Murine Models in the Evaluation of Heparan Sulfate-Based Anticoagulants

Mohammed, Bassem M.; Cheng, Qiufang; Ivanov, Ivan; Gailani, David

doi:10.1007/978-1-0716-1398-6_59

Cited by 3 publications

(4 citation statements)

References 48 publications

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“…Although results are validated for most groups in two models, the inherent limitations in sensitivity in each model may influence the ability to detect differences between treatment arms. Although both murine models have been previously validated with clinically relevant controls (18,31,32), the present results are unproven in humans. The majority of the experiments were performed in male mice to reduce animal usage; however, we did perform a confirmatory subset of experiments in female mice without major differences in the results (Supplemental Digital Content Fig.…”

Section: Discussionmentioning

confidence: 78%

Effect of Irrigation Fluid Composition on Hemostasis in Mouse Bleeding Models

Alsaadi

Hassoune

Haldeman

et al. 2022

Shock

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Introduction: Intraoperative irrigation, usually with normal saline (NS), aids in bleeding identification and management. We investigated the effect of different irrigation fluids, with additives, on hemostasis using two bleeding models. Methods: C57BL/6 J mice were subjected to a tail bleed model or uncontrolled abdominal hemorrhage via liver laceration followed by abdominal cavity irrigation. We compared NS, lactated Ringer's (LR), and PlasmaLyte. We examined NS and LR at different temperatures. Normal saline or LR with calcium (Ca2+) or tranexamic acid (TXA) was studied. Results: Compared with room temperature (RT), increasing the temperature of the irrigation fluid to 37°C and 42°C reduced tail vein bleeding times substantially in both NS and LR (all P < 0.001), with no significant differences between the two fluids. At RT, LR, but not PlasmaLyte, substantially reduced bleeding times in comparison to NS (P < 0.0001). Liver injury blood loss was lower with LR (P < 0.01). Normal saline supplemented with 2.7 mEq/L of Ca2+ decreased bleeding time and blood loss volume (P < 0.001 and P < 0.01, respectively) to similar levels as LR. Normal saline with 150 mg/mL of TXA markedly reduced bleeding time (P < 0.0001), and NS with 62.5 mg/mL TXA decreased blood loss (P < 0.01). Conclusion: Whereas Ca2+- and TXA-supplemented NS reduced bleeding, LR remained superior to all irrigation fluid compositions. As LR contains Ca2+, and Ca2+-supplemented NS mirrored LR in response, Ca2+ presence in the irrigation fluid seems key to improving solution's hemostatic ability. Because warming the fluids normalized the choice of agents, the data also suggest that Ca2+-containing fluids such as LR may be more suitable for hemostasis when used at RT.

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Section: Discussionmentioning

confidence: 78%

Effect of Irrigation Fluid Composition on Hemostasis in Mouse Bleeding Models

Alsaadi

Hassoune

Haldeman

et al. 2022

Shock

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“…The animals were crossed to generate sufficient F9 − / F12 −/− males for testing. In the SVB model, the saphenous vein is exposed and punctured with a needle 14,20,21 . After bleeding stops, clot is gently removed to restart bleeding.…”

Section: Figurementioning

confidence: 99%

“…In the SVB model, the saphenous vein is exposed and punctured with a needle. 14 , 20 , 21 After bleeding stops, clot is gently removed to restart bleeding. The number of clots forming over 30 min and the duration of each bleeding episode are recorded (Figure 1C ).…”

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confidence: 99%

A demonstration of factor XI contributing to hemostasis in the absence of factor XII

Mohammed

Cheng

Gailani

2022

Research and Practice in Thrombosis and Haemostasis

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“…According to the latest data released by the international agency for research on cancer (IARC) ( 1 ), the global cancer burden has risen to 19.3 million new cases and 10 million deaths in 2020. The second leading cause of death in cancer patients is deep venous thromboembolism (DVT) ( 2 ). The incidence of postoperative DVT in gynecological malignant tumors is as high as 19.6–38% ( 3 ).…”

Section: Introductionmentioning

confidence: 99%

The Best Evidence for the Prevention and Management of Lower Extremity Deep Venous Thrombosis After Gynecological Malignant Tumor Surgery: A Systematic Review and Network Meta-Analysis

Geng

et al. 2022

Front. Surg.

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Background:To search and obtain the relevant evidence of prevention and management of lower extremity deep venous thrombosis (DVT) after gynecological malignant tumor operation and to summarize the relevant evidence.MethodsWe searched the JBI evidence summary, up to date, the national comprehensive cancer network of the United States, the guide library of the National Institute of clinical medicine of the United Kingdom, PubMed, the Chinese biomedical literature database, CNKI, Wanfang, and other relevant evidence on the prevention and management of DVT in patients with gynecological malignant tumors. It includes clinical practice guidelines, best practice information book, expert consensus, evidence summary, original research, etc. The retrieval time limit is from database establishment till August 20, 2021. Two researchers independently evaluated the literature quality, combined with professional judgment, and extracted the literature that met the standards.ResultsFinally, 18 literatures were included, including eight guidelines, three evidence summaries, four systematic evaluations, two expert consensuses, and one best practice information volume. A total of 26 pieces of the best evidence on the prevention and management of postoperative venous thrombosis in gynecological malignant tumors were summarized. It includes risk assessment, drug prevention, mechanical prevention, management strategy, and health education.ConclusionThis study summarized the best evidence of risk, prevention, and health management of DVT in postoperative patients with gynecological malignant tumors to provide evidence-based basis for clinical nurses and to improve the nursing level.

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Murine Models in the Evaluation of Heparan Sulfate-Based Anticoagulants

Cited by 3 publications

References 48 publications

Effect of Irrigation Fluid Composition on Hemostasis in Mouse Bleeding Models

Effect of Irrigation Fluid Composition on Hemostasis in Mouse Bleeding Models

A demonstration of factor XI contributing to hemostasis in the absence of factor XII

The Best Evidence for the Prevention and Management of Lower Extremity Deep Venous Thrombosis After Gynecological Malignant Tumor Surgery: A Systematic Review and Network Meta-Analysis

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