Multivariate Analysis of the Stability of Spray-Dried<i>Eupenicillium javanicum</i>Peptidases

Neto, Youssef Ali Abou Hamin; Freitas, Luís Alexandre Pedro de; Cabral, Hamilton

doi:10.1080/07373937.2013.853079

Cited by 13 publications

(2 citation statements)

References 20 publications

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“…Spray drying is a cost-effective drying method widely used to stabilize enzymes (Schutyser et al 2012;Assadpour;Jafari 2019;Bajaj et al 2021). However, when subjected to the high temperatures used in C B A the spray drying process, some enzymes may be denatured and, consequently, lose their catalytic activity (Hamin et al 2014). A variety of excipients are often used as bulking agents and protective additives to stabilize the enzymes (Ohtake et al 2011;Emami et al 2018).…”

Section: Discussionmentioning

confidence: 99%

Spray drying of cellulases produced by Bacillus sp. SMIA-2

Cruz,

Talma,

Barbosa

et al. 2024

Biosci. J.

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Microbial cellulases present biotechnological potential to be used in several industries, including food, brewery and wine, industrial waste for chemical feedstock, animal feed, pulp and paper, agriculture, textile and detergent production. In this work, cellulases produced by the thermophilic Bacillus sp. SMIA-2 in submerged cultures containing sugarcane bagasse, corn steep liquor and passion fruit rind flour were spray-dried, in an attempt to improve their stability for industrial purposes. The cellulases were spray dried and analyzed before and after the drying process and subsequent storage. A Central Composite Design (CCD) 23 was used to investigate the effect of different concentrations of arabic gum and microcrystalline cellulose, as well as the spray dryer inlet temperature on the cellulase spray drying process. The results evidenced that the combination of 1.0 % (w/v) arabic gum and 1.0% (w/v) microcrystalline cellulose, at inlet temperature of 70 °C, was effective in maintaining the activities of both avicelases (avicel-hydrolyzing enzymes) and carboxymethylcellulases (carboxymethylcellulose-hydrolyzing enzymes - CMCase). The dried avicelase was completely preserved when stored at 5oC, while the CMCase retained 89% of its activity, which indicates promising potential for industrial uses, especially in detergent formulations.

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Section: Discussionmentioning

confidence: 99%

Spray drying of cellulases produced by Bacillus sp. SMIA-2

Cruz,

Talma,

Barbosa

et al. 2024

Biosci. J.

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“…Among these microbial sources, the filamentous fungus Eupenicillium javanicum has been shown to produce different products with industrial interests, such as antifungal compounds (Nakadate et al, 2007, 2008), endoglucanase, β-glucosidase, pectinase, xylanase (Tao et al, 2011), and a protease stable to spray dryer process (Hamin Neto et al, 2014). …”

Section: Introductionmentioning

confidence: 99%

Analysis of the Specificity and Biochemical Characterization of Metalloproteases Isolated from Eupenicillium javanicum Using Fluorescence Resonance Energy Transfer Peptides

Neto

Oliveira

et al. 2017

Front. Microbiol.

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Enzymes have important features that may facilitate their application in industrial processes and have been used as alternatives to chemical catalysts. In particular, proteases can be isolated from microorganisms, which provide important sources of advantageous enzymes for industrial processes. For example, Eupenicillium javanicum is a filamentous fungus that has been shown to express industrially applicable enzymes and chemical components, such as antifungal compounds. The biotechnological potential of E. javanicum and proteases made us search a novel protease from this microorganism. The macromolecule was isolated, the main biochemical properties was evaluated, and the specificity of the protease subsites was determined. The protease was produced under solid-state bioprocess with wheat bran and isolated by two chromatography steps with yield of 27.5% and 12.4-fold purification. The molecular mass was estimated at 30 kDa. The N-terminal sequence of the first 20 amino acid residues was AVGAGYNASVALALEKALNN. The enzyme presented higher proteolytic activity at pH 6.0 and 60°C. The protease is stable at wide range of pH values and temperatures and in the presence of surfactants. The “primed” side of the catalytic site showed the highest catalytic efficiency of the enzyme isolated from E. javanicum. The S′1 subsite is responsible for catalyzing the protease reaction with substrates with tyrosine in P′1. These findings provide important insights into the biochemical characterization of a highly active protease from E. javanicum and may facilitate the development of industrial processes involving this protease.

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Spray‐Dried Wheat Gluten Protein Hydrolysate Microcapsules: Physicochemical Properties, Retention of Antioxidant Capability, and Release Behavior Under Simulated Gastrointestinal Digestion Conditions

GanjiVtan,

Hosseini Ghaboos,

Sadeghi Mahoonak

et al. 2024

Food Science & Nutrition

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Wheat gluten is a by‐product of the wheat starch industry, rich in bioactive peptides. Spray drying is an effective method for improving the stability of bioactive compounds. So, the aim of this study was to produce gluten hydrolysate by different proteases (alcalase, pancreatin, and trypsin) at different times (40–200 min). The hydrolysate with the strongest antioxidant potential (produced by pancreatin after 200 min of hydrolysis) was encapsulated by spray drying. The effect of wall material's type (maltodextrin, potato starch, and their combination at different ratios) on the encapsulation efficiency, physicochemical properties (moisture content, solubility, water activity, tapped and bulk density, and hygroscopicity), release behavior under simulated gastrointestinal digestion conditions, and morphology of microcapsules were evaluated. The microcapsules produced by maltodextrin and potato starch at a 30:70 ratio possessed the highest water activity (0.36), encapsulation efficiency (85.79%), and moisture content (8.2%). An increase in maltodextrin concentration increased the solubility, bulk, and tapped density. SEM images showed that microparticles were spherical with wrinkled surfaces. The microcapsules showed higher stability than free gluten hydrolysate. The combination of maltodextrin and potato starch at a 30:70 ratio could control the release of gluten hydrolysate under simulated gastrointestinal conditions. As a result, the use of maltodextrin and potato starch carriers at a 30:70 ratio in spray drying could effectively protect the bioactive properties of gluten hydrolysate and control its release.

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Multivariate Analysis of the Stability of Spray-DriedEupenicillium javanicumPeptidases

Cited by 13 publications

References 20 publications

Spray drying of cellulases produced by Bacillus sp. SMIA-2

Spray drying of cellulases produced by Bacillus sp. SMIA-2

Analysis of the Specificity and Biochemical Characterization of Metalloproteases Isolated from Eupenicillium javanicum Using Fluorescence Resonance Energy Transfer Peptides

Spray‐Dried Wheat Gluten Protein Hydrolysate Microcapsules: Physicochemical Properties, Retention of Antioxidant Capability, and Release Behavior Under Simulated Gastrointestinal Digestion Conditions

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