Murine in vitro cellular models to better understand adipogenesis and its potential applications

Vohra, Muhammad Sufyan; Ahmad, Bilal; Serpell, Christopher J.; Parhar, Ishwar S.; Wong, Eng Hwa

doi:10.1016/j.diff.2020.08.003

Cited by 6 publications

(2 citation statements)

References 235 publications

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“…Interestingly, this study detected STX2 and STX3 in 3T3-L1 adipocytes ( 96 ); however, the requirement for STX2 or STX3 in the processes of insulin-stimulated GLUT4 translocation was not tested. Given the reported issues with 3T3-L1 adipocytes lacking characteristics of primary adipocytes ( 97 ), it will be imperative that inducible adipose-specific STX4 KO mice be evaluated to determine the requirement for STX4 in bona fide adipose tissue in adult mice. Moreover, STX2 or STX3 in skeletal muscle have not been reported.…”

Section: The Physiologic and Mechanistic Roles Of Snares In Glut4 Ves...mentioning

confidence: 99%

Exocytosis Proteins: Typical and Atypical Mechanisms of Action in Skeletal Muscle

Hwang

Thurmond

2022

Front. Endocrinol.

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Insulin-stimulated glucose uptake in skeletal muscle is of fundamental importance to prevent postprandial hyperglycemia, and long-term deficits in insulin-stimulated glucose uptake underlie insulin resistance and type 2 diabetes. Skeletal muscle is responsible for ~80% of the peripheral glucose uptake from circulation via the insulin-responsive glucose transporter GLUT4. GLUT4 is mainly sequestered in intracellular GLUT4 storage vesicles in the basal state. In response to insulin, the GLUT4 storage vesicles rapidly translocate to the plasma membrane, where they undergo vesicle docking, priming, and fusion via the high-affinity interactions among the soluble N-ethylmaleimide sensitive factor attachment protein receptor (SNARE) exocytosis proteins and their regulators. Numerous studies have elucidated that GLUT4 translocation is defective in insulin resistance and type 2 diabetes. Emerging evidence also links defects in several SNAREs and SNARE regulatory proteins to insulin resistance and type 2 diabetes in rodents and humans. Therefore, we highlight the latest research on the role of SNAREs and their regulatory proteins in insulin-stimulated GLUT4 translocation in skeletal muscle. Subsequently, we discuss the novel emerging role of SNARE proteins as interaction partners in pathways not typically thought to involve SNAREs and how these atypical functions reveal novel therapeutic targets for combating peripheral insulin resistance and diabetes.

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Section: The Physiologic and Mechanistic Roles Of Snares In Glut4 Ves...mentioning

confidence: 99%

Exocytosis Proteins: Typical and Atypical Mechanisms of Action in Skeletal Muscle

Hwang

Thurmond

2022

Front. Endocrinol.

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“…In in vitro studies, the 3T3-L1 cell line was used to investigate the underlying mechanism by which FYGL alleviates obesity. 3T3-L1 cells are preadipocytes and normally differentiate into mature adipocytes [ 35 ]. The effects of FYGL on preadipocyte differentiation and mature adipocyte lipid metabolism were investigated in terms of gene and protein expressions in preadipocytes as well as signalling pathways of lipid metabolism in mature adipocytes.…”

Section: Introductionmentioning

confidence: 99%

Balancing adipocyte production and lipid metabolism to treat obesity-induced diabetes with a novel proteoglycan from Ganoderma lucidum

Wang

Zheng

et al. 2023

Lipids Health Dis

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Obesity is often accompanied by metabolic disorder and insulin resistance, resulting in type 2 diabetes. Based on previous findings, FYGL, a natural hyperbranched proteoglycan extracted from the G. lucidum fruiting body, can decrease blood glucose and reduce body weight in diabetic mice. In this article, the underlying mechanism of FYGL in ameliorating obesity-induced diabetes was further investigated both in vivo and in vitro. FYGL upregulated expression of metabolic genes related to fatty acid biosynthesis, fatty acid β-oxidation and thermogenesis; downregulated the expression of insulin resistance-related genes; and significantly increased the number of beige adipocytes in db/db mice. In addition, FYGL inhibited preadipocyte differentiation of 3T3-L1 cells by increasing the expression of FABP-4. FYGL not only promoted fatty acid synthesis but also more significantly promoted triglyceride degradation and metabolism by activating the AMPK signalling pathway, therefore preventing fat accumulation, balancing adipocyte production and lipid metabolism, and regulating metabolic disorders and unhealthy obesity. FYGL could be used as a promising pharmacological agent for the treatment of metabolic disorder-related obesity.

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Engineering and Characterization of a Biomimetic Microchip for Differentiating Mouse Adipocytes in a 3D Microenvironment

et al. 2022

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Murine in vitro cellular models to better understand adipogenesis and its potential applications

Abstract: The version in the Kent Academic Repository may differ from the final published version. Users are advised to check http://kar.kent.ac.uk for the status of the paper. Users should always cite the published version of record.

Cited by 6 publications

References 235 publications

Exocytosis Proteins: Typical and Atypical Mechanisms of Action in Skeletal Muscle

Exocytosis Proteins: Typical and Atypical Mechanisms of Action in Skeletal Muscle

Balancing adipocyte production and lipid metabolism to treat obesity-induced diabetes with a novel proteoglycan from Ganoderma lucidum

Engineering and Characterization of a Biomimetic Microchip for Differentiating Mouse Adipocytes in a 3D Microenvironment

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