Musculoskeletal structure and function in response to the combined effect of an obesogenic diet and age in male C57BL/6J mice

Bott, Kirsten N.; Gittings, William; Fajardo, Val A.; Baranowski, Bradley J.; Vandenboom, Rene; LeBlanc, Paul J.; Ward, Wendy E.; Peters, Sandra J.

doi:10.1002/mnfr.201700137

Cited by 15 publications

(32 citation statements)

References 32 publications

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“…Harlan Teklad Diet assisted in developing diets that were consistent with previous high fat studies (as previously outlined in detail by (Bott et al. )). Briefly, the control diet (AGE) consisted of 13.7% kcal protein, 75.9% kcal carbohydrates, 10.3% fat and contained 3.6 kcal/g energy.…”

Section: Methodsmentioning

confidence: 89%

“…The AGE (n = 12, diet TD.94048, Harlan Teklad, Mississauga, ON) and the AGE-HFS groups (AGE-HFS, n = 12, diet TD.150448, 45% kcal fat, Harlan Teklad, Mississauga, ON) were switched to their corresponding diets for the 13-week diet and aging intervention. Harlan Teklad Diet assisted in developing diets that were consistent with previous high fat studies (as previously outlined in detail by (Bott et al 2017)). Briefly, the control diet (AGE) consisted of 13.7% kcal protein, 75.9% kcal carbohydrates, 10.3% fat and contained 3.6 kcal/g energy.…”

Section: Experimental Designmentioning

confidence: 94%

“…Animals and the experimental design are those from a recent study published from Brock University (Bott et al. ). Male C57BL/6J mice (19‐weeks; 26.9 ± 1.9 g, n = 35) were purchased from The Jackson Laboratory (Bar Harbor, Maine, USA) and allowed to acclimatize for 5 days in the Brock University Comparative Biosciences Facility.…”

Section: Methodsmentioning

confidence: 99%

“…Experimental protocols were approved by the Brock University Animal Care Committee (file #15-06-01) and are in compliance with the Canadian Council on Animal Care. Animals and the experimental design are those from a recent study published from Brock University (Bott et al 2017). Male C57BL/6J mice (19-weeks; 26.9 AE 1.9 g, n = 35) were purchased from The Jackson Laboratory (Bar Harbor, Maine, USA) and allowed to acclimatize for 5 days in the Brock University Comparative Biosciences Facility.…”

Section: Animalsmentioning

confidence: 99%

“…The HFS diet consisted of 13.8% kcal protein, 40.8% kcal carbohydrates, 45.3% fat and contained 4.6 kcal/g energy. To account for the higher energy per quantity of HFS diet, protein and micronutrients were adjusted accordingly, to provide a similar level of these nutrients on the basis of energy (Buettner et al 2007;Bott et al 2017). These diet were phytoestrogen free.…”

Section: Experimental Designmentioning

confidence: 99%

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Evaluation of neuropathological effects of a high-fat high-sucrose diet in middle-aged male C57BL6/J mice

2018

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Metabolic dysfunction related to diet‐induced obesity has recently been linked to the pathogenesis of sporadic Alzheimer's disease (AD). However, the underlying mechanisms linking obesity and AD remain unclear. The purpose of this study was to examine early alterations in brain insulin signaling, inflammatory/stress markers, and energetic stress in a model of diet‐induced obesity during middle age. Male C57BL/6J mice were randomized to either a control diet (AGE n = 12) or high‐fat and sucrose diet (AGE‐HFS n = 12) for 13‐weeks from 20‐weeks of age. Prefrontal cortex and hippocampal samples were collected at 20‐weeks of age (BSL n = 11) and at 33‐weeks of age (AGE and AGE‐HFS). The HFS diet resulted in increased body weight (30%; P = 0.0001), increased %fat mass (28%; P = 0.0001), and decreased %lean mass (33%; P = 0.0001) compared to aged controls. In the prefrontal cortex, AGE‐HFS resulted in increased 5′ adenosine monophosphate – activated protein kinase (AMPK) phosphorylation (P = 0.045). In the hippocampus, AGE‐HFS resulted in increased extracellular signal‐regulated kinase (ERK) and c‐Jun N‐terminal kinase (JNK) phosphorylation and protein kinase B (Akt) serine473 and glycogen synthase kinase (GSK) phosphorylation (P < 0.05). Results from this study demonstrate that aging combined with a HFS diet results in increased inflammation (pERK and pJNK) and energetic stress (pAMPK) in the hippocampus and prefrontal cortex, respectively. Together these novel results provide important information for future targets in early AD pathogenesis.

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Section: Methodsmentioning

confidence: 89%

Section: Experimental Designmentioning

confidence: 94%

Section: Methodsmentioning

confidence: 99%

Section: Animalsmentioning

confidence: 99%

Section: Experimental Designmentioning

confidence: 99%

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Evaluation of neuropathological effects of a high-fat high-sucrose diet in middle-aged male C57BL6/J mice

2018

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Mouse models of sarcopenia: classification and evaluation

Xie

Dong-sheng

et al. 2021

J cachexia sarcopenia muscle

132

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Sarcopenia is a progressive and widespread skeletal muscle disease that is related to an increased possibility of adverse consequences such as falls, fractures, physical disabilities and death, and its risk increases with age. With the deepening of the understanding of sarcopenia, the disease has become a major clinical disease of the elderly and a key challenge of healthy ageing. However, the exact molecular mechanism of this disease is still unclear, and the selection of treatment strategies and the evaluation of its effect are not the same. Most importantly, the early symptoms of this disease are not obvious and are easy to ignore. In addition, the clinical manifestations of each patient are not exactly the same, which makes it difficult to effectively study the progression of sarcopenia. Therefore, it is necessary to develop and use animal models to understand the pathophysiology of sarcopenia and develop therapeutic strategies. This paper reviews the mouse models that can be used in the study of sarcopenia, including ageing models, genetically engineered models, hindlimb suspension models, chemical induction models, denervation models, and immobilization models; analyses their advantages and disadvantages and application scope; and finally summarizes the evaluation of sarcopenia in mouse models.

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Investigating a dose–response relationship between high-fat diet consumption and the contractile performance of isolated mouse soleus, EDL and diaphragm muscles

et al. 2018

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Musculoskeletal structure and function in response to the combined effect of an obesogenic diet and age in male C57BL/6J mice

Abstract: Consumption of a 13-week HFS diet at 20 weeks of age did not exacerbate age-related declines in bone or muscle, but these tissues do not decline in a coordinate manner with greater declines in bone than muscle.

Cited by 15 publications

References 32 publications

Evaluation of neuropathological effects of a high-fat high-sucrose diet in middle-aged male C57BL6/J mice

Evaluation of neuropathological effects of a high-fat high-sucrose diet in middle-aged male C57BL6/J mice

Mouse models of sarcopenia: classification and evaluation

Investigating a dose–response relationship between high-fat diet consumption and the contractile performance of isolated mouse soleus, EDL and diaphragm muscles

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