Muscle Fibre Phenotyping from a Single Section: Is It as Informative as from Serial Sections?

Meznarič, Marija; Eržen, Ida

doi:10.5772/47845

Cited by 1 publication

(2 citation statements)

References 21 publications

(36 reference statements)

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“…On muscle sections fibers not immunoreactive with anti-slow MyHC-1 antibodies were all stained either heavily or intermediately with A4.74 antibodies which is in agreement that this antibody can be considered as an anti-fast myosin in humans (Figure 1) [7, 11]. …”

Section: Discussionsupporting

confidence: 70%

“…In human muscles 2A fibers are heavily stained with antibodies directed against MyHC-2A (A4.74 antibody) and 2X fibers stain intermediately [7, 11] which is similar to immunoreactivity of this antibody in dogs [12] but different from rats [7] in which this antibody stains only 2A fibers. On muscle sections fibers not immunoreactive with anti-slow MyHC-1 antibodies were all stained either heavily or intermediately with A4.74 antibodies which is in agreement that this antibody can be considered as an anti-fast myosin in humans (Figure 1) [7, 11].…”

Section: Discussionmentioning

confidence: 99%

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Size and Proportions of Slow-Twitch and Fast-Twitch Muscle Fibers in Human Costal Diaphragm

Meznarič

Cvetko

2016

BioMed Research International

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Smaller diaphragmatic motor unit potentials (MUPs) compared to MUPs of limb muscles lead to the hypothesis that diaphragmatic muscle fibers, being the generators of MUPs, might be also smaller. We compared autopsy samples of costal diaphragm and vastus lateralis of healthy men with respect to fibers' size and expression of slow myosin heavy chain isoform (MyHC-1) and fast 2A isoform (MyHC-2A). Diaphragmatic fibers were smaller than fibers in vastus lateralis with regard to the mean minimal fiber diameter of slow-twitch (46.8 versus 72.2 μm, p < 0.001), fast-twitch (45.1 versus 62.4 μm, p < 0.001), and hybrid fibers (47.3 versus 65.0 μm, p < 0.01) as well as to the mean fiber cross-sectional areas of slow-twitch (2376.0 versus 5455.9 μm2, p < 0.001), fast-twitch (2258.7 versus 4189.7 μm2, p < 0.001), and hybrid fibers (2404.4 versus 4776.3 μm2, p < 0.01). The numerical proportion of slow-twitch fibers was higher (50.2 versus 36.3%, p < 0.01) in costal diaphragm and the numerical proportion of fast-twitch fibers (47.2 versus 58.7%, p < 0.01) was lower. The numerical proportion of hybrid fibers did not differ. Muscle fibers of costal diaphragm have specific characteristics which support increased resistance of diaphragm to fatigue.

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Section: Discussionsupporting

confidence: 70%