Murine Insulinoma Cell-Conditioned Medium with ΒETA2/Neurod1 Transduction Efficiently Induces the Differentiation of Adipose-Derived Mesenchymal Stem Cells into β-Like Cells both In Vitro and In Vivo

Kikuchi, Kôichi; Yabe, Shigeharu; Konno, Masamitsu; Ishii, Hideshi; Nishida, Naohiro; Koseki, Junichi; Fukuda, Satsuki; Tomimaru, Yoshito; Hama, Naoki; Wada, Hiroshi; Kobayashi, Shogo; Eguchi, Hidetoshi; Tanemura, Masahiro; Ito, Toshinori; Lee, Eun Young; Mukai, Eri; Miki, Takashi; Doki, Yuichiro; Mori, Masaki; Hamazaki, Tatsuo S.; Nagano, Hiroaki; Okochi, Hitoshi

doi:10.4172/2157-7633.1000221

Cited by 1 publication

(3 citation statements)

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“…Culture media were replaced every 2 days. The conditioned medium was generated according to the previous study by Kawamoto et al (12). In detail, these hepatocytes grew to ≤50% confluence with hepatocyte culture medium.…”

Section: Methodsmentioning

confidence: 99%

“…ADSCs can be obtained in high yield with minimal discomfort under local anesthesia (10,11). Therefore, ADSCs are considered a useful source for regenerative therapy (12). However, in particular endodermal types of cells, the differentiation method has not been well established and differentiation efficiency is extremely low.…”

Section: Introductionmentioning

confidence: 99%

“…Thus, the development of an efficient differentiation method is extremely important. Several studies suggested that conditioned medium contains several undefined factors (8,12). These undefined factors induce the stem cells to certain specific types of cells (1).…”

Section: Introductionmentioning

confidence: 99%

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Fetal hepatocyte-derived culture medium elicits adipocyte differentiation to bile duct cell lineages in a mouse model

et al. 2018

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Abstract. Fetal cells in the developmental stages function with a distinct mechanism in comparison to adult tissues, which may be a useful source for regenerative medicine in postnatal medicine; however, the precise molecular mechanism remains to be elucidated fully. The present study investigated murine fetal hepatocytes, which were cultured in vitro, and the supernatants were used for the culture with murine adipose tissue-derived cells. Notably, the results indicated that fetal hepatocyte-derived culture medium elicits the induction of differentiation of adipose tissue-derived cells to bile duct cell lineages, but not to hepatocyte lineages in mice. This indicates that fetal cells possess the multi potentials, which are already absent in adults, and may be useful for regenerative medicine in future.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%