Murine mammary stem/progenitor cell isolation: Different method matters?

Gao, Hui; Dong, Qian; Chen, Yuanhong; Zhang, Fuchuang; Wu, Anqi; Shi, Yuanshuo; Bandyopadhyay, Abhik; Daniel, Benjamin J.; Huang, Changjiang; Sun, LuZhe

doi:10.1186/s40064-016-1787-3

Cited by 5 publications

(2 citation statements)

References 12 publications

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“…The dual combination of these surface markers (See Supplementary Fig. 6 ) were shown to have the least tendency of contaminating gated basal cells with non-epithelial cells, especially in the C57BL/6J background 96 , which is the strain of mice used in our study. Flow cytometry was performed using the BD Accuri TM C6 and analyzed with FlowJo (version 10).…”

Section: Methodsmentioning

confidence: 96%

BAD regulates mammary gland morphogenesis by 4E-BP1-mediated control of localized translation in mouse and human models

et al. 2021

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Elucidation of non-canonical protein functions can identify novel tissue homeostasis pathways. Herein, we describe a role for the Bcl-2 family member BAD in postnatal mammary gland morphogenesis. In Bad3SA knock-in mice, where BAD cannot undergo phosphorylation at 3 key serine residues, pubertal gland development is delayed due to aberrant tubulogenesis of the ductal epithelium. Proteomic and RPPA analyses identify that BAD regulates focal adhesions and the mRNA translation repressor, 4E-BP1. These results suggest that BAD modulates localized translation that drives focal adhesion maturation and cell motility. Consistent with this, cells within Bad3SA organoids contain unstable protrusions with decreased compartmentalized mRNA translation and focal adhesions, and exhibit reduced cell migration and tubulogenesis. Critically, protrusion stability is rescued by 4E-BP1 depletion. Together our results confirm an unexpected role of BAD in controlling localized translation and cell migration during mammary gland development.

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Section: Methodsmentioning

confidence: 96%

BAD regulates mammary gland morphogenesis by 4E-BP1-mediated control of localized translation in mouse and human models

et al. 2021

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“…The mammary gland contains many epithelial cells, which are rather sensitive to the tissue digestion procedure. If viable epithelial cells are needed, other means of digestion might be applied [44]. Several specific digestion protocols and solutions exist that warrant viable epithelial cells afterwards.…”

Section: Problem: Low Cell Viability Potential Solutionsmentioning

confidence: 99%

Guidelines for DC preparation and flow cytometry analysis of mouse nonlymphoid tissues

et al. 2022

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This article is part of the Dendritic Cell Guidelines article series, which provides a collection of state‐of‐the‐art protocols for the preparation, phenotype analysis by flow cytometry, generation, fluorescence microscopy and functional characterization of mouse and human dendritic cells (DC) from lymphoid organs and various nonlymphoid tissues.DC are sentinels of the immune system present in almost every mammalian organ. Since they represent a rare cell population, DC need to be extracted from organs with protocols that are specifically developed for each tissue. This article provides detailed protocols for the preparation of single‐cell suspensions from various mouse nonlymphoid tissues, including skin, intestine, lung, kidney, mammary glands, oral mucosa and transplantable tumors. Furthermore, our guidelines include comprehensive protocols for multiplex flow cytometry analysis of DC subsets and feature top tricks for their proper discrimination from other myeloid cells. With this collection, we provide guidelines for in‐depth analysis of DC subsets that will advance our understanding of their respective roles in healthy and diseased tissues.While all protocols were written by experienced scientists who routinely use them in their work, this article was also peer‐reviewed by leading experts and approved by all coauthors, making it an essential resource for basic and clinical DC immunologists.

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Developed analytical expression for current harmonic distortion of the PV system’s inverter in relation to the solar irradiance and temperature

2020

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This paper deals with modeling and simulation of the total harmonic distortion of the current (THD I ) dispatched from the inverter and connected to nonlinear load. The change of THD I was examined in relation to the ambient temperature (T ) and solar irradiance (G). The developed model is being used to extract parameters for a given THD I as a function of temperature and solar radiation. This study outlines the working principle of photovoltaic (PV) panel as well as PV array. Off-grid PV system is modeled by using Matlab/Simulink program, and detailed analytical study has been carried out in this work. The design, modeling and simulation of this study are performed from 50 up to 988 W/m 2 for solar irradiance. Harmonic components have negative effects on the steady-voltage stability of the PV system. Therefore, analytical expression is needed for steady-state stability analysis in order to reduce negative effects. Hence, two analytical expressions of THD I were obtained by two new different methods which are statistical package for the social sciences program and genetic expression programming. Eventually, two different methods have been verified by the Matlab/Simulink program in order to find out THD I and demonstrated the effectiveness of the proposed strategy. As a result of this study, it is observed that input current THDI of nonlinear load is too high at low irradiance. It is suggested that active harmonic filters should be used at low irradiance in order to produce better quality energy and avoid damages in the PV system.

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Murine mammary stem/progenitor cell isolation: Different method matters?

Cited by 5 publications

References 12 publications

BAD regulates mammary gland morphogenesis by 4E-BP1-mediated control of localized translation in mouse and human models

BAD regulates mammary gland morphogenesis by 4E-BP1-mediated control of localized translation in mouse and human models

Guidelines for DC preparation and flow cytometry analysis of mouse nonlymphoid tissues

Developed analytical expression for current harmonic distortion of the PV system’s inverter in relation to the solar irradiance and temperature

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