Murine Model for Non-IgE-Mediated Asthma

Kleij, Hanneke P. M. van der; Kraneveld, Aletta D.; Houwelingen, Anneke H. van; Kool, Mirjam; Weitenberg, Andrys C. D.; Redegeld, Frank A.; Nijkamp, Frans P.

doi:10.1023/b:ifla.0000039557.33267.65

Cited by 20 publications

(14 citation statements)

References 31 publications

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“…At the end of this study, the diagnosis of BA was confirmed in 15% and the allergic character of BA was detected in 6% of CVID patients. The results of both studies are consistent with the notion that IgE is not necessary for the development of BA, which has also been shown in mouse models [39]. We therefore conclude that BA may develop, even in a setting of humoral immunodeficiencies, with disturbed IgE production.…”

Section: Discussionsupporting

confidence: 91%

Bronchial Asthma and Bronchial Hyperresponsiveness and Their Characteristics in Patients with Common Variable Immunodeficiency

Milota

Bloomfield

Paračková

et al. 2018

Int Arch Allergy Immunol

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Background: Common variable immunodeficiency (CVID) is one of the most frequent primary immunodeficiencies and is characterized by disturbed immunoglobulin production and dysregulation of the immune system. Results of previous studies suggest a higher prevalence of bronchial asthma (BA) in CVID patients than in the general population. We initiated this study to evaluate lung functions and identify risk factors for BA and bronchial hyperresponsiveness (BHR) in patients with CVID. Methods: Twenty-three patients with CVID were included in this study. In all of them, spirometry and a metacholine bronchoprovocation test were performed. We also investigated the role of atopy, eosinophilic inflammation, and potential risk factors such as gender, age, or immunoglobulin levels at the time of diagnosis. Results: BHR was confirmed in 12 patients (52%), all of whom had normal FEV₁ and FEV₁/FVC. However, BHR-positive patients had significantly decreased MEF25. BHR-positive patients had also more symptoms related to bronchial obstruction, with 8 of them (35%) being suspected of having BA at the end of the study. A higher prevalence of BHR was found in females, with a relative risk of 2.89. Conclusions: An increased prevalence of BHR and BA was detected in CVID patients compared to the general population. BA may develop despite the disturbed immunoglobulin production, and the majority of patients display nonatopic and noneosinophilic properties. These results suggest a limited role of atopy and eosinophilic inflammation in the pathogenesis of BA in CVID patients.

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Section: Discussionsupporting

confidence: 91%

Bronchial Asthma and Bronchial Hyperresponsiveness and Their Characteristics in Patients with Common Variable Immunodeficiency

Milota

Bloomfield

Paračková

et al. 2018

Int Arch Allergy Immunol

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“…Subsequently, a late asthmatic response with the continuation of airway obstruction and the development of airway inflammation can also be observed. Mast cells play an important role in the early asthmatic response in both atopic and nonatopic human asthma (9,42). Allergen can activate mast cells by cross-linking to high-affinity IgE receptors (Fc⑀RI) (40).…”

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confidence: 99%

Early onset airway obstruction in response to organic dust in the horse

Deaton

Deaton²,

Jose‐Cunilleras³

et al. 2007

Journal of Applied Physiology

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Deaton CM, Deaton L, Jose-Cunilleras E, Vincent TL, Baird AW, Dacre K, Marlin DJ. Early onset airway obstruction in response to organic dust in the horse. J Appl Physiol 102: [1071][1072][1073][1074][1075][1076][1077] 2007. First published December 7, 2006; doi:10.1152/japplphysiol.00264.2006.-Equine recurrent airway obstruction (RAO) has been used as a naturally occurring model of human asthma. However, it is unknown whether there is an early-phase response in RAO. The aim of this study was to determine whether exposure to organic dust induces immediate changes in lung function in RAO-affected horses, which could be mediated by airway mast cells. Six RAO-affected horses in remission and six control horses were challenged with hay-straw dust suspension by nebulization. Total respiratory resistance at 1 Hz, measured by forced oscillation, was increased from 0.62 Ϯ 0.09 cmH 2O ⅐ l Ϫ1 ⅐ s (mean Ϯ SE) to 1.23 Ϯ 0.20 cmH2O ⅐ l Ϫ1 ⅐ s 15 min after nebulization in control horses (P ϭ 0.023) but did not change significantly in the RAO group. Total respiratory reactance at 1 Hz (P ϭ 0.005) was significantly lower in the control horses (Ϫ0.77 Ϯ 0.07 cmH2O ⅐ l Ϫ1 ⅐ s) than in the RAO group (Ϫ0.49 Ϯ 0.04 cmH2O ⅐ l Ϫ1 ⅐ s) 15 min after nebulization. Bronchoalveolar lavage fluid (BALF) histamine concentration was significantly elevated 10 and 20 min postnebulization in control horses but not in RAO horses. Minimum reactance at 1 Hz in the early postnebulization period significantly correlated with both prechallenge BALF mast cell numbers (r ϭ Ϫ0.65, P ϭ 0.02) and peak BALF histamine concentration postnebulization (r ϭ Ϫ0.61, P ϭ 0.04). In conclusion, RAO horses, unlike human asthmatic patients, do not exhibit an early-phase response. However, healthy control horses do demonstrate a mild but significant early (Ͻ20 min) phase response to inhaled organic dust. This response may serve to decrease the subsequent dose of dust inhaled and as such provide a protective mechanism, which may be compromised in RAO horses. equine; lung function; allergen HUMAN ASTHMATIC PATIENTS develop an early-phase bronchoconstriction within minutes of exposure to inhaled allergens.

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“…The most important is that a correct strain of mice has to be used. Adler et al [84], as well as Duguet et al [85], showed that Penh and airway resistance/compliance are comparable and correlated in BALB/c, but not in C57BL/6 mice, thus, the BALB/c mice were used in our study.…”

Section: Discussionmentioning

confidence: 94%

Inhalations with Brine Solution from the ‘Wieliczka’ Salt Mine Diminish Airway Hyperreactivity and Inflammation in a Murine Model of Non-Atopic Asthma

Zając

Russjan

Kostrzon

2020

IJMS

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Inhalations with brine solutions are old but underestimated add-ons to pharmacological treatments of inflammatory lung diseases. Although widely used, not all features underlying their action on the respiratory system have been explored. The aim of the present study was to elucidate the mechanism of the beneficial action of inhalations of brine solution from the ‘Wieliczka’ Salt Mine, a Polish health resort, in a murine model of non-atopic asthma. Asthma was induced in BALB/c mice by skin sensitization with dinitrofluorobenzene followed by an intratracheal challenge of cognate hapten. All animals underwent 12 inhalation sessions with brine solution, pure water or physiological saline. Control mice were not inhaled. We found that brine inhalations reduced, as compared to non-inhaled mice, the typical asthma-related symptoms, like airway hyperreactivity (AHR), the infiltration of pro-inflammatory cells into the bronchial tree, and the inflammation of the airways at the level of pro-inflammatory cytokines IL-1α, IL-1β and IL-6. The level of the anti-inflammatory IL-10 was elevated in brine-inhaled mice. Inhalations with pure water increased AHR, whereas saline had no influence, either on AHR or cytokine concentrations. These observations indicate that inhalations with a brine solution from the ‘Wieliczka’ Salt Mine diminish the asthma-related symptoms, mostly by reducing the inflammatory status and by decreasing AHR.

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Murine Model for Non-IgE-Mediated Asthma

Cited by 20 publications

References 31 publications

Bronchial Asthma and Bronchial Hyperresponsiveness and Their Characteristics in Patients with Common Variable Immunodeficiency

Bronchial Asthma and Bronchial Hyperresponsiveness and Their Characteristics in Patients with Common Variable Immunodeficiency

Early onset airway obstruction in response to organic dust in the horse

Inhalations with Brine Solution from the ‘Wieliczka’ Salt Mine Diminish Airway Hyperreactivity and Inflammation in a Murine Model of Non-Atopic Asthma

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