Musashi1 expression cells derived from mouse embryonic stem cells can be enriched in side population isolated by fluorescence activated cell sorter

Yu, Tao; Zhao, Lei; Lan, Shaoyang; Fan, Miao-Jing; Gong, Yu; Shi, Liu; Yuan, Yuhong; Huang, Kaihong; Chen, Qi‐Kui

doi:10.1186/1471-2121-12-47

Cited by 2 publications

(3 citation statements)

References 56 publications

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“…The approach employed treatment with specific growth and differentiation factors in the culture medium; one of the characteristics of the induced endoderm was an increase in Msi1 expression [48]. A similar approach in murine embryonic stem cells also corroborated the data linking Msi1 to the SP cells and to endoderm cell fate [49]. In fact, SP from murine embryonic stem cells isolated by fluorescence-activated cell sorting is enriched in Msi1- expressing cells.…”

Section: Musashi1 In the Intestinal Epitheliummentioning

confidence: 97%

“…In fact, SP from murine embryonic stem cells isolated by fluorescence-activated cell sorting is enriched in Msi1- expressing cells. More importantly, markers for neural cells and intestinal epithelial cells were detected in the grafts generated by this cell fraction [49]. Finally, sophisticated studies employing reporter mouse models based upon green fluorescent protein knock-in into the Lrg5 locus [37], coupled with cell sorting and transcriptomic/proteomic analysis, have defined the mouse small-intestinal stem cell signature [44].…”

Section: Musashi1 In the Intestinal Epitheliummentioning

confidence: 99%

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The RNA-Binding Protein Musashi1: A Major Player in Intestinal Epithelium Renewal and Colon Cancer Development

Plateroti

Araújo

Silva

et al. 2012

Curr Colorectal Cancer Rep

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Aberrant gene expression is the cause and the consequence of tumorigenesis. A major component of gene expression is translation regulation; a process whose main players are RNA-binding-proteins (RBPs). More than 800 RBPs have been identified in the human genome and several of them have been shown to control gene networks associated with relevant cancer processes. A more systematic characterization of RBPs starts to reveal that similar to transcription factors, they can function as tumor suppressors or oncogenes. A relevant example is Musashi1 (Msi1), which is emerging as a critical regulator of tumorigenesis in multiple cancer types, including colon cancer. Msi1 is a stem marker in several tissues and is critical in maintaining the balance between self-renewal and differentiation. However, a boost in Msi1 expression can most likely lead cells towards an oncogenic pathway. In this article, we discuss the parallels between Msi1 function in normal renewal of intestinal epithelium and in colon cancer.

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Section: Musashi1 In the Intestinal Epitheliummentioning

confidence: 97%

Section: Musashi1 In the Intestinal Epitheliummentioning

confidence: 99%

The RNA-Binding Protein Musashi1: A Major Player in Intestinal Epithelium Renewal and Colon Cancer Development

Plateroti

Araújo

Silva

et al. 2012

Curr Colorectal Cancer Rep

View full text Add to dashboard Cite

show abstract

“…Musashi1 (Msi1) is an RNA-binding protein that was initially identified in Drosophila melanogaster by its ability to regulate sensory organ development and asymmetric cell division [ 7 , 8 ]. In mammalian cells, Msi1 regulates translation in stem and progenitor cell populations of several tissues [ 8 - 10 ].…”

Section: Introductionmentioning

confidence: 99%

Musashi1 as a potential therapeutic target and diagnostic marker for lung cancer

Wang

Linnoila

et al. 2013

Oncotarget

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Lung cancer remains one of the leading causes of cancer-related deaths worldwide with a 5-year survival rate of less than 20%. One approach to improving survival is the identification of biomarkers to detect early stage disease. In this study, we investigated the potential of the stem cell and progenitor cell marker, Musashi1 (Msi1), as a diagnostic marker and potential therapeutic target for lung cancer. Functional studies in A549 bronchioalveolar carcinoma and NCI-H520 squamous cell carcinoma cells revealed that Msi1 was enriched in spheroid cultures of tumor cells and in the CD133+ cell population. Downregulation of Msi1 by lentivirus-mediated expression of an Msi1 shRNA reduced spheroid colony proliferation. Growth inhibition was associated with reduced nuclear localization of β-catenin and inhibition of the processing of intracellular Notch. In primary lung cancer, Msi1 protein expression was elevated in 86% of 202 tissue microarray specimens, and Msi1 mRNA was increased in 80% of 118 bronchoscopic biopsies, including metastatic disease, but was rarely detected in adjacent normal lung tissue and in non-malignant diseased tissue. Msi1 was expressed in a diffuse pattern in most tumor subtypes, except in squamous cell carcinomas, where it appeared in a focal pattern in 50% of specimens. Thus, Msi1 is a sensitive and specific diagnostic marker for all lung cancer subtypes.

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Musashi1 expression cells derived from mouse embryonic stem cells can be enriched in side population isolated by fluorescence activated cell sorter

Cited by 2 publications

References 56 publications

The RNA-Binding Protein Musashi1: A Major Player in Intestinal Epithelium Renewal and Colon Cancer Development

The RNA-Binding Protein Musashi1: A Major Player in Intestinal Epithelium Renewal and Colon Cancer Development

Musashi1 as a potential therapeutic target and diagnostic marker for lung cancer

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