Murine Models for Development of Anti-Infective Therapeutics

Peter, Angela E.; Pola, Sudhakar; Sandeep, B. V.; Rao, Battu Ganga; Kalpana, V. Lakshmi

doi:10.1007/978-981-15-1695-5_31

Cited by 1 publication

(2 citation statements)

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“…To have a better understanding of fungal ocular infections within an animal body and their interactions with pathogens, studies in vivo in murine models have been used 20,48 . We developed a P roseum keratitis murine model, and our results displayed that fungal infection was established only in immunosuppressed mice and developed fungal structures inside the stroma and corneal endothelium close to the lens.…”

Section: Discussionmentioning

confidence: 95%

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Purpureocillium roseum sp. nov. A new ocular pathogen for humans and mice resistant to antifungals

Calvillo‐Medina

Ponce-Angulo

Raymundo

et al. 2020

Mycoses

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BackgroundInfectious keratitis is the main cause of preventable blindness worldwide, with about 1.5–2.0 million new cases occurring per year. This inflammatory response may be due to infections caused by bacteria, fungi, viruses or parasites. Fungal keratitis is a poorly studied health problem.ObjectivesThis study aimed to identify a new fungal species by molecular methods and to explore the possible efficacy of the three most common antifungals used in human keratitis in Mexico by performing in vitro analysis. The capacity of this pathogen to cause corneal infection in a murine model was also evaluated.MethodsThe fungal strain was isolated from a patient with a corneal ulcer. To identify the fungus, taxonomic and phylogenetic analyses (nrDNA ITS and LSU data set) were performed. An antifungal susceptibility assay for amphotericin B, itraconazole and voriconazole was carried out. The fungal isolate was used to develop a keratitis model in BALB/c mice; entire eyes and ocular tissues were preserved and processed for histopathologic examination.Results and conclusionThis fungal genus has hitherto not been reported with human keratitis in Mexico. We described a new species Purpurecillium roseum isolated from corneal infection. P roseum showed resistance to amphotericin B and itraconazole and was sensitive to voriconazole. In vivo study demonstrated that P roseum had capacity to developed corneal infection and to penetrate deeper corneal tissue. The global change in fungal infections has emphasised the need to develop better diagnostic mycology laboratories and to recognise the group of potential fungal pathogens.

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Section: Discussionmentioning

confidence: 95%

“…Animal models, such as in mice, have regularly been used to learn more about the pathophysiology of mycotic keratitis in humans 16 . Murine models have allowed a better understanding of the pathogenic mechanisms and antifungal resistance of fungi 20 …”

Section: Introductionmentioning

confidence: 99%