Multivariate quantitative analysis of quality trend based on non-volatile characteristic components in different Panax notoginseng samples using HPLC

Li, Chao; Yunhua, Qin; Yang, Qianxu; You, Jinmao; Liu, Zhihua; Han, Jun; Li, E’xian; Zhang, Chengming

doi:10.1016/j.jpba.2020.113127

Cited by 12 publications

(4 citation statements)

References 3 publications

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“…Principal component analysis (PCA) is a multivariate statistical method [ 16 ]. While preserving the original variable information as much as possible, the basic idea is to transform the original high-dimensional data into a low-dimensional feature variable of linearly independent through an orthogonal transformation.…”

Section: Methodsmentioning

confidence: 99%

Research on anthracnose grade of Camellia oleifera based on the combined LIBS and THz technology

Li¹,

Qiu²,

Zhan³

et al. 2022

Plant Methods

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Background Anthracnose of Camellia oleifera is a very destructive disease that commonly occurs in the Camellia oleifera industry, which severely restricts the development of the Camellia oleifera industry. In the early stage of the Camellia oleifera suffering from anthracnose, only the diseased parts of the tree need to be repaired in time. With the aggravation of the disease, the diseased branches need to be eradicated, and severely diseased plants should be cut down in time. At present, aiming at the problems of complex experiments and low accuracy in detecting the degree of anthracnose of Camellia oleifera, a method is proposed to detect the degree of anthracnose of Camellia oleifera leaves by using terahertz spectroscopy (THz) combined with laser-induced breakdown spectroscopy (LIBS), so as to realize the rapid, efficient, non-destructive and high-precision determination of the degree of anthracnose of Camellia oleifera. Results Mn, Ca, Ca II, Fe and other elements in the LIBS spectrum of healthy and infected Camellia oleifera leaves with different degrees of anthracnose are significantly different, and the Terahertz absorption spectra of healthy Camellia oleifera leaves, and Camellia oleifera leaves with different degrees of anthracnose there are also significant differences. Partial least squares discriminant analysis (PLS-DA), support vector machine (SVM), and linear discriminant analysis (LDA) are used to establish the fusion spectrum anthracnose classification model of Camellia oleifera. Among them, the Root mean square error of prediction (RMSEP) and the prediction determination coefficient R2p of THz-LIBS-CARS-PLS-DA of prediction set are 0.110 and 0.995 respectively, and the misjudgment rate is 1.03%; The accuracy of the modeling set of THz (CARS)-LIBS (CARS)-SVM is 100%, and the accuracy of prediction set is 100%, after preprocessing of the multivariate scattering correction (MSC), the accuracy of the THz-LIBS-MSC-CARS modeling set is 100%, and the accuracy of prediction set is 100%; The accuracy rate of THz-LIBS-MSC-CARS-LDA of modeling set is 98.98%, and the accuracy rate of the prediction set is 96.87%. Conclusion The experimental results show that: the SVM model has higher qualitative analysis accuracy and is more stable than the PLS-DA and LDA models. The results showed that: the THz spectrum combined with the LIBS spectrum could be used to separate healthy Camellia oleifera leaves from various grades of anthracnose Camellia oleifera leaves non-destructively, quickly and accurately.

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Section: Methodsmentioning

confidence: 99%

Research on anthracnose grade of Camellia oleifera based on the combined LIBS and THz technology

Li¹,

Qiu²,

Zhan³

et al. 2022

Plant Methods

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“…Panax notoginseng is a perennial herb belonging to the genus Panax anomaly Araliaceae that is widely used in traditional Chinese medicine. P. notoginseng contains a variety of chemical components including saponins, flavonoids, sterols, polysaccharides, amino acids, fatty acids, and various trace elements ( Li et al, 2020 ). Specifically, the root is beneficial to human health due to its anti-atherosclerotic, antioxidant, anti-inflammatory, anti-hyperlipidemic, hypoglycemic, neuroprotective, anti-coagulation, and anti-tumor properties ( Wang et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Solid-State Fermentation With Aspergillus cristatus Enhances the Protopanaxadiol- and Protopanaxatriol-Associated Skin Anti-aging Activity of Panax notoginseng

Lee

Reddy

Ryu³

et al. 2021

Front. Microbiol.

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A metabolomics approach was used to profile metabolites of Panax notoginseng fermented with Aspergillus cristatus in two ways, liquid-state fermentation (LF-P) and solid-state fermentation (SSF-P) and examine metabolite markers representing antioxidant activity and skin anti-aging. Protopanaxadiol (PPD) and protopanaxatriol (PPT) contents were higher in SSF-P than in LF-P and showed a multiplicative increase over the fermentation period of four days. PPD and PPT levels also correlated with antioxidant and anti-aging effects in skin, based on the mRNA expression of dermal extracellular matrix components. In the bioactivity validation assays, PPD and PPT significantly improved the expression of type-I collagen, fibrillin-1, and elastin in human dermal fibroblasts from both young and old subjects; these were comparable with the effects of the SSF-P extracts. Overall, our results suggest that changes in the metabolites of P. notoginseng fermented with A. cristatus enhance the quality and availability of bioactive compounds associated with skin anti-aging.

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“…In order to increase the weight, nonmedicinal parts with less active ingredients are often added to the raw materials, such as the addition of stem and leaf powder in pure root powder of Panax notoginseng. 6 These adulterants often contained the same characteristic ingredients as authentic herbal medicines, making it difficult to discriminate them with conventional methods. In addition, its market value was mainly determined by the cultivation years and geographical origins.…”

Section: Introductionmentioning

confidence: 99%

Multisource information fusion strategies of mass spectrometry and Fourier transform infrared spectroscopy data for authenticating the age and parts of Vietnamese ginseng

Liu

Zuo

et al. 2021

Journal of Chemometrics

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Aiming at two different classification tasks in the field of quality evaluation of valuable Chinese herbal medicine, the applicability of data fusion strategy based on different complementary analysis techniques was studied. In this study, attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) and ultra-performance liquid chromatography quadrupole timeof-flight mass spectrometry (UPLC-QTOF/MS) were used to analyze the different parts (including roots, stems, leaves, and fibrils) of 2-to 5-year-old Vietnamese ginseng. The multivariate classification models (orthogonal partial least squares discrimination analysis, OPLS-DA; support vector machine, SVM) were established using low-level and mid-level data fusion methods to identify different parts and age of Vietnamese ginseng. The OPLS-DA model have shown that the fusion data matrix with low-level fusion processing could separate Vietnamese ginseng samples with different growth years to the greatest extent, whereas the single data matrix had partial overlap. But 100% of prediction set classification accuracy could be achieved. The SVM model combined with two parameter optimization algorithms (grid search, GS; genetic algorithm, GA) was used to identify Vietnamese ginseng samples with different ages. The mid-level fusion strategy based on the recursive feature elimination (RFE) features variable extraction method was more suitable for SVM model. In the model established by combining the two parameter optimization methods, the identification effect can reach 83.33%. The results showed that data fusion, as an effective strategy, could distinguish different ages and parts of Vietnamese ginseng.

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Multivariate quantitative analysis of quality trend based on non-volatile characteristic components in different Panax notoginseng samples using HPLC

Cited by 12 publications

References 3 publications

Research on anthracnose grade of Camellia oleifera based on the combined LIBS and THz technology

Research on anthracnose grade of Camellia oleifera based on the combined LIBS and THz technology

Solid-State Fermentation With Aspergillus cristatus Enhances the Protopanaxadiol- and Protopanaxatriol-Associated Skin Anti-aging Activity of Panax notoginseng

Multisource information fusion strategies of mass spectrometry and Fourier transform infrared spectroscopy data for authenticating the age and parts of Vietnamese ginseng

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