Multivariate statistical analysis of tropane alkaloids in Anisodus tanguticus (Maxim.) Pascher from different regions to trace geographical origins

Abstract

Anisodus tanguticus (Maxim.) Pascher is an important Tibetan folk medicine and the source of tropane alkaloids (TAs) grown in Qinghai-Tibet Plateau. There are marked differences in quality of A. tanguticus from geographic areas. The aim of present research was to establish a method for the quantitative analysis of TAs coupled with chemometrics analysis to trace geographical origins. Qualitative analysis of TAs in A. tanguticus was carried out using ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry and quantitative analysis of TAs in different plant organs from different geographical origin was achieved. Contents of TAs were subjected to the principal component analysis, and orthogonal partial least-squares discriminant analysis. The contents of the three marker compounds (anisodamine, anisodine and atropine) in the roots and acrial parts of A. tanguticus were positive correlated and varied significantly from different geographical origins. Principal component analysis, and orthogonal partial least-squares discriminant analysis results showed excellent discrimination between different geographical origin of A. tanguticus. This study could provide comprehensive evaluation and further utilization of A. tanguticus resources.

Multivariate Statistical Analysis of Metabolites in Anisodus tanguticus (Maxim.) Pascher to Determine Geographical Origins and Network Pharmacology

“…A total of 29 compounds were detected and qualified. Five tropane alkaloids (anisodine, convolamine, anisodamine, atropine, noratropine), which have tropane ring characteristics, were identified in our previous study (Chen et al, 2021 ). Except for five tropane alkaloids, physochlain was identified based its tropane ring characteristics.…”

Predicting potential and quality distribution of Anisodus tanguticus (Maxim.) Pascher under different climatic conditions in the Qinghai–Tibet plateau

“…A. tanguticus has garnered growing interest from pharmaceutical companies due to its role as a source of tropane alkaloids like anisodine, anisodamine, and atropine. These alkaloids find applications in analgesia, anesthesia, antispasmodics, anti-motion sickness, and the treatment of Parkinson’s disease ( Qiu et al., 2021 ; Zhang et al., 2022 ; Chen et al., 2022b , c ). Wild digging and climate change will exacerbate the loss of the endangered plant habitat.…”

A core root bacteria contribute to plant growth and anisodine accumulation of Anisodus tanguticus

“…Pascher, a perennial herb that belongs to the family Solanaceae, is a traditional herbal medicine used by Tibetan medicine. It is found primarily in the alpine and subalpine belts of the Qinghai-Tibetan Plateau at elevations ranging from 2 200 to 4 200 m. Its roots are rich in anisodamine, atropine, scopolamine and anisodine, making it an essential resource plant for tropane alkaloids extraction [ 1 ]. It has the effects of promoting blood circulation and removing blood stasis, analgesic and antispasmodic, and hemostasis, and is commonly used in the treatment of traumatic fractures and bleeding, malignant sores, as well as pain caused by canker, chronic or acute gastroenteritis and biliary ascariasis [ 2 , 3 ].…”