Multivalent nanobody-based sandwich enzyme-linked immunosorbent assay for sensitive detection of porcine reproductive and respiratory syndrome virus

Sun, Mingxia; Sun, Yue; Yang, Yongbo; Zhao, Man; Cao, Dan; Zhang, Minmin; Xia, Dasong; Wang, Tao; Gao, Yanfei; Wang, Shanghui; Wang, Haiwei; Cai, Xuehui; An, Tongqing

doi:10.1016/j.ijbiomac.2023.128896

Search citation statements

Order By: Relevance

Paper Sections

Select...

Application Of the N Protein In Clinical Detection1

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2024

2025

Publication Types

Select...

Article2

Book1

Relationship

Self Cite0

Independent3

Authors

Journals

Cited by 3 publications

(1 citation statement)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This method offers several advantages, such as a stable expression system and ease of operation. Sun et al ( 2023 ) selected two high-affinity nanobodies to develop sandwich ELISA. The modified technique showed increased sensitivity.…”

Section: Application Of the N Protein In Clinical Detectionmentioning

confidence: 99%

Research progress on the N protein of porcine reproductive and respiratory syndrome virus

Zheng,

Li,

Luo

et al. 2024

Front. Microbiol.

View full text Add to dashboard Cite

Porcine reproductive and respiratory syndrome (PRRS) is a highly contagious disease caused by the porcine reproductive and respiratory syndrome virus (PRRSV). PRRSV exhibits genetic diversity and complexity in terms of immune responses, posing challenges for eradication. The nucleocapsid (N) protein of PRRSV, an alkaline phosphoprotein, is important for various biological functions. This review summarizes the structural characteristics, genetic evolution, impact on PRRSV replication and virulence, interactions between viral and host proteins, modulation of host immunity, detection techniques targeting the N protein, and progress in vaccine development. The discussion provides a theoretical foundation for understanding the pathogenic mechanisms underlying PRRSV virulence, developing diagnostic techniques, and designing effective vaccines.

show abstract

Section: Application Of the N Protein In Clinical Detectionmentioning

confidence: 99%