Multivariate optimisation and validation of a capillary electrophoresis method for the analysis of resveratrol in a nutraceutical

Orlandini, Serena; Giannini, Iacopo; Pinzauti, S.; Furlanetto, Sandra

doi:10.1016/j.talanta.2007.06.024

Cited by 31 publications

(15 citation statements)

References 27 publications

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“…Taking into account the physicochemical properties of the target compounds, high-performance liquid chromatography (HPLC) is the technique most commonly used, employing different detection modes like ultraviolet or diode array (Cvejic et al 2010;Zhou et al 2007;Du et al 2007;Enguo et al 2007;Abert Vian et al 2005), chemiluminescence (Zhou et al 2004), electrochemistry (Zhang et al 2007; Kahoun et al 2008), fluorimetry (Grippi et al 2008;Rodríguez-Delgado et al 2001), or mass spectrometry (Sun et al 2006;Hamm et al 2010;Jaitz et al 2010;Nicoletti et al 2007;Counet et al 2006;Montsko et al 2008). As an alternative to HPLC, gas chromatography-mass spectrometry (GC-MS) (Soleas et al 2001;Luan et al 2000) and capillary electrophoresis (Spanilá et al 2005;Zhang et al 2009;Orlandini et al 2008) have also been employed.…”

Section: Introductionmentioning

confidence: 99%

Liquid Chromatographic Determination of Resveratrol and Piceid Isomers in Honey

et al. 2011

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In this work, a liquid chromatography method with diode array and fluorescence detection and electrospray ionization mass spectrometry (LC-DAD-FLD and LC-ESI-MS) has been developed for the determination of residues of resveratrol in all its forms (free isomers and glycosylates) in honey. This procedure involves a solidphase extraction on polymeric cartridges for the isolation of trans/cis-isomers of resveratrol and piceid from diluted honey samples. Chromatographic separation of the analytes was performed in isocratic mode on a C 18 column (150 mm×4.6 mm i.d., 5 μm) operated at 30°C. The mobile phase consisted of a mixture of water with 1% formic acid and acetonitrile (75/25, v/v), and the flow rate was set at 1.0 mL/min. Average analyte recoveries were from 71% to 113% in replica sets of fortified honey samples. The detection limits when using LC-DAD-FLD were between 40 and 120 μg/kg, meanwhile for LC-ESI-MS were between 1.4 and 14.6 μg/kg. The proposed method was applied to the analysis of honey samples collected from treated beehives in experimental apiaries after a preliminary field trial, and they were not found any residues over the detection limits of the studied compounds.

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Section: Introductionmentioning

confidence: 99%

Liquid Chromatographic Determination of Resveratrol and Piceid Isomers in Honey

et al. 2011

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“…Ultrasonic extraction was the most frequently used method due to its efficiency and easy manipulation. Indeed, it was employed in about two‐thirds (180 out of 271 articles) of cited references (Supporting Information Tables 1–7) 4–271. Other conventional extraction methods included reflux 6, 14, 15, 21, 45, 74, 84, 87, 97, 117, 121, 123, 131, 134, 157, 162, 164, 169, 171, 172, 176, 179, 182–184, 205, 212, 227, 235, 236, 259, Soxhlet extraction 23, 89, 91, 128, 131, 244, heating extraction 22, 39, 51...…”

Section: Sample Preparationmentioning

confidence: 99%

“…Therefore, triangle and tetrahedron optimization methods based on the interaction of different factors were adapted for the selection of BGE in CE fingerprint development 182, 183. Chemometrics‐based techniques such as factorial design 4, 48, 199, 237, 249, 262 and response surface methodology 160, 217 were also employed. Factorial design of 3 2 and 2 3 was used for the optimization of CE analysis of phenolic acids in exotic fruits 48 and butenolides in Piper malacophyllum 249, respectively.…”

Section: Separationmentioning

confidence: 99%

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CE and CEC analysis of phytochemicals in herbal medicines

et al. 2011

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CE and CEC, due to their versatility and high efficiency, have attracted great interest in the analysis of phytochemicals in herbs and their preparations. Previously, we reviewed the analysis of phytochemical bioactive compounds by CE in 2006 (Electrophoresis 2006, 27, 4808-4819) or CEC in 2010 (Electrophoresis 2010, 31, 260-277). This review followed the previous studies and covered the literature published since 2006 for CE and 2009 for CEC (excluding those mentioned in the two previous reviews), which emphasized the development of CE and CEC techniques in phytochemical analysis. In addition, sample preparation and detection were also discussed.

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“…where t 2 and t 1 are the migration times and w 2 and w 1 are the corresponding peak widths at the bases of adjacent peak pairs [24]. For overlapping and co-eluting peaks, the resolution was estimated using data from Snyder [25] A single response objective function (Chromatographic Exponential Function; CEF, Equation 2) was used to evaluate the quality of each electropherogram with regard to resolution and time [26].…”

Section: Screening Designmentioning

confidence: 99%

A capillary electrophoresis method for the determination of selected biogenic amines and amino acids in mammalian decomposition fluid

Swann

Forbes

Lewis

2010

Talanta

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A simple capillary zone electrophoresis method for the determination of selected biogenic amines (tyramine and tryptamine) and amino acids (tryptophan, phenylalanine and tyrosine) in mammalian decomposition fluids is presented. Separations were carried out in a fused silica capillary (75microm i.d., total length 65cm, effective length 56cm) with detection by ultraviolet absorbance spectrophotometry at 200nm. In order to improve resolution and total analysis time, the method was subjected to optimisation utilising a chemometric approach. A screening design was carried out followed by a central composite design (CCD), using peak resolution and total analysis time as response factors. The influences of four experimental variables (pH, background electrolyte concentration, percentage of organic modifier (methanol) and applied voltage) were investigated. Optimum separation conditions were determined to be; a background electrolyte of boric acid (70mM) adjusted to pH 9.5 with 0.1M sodium hydroxide with 32% methanol (v/v). Applied voltage was 30kV, with the resulting current being less than 26microA. Under these conditions the analytes were separated within 12min. Tryptamine, tyramine, tryptophan, tyrosine and phenylalanine were identified by migration time and spiking in porcine decomposition fluids.

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Multivariate optimisation and validation of a capillary electrophoresis method for the analysis of resveratrol in a nutraceutical

Cited by 31 publications

References 27 publications

Liquid Chromatographic Determination of Resveratrol and Piceid Isomers in Honey

Liquid Chromatographic Determination of Resveratrol and Piceid Isomers in Honey

CE and CEC analysis of phytochemicals in herbal medicines

A capillary electrophoresis method for the determination of selected biogenic amines and amino acids in mammalian decomposition fluid

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