Murine’s amygdala microstructure and elevated plus maze activities following R. vomitoria root bark and G. latifolium leaf extracts administration

Nduohosewo, Idongesit S; Ekong, Moses B.

doi:10.1007/s12565-020-00527-1

Cited by 6 publications

(9 citation statements)

References 37 publications

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“…It is reported that reserpine, an active alkaloid of RV affects olfaction as well as causing sedation (Bisong et al, 2010;Ekong et al, 2016a), which may also be a reason for reduced feeding ability of the animals administered RV. The present result corroborates the reports of Eluwa et al (2008), and Ekong et al (2014Ekong et al ( , 2015aEkong et al ( , 2016aEkong et al ( , 2018 who reported similar observations. Histologically, the dorsal hippocampal CA1 of the 200, 300 and 400 mg/kg RV groups showed either atrophic or karyorrhectic cells, which are at variance with Ajao et al (2015) and Okon et al (2020) who reported normal histology of the hippocampus at 150-300 mg/kg RV probably due to treatment duration.…”

Section: Discussionsupporting

confidence: 94%

“…These actions may cause general atrophy/shrinkage and functional disruption of this brain region. RV and its constituents have been indicted in neuronal damage (Ekong et al, 2014(Ekong et al, , 2016b(Ekong et al, , 2017a(Ekong et al, , 2000, which the present study also shows.…”

Section: Discussionsupporting

confidence: 79%

“…NF is a neuronal cytoskeletal protein, an intermediate filament that provides structural support for axons, as well as regulating their diameter (Iwanaga et al, 1989). Decreased NF expression indicates adverse protein rearrangement arising prior to alterations in axonal cytoskeleton (Mages et al, 2018), and RV is reported with adverse neuronal activities (Ekong et al, 2020;Nduohosewo and Ekong, 2020), which may have resulted in decreased expression and population in the present study. The present result is in line with Ekong et al (2016b), who reported that RV cause decreased NF expression in the olfactory bulb.…”

Section: Discussionmentioning

confidence: 56%

“…The roots were washed and the bark was separated from the cambium, air-dried for one week and then pulverized into powder using a kitchen blender. The RV root bark powder was extracted using 80% ethanol and processed as previously described (Ekong et al, 2014(Ekong et al, , 2016a.…”

Section: Preparation Of Rauwolfia Vomitoria Rootsmentioning

confidence: 99%

“…RV effect on the central nervous system may be detrimental, especially with its constituent, reserpine, inhibiting monoamine oxidase, vital for the normal synaptic neurotransmission necessary for the control of motor and cognitive functions (Yu et al, 2011;Mirowska-Guzel, and Balkowiec-Iskra, 2014). Behaviourally, RV administration results in decreased locomotor activities and loss of olfaction among others (Eluwa et al, 2008;Ekong et al, 2014Ekong et al, , 2016aBisong et al, 2019). Microstructural reports include alterations in the cerebellum, cerebral cortex and the olfactory bulb (Ekong et al, 2015a(Ekong et al, , 2015b(Ekong et al, , 2016aEkong and Nwakanma, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Characterization of Hippocampal Histomorphology Following R. vomitoria Root Extract Treatment

Ekong

Ekpene

Nwakanma

et al. 2020

IJMSS

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Rauwolfia vomitoria Afzel. is an antipsychotic plant used by several African communities in the management of psychiatric conditions with good outcomes. Concerns about its dosages on brain activity lead to this investigation of its action on the hippocampal microstructure. Twenty-four adult male Wistar rats of average weight 200 g, were assigned into four groups (n = 6): control; 200, 300 and 400 mg/kg body weight of RV root bark extract, respectively. The administration was once daily, and orally for seven days. Daily observation of the animals was done till on day eight when they were sacrificed after deep anaesthesia. Each brain was processed for histology and immunohistochemical studies. Animals in the 200, 300 and 400 mg/kg RV groups appeared generally dull and drowsy, and barely fed. Their hippocampal histology showed neuronal atrophy and karyorrhexis, with no difference in cell count, although the pyramidal cell numbers decreased in the 300 and 400 mg/kg RV groups. Neuron-specific enolase decreased in the 400 mg/kg RV group, while neurofilament decreased in all test groups. Glial fibrillary acidic protein expression and density increased in the 200 and 300 mg/kg RV groups, but not the 400 mg/kg RV group, all compared with the control group. The given doses of RV root bark extract in adult Wistar rats showed sedative activities with hippocampal histopathological changes, which may not be reversible, thereby leading to the hippocampal functional deficit.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 56%

Section: Preparation Of Rauwolfia Vomitoria Rootsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Characterization of Hippocampal Histomorphology Following R. vomitoria Root Extract Treatment

Ekong

Ekpene

Nwakanma

et al. 2020

IJMSS

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Evaluation of the biological efficiency of Terminalia chebula fruit extract against neurochemical changes induced in brain of diabetic rats: an epigenetic study

El-Shamarka,

Aboulthana,

Omar

et al. 2024

Inflammopharmacol

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Diabetes mellitus (DM) is a chronic and progressive metabolic disorder that can stimulate neuroinflammation and increase oxidative stress in the brain. Therefore, the present study was aimed to assess the efficacy of ethanolic Terminalia chebula extract against the neurochemical and histopathological changes induced in the brains of diabetic rats. The study clarified the reduction in oxidative stress induced in the brains of diabetic rats by the significant (P ≤ 0.05) increase in levels of the antioxidants with decreasing the peroxidation products via ethanolic T. chebula extract at both doses (400 and 600 mg/kg). Moreover, T. chebula extract improved the brain integrity by lowering levels of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), β-amyloid (Aβ) content, monocyte chemoattractant protein-1 (MCP-1) and acetylcholine esterase (ACHE) significantly (P ≤ 0.05) in a dose dependent manner compared to brain of diabetic rats. Severe nuclear pyknosis and degeneration were noticed in neurons of the cerebral cortex, hippocampus and striatum in brains of diabetic rats. The severity of these alterations decreased with T. chebula extract at a dose of 600 mg/kg compared to the other treated groups. The different electrophoretic protein and isoenzyme assays revealed that the lowest similarity index (SI%) values exist in the brains of diabetic rats compared to the control group. The quantity of the most native proteins and isoenzyme types increased significantly (P ≤ 0.05) in the brains of diabetic rats, and these electrophoretic variations were completely diminished by T. chebula extract. The study concluded that T. chebula extract ameliorated the biochemical, histopathological and electrophoretic abnormalities induced in the brains of diabetic rats when administered at a dose of 600 mg/kg.

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Adverse effects associated with use of specific dietary supplements: The US Military Dietary Supplement Use Study

Knapik

Trone

Steelman

et al. 2022

Food and Chemical Toxicology

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Murine’s amygdala microstructure and elevated plus maze activities following R. vomitoria root bark and G. latifolium leaf extracts administration

Cited by 6 publications

References 37 publications

Characterization of Hippocampal Histomorphology Following R. vomitoria Root Extract Treatment

Characterization of Hippocampal Histomorphology Following R. vomitoria Root Extract Treatment

Evaluation of the biological efficiency of Terminalia chebula fruit extract against neurochemical changes induced in brain of diabetic rats: an epigenetic study

Adverse effects associated with use of specific dietary supplements: The US Military Dietary Supplement Use Study

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