Multiwell photocatalytic microreactor device integrating drug biotransformation modeling and sample preparation on a MALDI target

Gorbunov, Alexander Yu.; Bardin, Alexander; Ilyushonok, Semyon; Kovach, Yakov; Petrenko, A.A.; Суходолов, Н. Г.; Краснов, К. А.; Krasnov, N. V.; Зорин, И. М.; Obornev, Alexander; Babakov, Vladimir; Radilov, A. S.; Podolskaya, Е. P.

doi:10.1016/j.microc.2022.107362

Cited by 8 publications

(3 citation statements)

References 58 publications

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“…дополнительные материалы, табл. S1) и проведена модификация глобина человека по методике, представленной в работе [8]. Объектом исследования стал пептид LLGNVLVC 112 VLAHHFGK, образующийся в результате триптического гидролиза β-субъединицы глобина человека.…”

Section: поступило в редакцию 22 июля 2022 г в окончательной редакции...unclassified

Формирование Наноразмерных Мультимолекулярных Структур Стеарата Лантана С Использованием Монослоев Ленгмюра Для Масс-Спектрометрии С Лазерной Десорбцией/Ионизацией

Горбунов¹,

Подольская²

2022

Письма В Журнал Технической Физики

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Исследована матрично-активированная лазерная десорбция/ионизация (МАЛДИ) с поверхности наноразмерных мультимолекулярных структур на основе монослоев стеарата лантана, наличие которых на поверхности подтверждалось экспериментально по лазерной десорбции лантансодержащих органических ионов. Показано, что такая функционализация поверхности стандартной МАЛДИ-мишени приводит к значительному увеличению выхода ионов целевых пептидов, причем оптимум достигается при толщине пленки порядка шести монослоев. Предложен подход в формате "Лаборатория на мишени", позволяющий проводить специфичную экстракцию пептидов, модифицированных хлорсодержащими соединениями, и включающий следующие стадии: функционализация поверхности мишени, металл-аффинная экстракция, нанесение матрицы, МАЛДИ масс-спектрометрический анализ. Ключевые слова: масс-спектрометрия, матрично-активированная лазерная десорбция/ионизация, поверхность, монослои Ленгмюра, металл-аффинная хроматография.

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Section: поступило в редакцию 22 июля 2022 г в окончательной редакции...unclassified

Формирование Наноразмерных Мультимолекулярных Структур Стеарата Лантана С Использованием Монослоев Ленгмюра Для Масс-Спектрометрии С Лазерной Десорбцией/Ионизацией

Горбунов¹,

Подольская²

2022

Письма В Журнал Технической Физики

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“…The use of this protein as a drug is still an extremely common procedure in improving the quality of life for patients with diabetes. The questions of searching for chromatographic methods for specific isolation and determination are still important. − Thin-film materials containing lanthanide atoms, as an example of nanotechnology, are actively used in many fields. − Also, materials containing lanthanide atoms are being actively studied as materials for bioorganic sensors, − including for determining the activity of insulin and analytical applications in the analysis of insulin content in complex systems. ,− Taking into account all of the problems associated with the production and application of such materials for proteomics, the purpose of this work is to demonstrate the possibility of using films containing lanthanide ions for insulin adsorption.…”

Section: Introductionmentioning

confidence: 99%

Thin Films of Lanthanide Stearates as Modifiers of the Q-Sense Device Sensor for Studying Insulin Adsorption

et al. 2022

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This article presents new possibilities of using thin films of lanthanide stearates as sorbent materials. Modification of the Q-sense device resonator with monolayers of lanthanide stearates by the Langmuir–Schaeffer method made it possible to study the process of insulin protein adsorption on the surface of new thin-film sorbents. The resulting films were also characterized by compression isotherms, chemical analysis, scanning electron microscopy, and mass spectrometry. The transition of stearic acid to salt was recorded by IR spectroscopy. Using the LDI MS method, the main component of thin films, lanthanide distearate, was established. The presence of Eu 2+ in thin films was revealed. In the case of europium stearate, the maximum value of insulin adsorption was obtained, −1.67·10 −10 mole/cm 2 . The findings suggest the possibility of using thin films of lanthanide stearates as a sorption material for the proteomics determination of the quantitative protein content in complex fluid systems by specific adsorption on modified surfaces and isolation of such proteins from complex mixtures.

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“…Accordingly, it seemed interesting to evaluate the specific and selective properties of the obtained FLa multimolecular structures using the example of human globin adducts with chlorine-containing compounds. We obtained amodiaquine oxidation products (see additional materials, Table S1) and modified human globin according to the method presented in the paper [8]. The object of study was the peptide LLGNVLVC 112 VLAHHFGK, which is formed as a result of tryptic hydrolysis of the β-subunit of human globin.…”

mentioning

confidence: 99%

Fabrication of nanoscale multimolecular structures of lanthanum stearate using Langmuir monolayers for laser desorption/ionization mass spectrometry

Yu.

Podolskaya

2022

Technical Physics Letters

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Matrix-assisted laser desorption/ionization (MALDI) from the surface of nanosized multimolecular structures based on lanthanum stearate monolayers (FLa) has been studied. The presence of FLa on the surface of MALDI target was confirmed experimentally by laser desorption of lanthanum-containing organic ions. MALDI target functionalization with FLa is shown to significantly increase the yield of target peptide ions, with the optimum being achieved at a film thickness of about 6 monolayers. An approach is proposed in a "lab-on-a-plate" format, which allows specific extraction of peptides modified with chlorine-containing compounds and includes the following steps: functionalization of the target surface, metal affinity extraction, matrix deposition and MALDI mass spectrometric analysis. Keywords: mass spectrometry, matrix assisted laser desorption/ionization (MALDI), surface, Langmuir monolayers, metal affinity chromatography.

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Multiwell photocatalytic microreactor device integrating drug biotransformation modeling and sample preparation on a MALDI target

Cited by 8 publications

References 58 publications

Thin Films of Lanthanide Stearates as Modifiers of the Q-Sense Device Sensor for Studying Insulin Adsorption

Fabrication of nanoscale multimolecular structures of lanthanum stearate using Langmuir monolayers for laser desorption/ionization mass spectrometry

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