolerance to drought stress by increased germination rate, water retention, water recovery, chlorophyll, and proline content compared with wild-type plants. semi-quantitative RT-PcR analysis revealed that the transcript levels of stress-responsive genes were higher in NtGp11 compared with wild-type in response to drought stress. Two-dimensional gel electrophoresis (2-De) coupled with MaLDI TOF-TOF Ms/Ms analysis has been used to identify 43 differentially expressed proteins in response to drought in wild-type and NtGp11 plants. The results demonstrated the up-accumulation of 58.1% of proteins among which 36%, 24%, and 20% of them were related to stress and defense, carbon metabolism and energy metabolism categories, respectively. Taken together, our results demonstrated that Gsh plays an important role in combating drought stress in plants by inducing stress related genes and proteins like hsP70, chalcone synthase, glutathione peroxidase, thioredoxin peroxidase, acc oxidase, and heme oxygenase I.

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Phytohormonal Roles in Plant Responses to Heavy Metal Stress: Implications for Using Macrophytes in Phytoremediation of Aquatic Ecosystems

Nguyen

Sesin

Kisiała

et al. 2020

Enviro Toxic and Chemistry

114

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Heavy metals can represent a threat to the health of aquatic ecosystems. Unlike organic chemicals, heavy metals cannot be eliminated by natural processes such as their degradation into less toxic compounds, and this creates unique challenges for their remediation from soil, water, and air. Phytoremediation, defined as the use of plants for the removal of environmental contaminants, has many benefits compared to other pollution-reducing methods. Phytoremediation is simple, efficient, cost-effective, and environmentally friendly because it can be carried out at the polluted site, which simplifies logistics and minimizes exposure to humans and wildlife. Macrophytes represent a unique tool to remediate diverse environmental media since they can accumulate heavy metals from contaminated sediment via roots, from water via submerged leaves, and from air via emergent shoots. In this review, a synopsis is presented about how plants, especially macrophytes, respond to heavy metal stress and we propose potential roles that phytohormones can play in the alleviation of metal toxicity in the aquatic environment. We focus on the uptake, translocation, and accumulation mechanisms of heavy metals in organs of macrophytes and give examples of how phytohormones interact with plant defense systems under heavy metal exposure. We advocate for a more in-depth understanding of these processes to inform more effective metal remediation techniques from metal-polluted waterbodies.

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Multistep involvement of glutathione with salicylic acid and ethylene to combat environmental stress

Cited by 58 publications

References 54 publications

Glutathione regulates ACC synthase transcription via WRKY33 and ACC oxidase by modulating mRNA stability to induce ethylene synthesis during stress

Glutathione regulates ACC synthase transcription via WRKY33 and ACC oxidase by modulating mRNA stability to induce ethylene synthesis during stress

Proteomic profiling of γ-ECSoverexpressed transgenicNicotianain response to drought stress

Phytohormonal Roles in Plant Responses to Heavy Metal Stress: Implications for Using Macrophytes in Phytoremediation of Aquatic Ecosystems

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