Mutagenicity studies on paracetamol in human volunteers II. Unscheduled DNA synthesis and micronucleus test

Topinka, Jan; Šrám, Radìm J.; Širinjan, G.; Kocisová, J; Binková, Blanka; Fojtíková, I.

doi:10.1016/0165-7992(89)90038-9

Cited by 29 publications

(4 citation statements)

References 14 publications

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“…In fact, several studies have failed to show any effect of smoking. One study has even reported no differences in the induction of micronuclei between smokers and nonsmokers, 25 and others have shown that smokers had less DNA damage than nonsmokers. 26 On the other hand, some authors have postulated increased DNA damage in heavy smokers.…”

Section: Discussionmentioning

confidence: 96%

Cytogenetic biomonitoring of oral mucosa cells from adults exposed to dental X-rays

Ribeiro

Angelieri

2008

Radiat Med

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Section: Discussionmentioning

confidence: 96%

Cytogenetic biomonitoring of oral mucosa cells from adults exposed to dental X-rays

Ribeiro

Angelieri

2008

Radiat Med

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“…This assay has been used to evaluate the exposure levels of the human population to mutagenic or genotoxic agents [26-30] as well as in cell cultures to determine the mutagenic potential of drugs and/or natural compounds [31-33]. …”

Section: Introductionmentioning

confidence: 99%

Cinnamic acid induces apoptotic cell death and cytoskeleton disruption in human melanoma cells

Niero

Machado-Santelli

2013

J Exp Clin Cancer Res

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Anticancer activities of cinnamic acid derivatives include induction of apoptosis by irreversible DNA damage leading to cell death. The present work aimed to compare the cytotoxic and genotoxic potential of cinnamic acid in human melanoma cell line (HT-144) and human melanocyte cell line derived from blue nevus (NGM). Viability assay showed that the IC50 for HT-144 cells was 2.4 mM, while NGM cells were more resistant to the treatment. The growth inhibition was probably associated with DNA damage leading to DNA synthesis inhibition, as shown by BrdU incorporation assay, induction of nuclear aberrations and then apoptosis. The frequency of cell death caused by cinnamic acid was higher in HT-144 cells. Activated-caspase 3 staining showed apoptosis after 24 hours of treatment with cinnamic acid 3.2 mM in HT-144 cells, but not in NGM. We observed microtubules disorganization after cinnamic acid exposure, but this event and cell death seem to be independent according to M30 and tubulin labeling. The frequency of micronucleated HT-144 cells was higher after treatment with cinnamic acid (0.4 and 3.2 mM) when compared to the controls. Cinnamic acid 3.2 mM also increased the frequency of micronucleated NGM cells indicating genotoxic activity of the compound, but the effects were milder. Binucleation and multinucleation counting showed similar results. We conclude that cinnamic acid has effective antiproliferative activity against melanoma cells. However, the increased frequency of micronucleation in NGM cells warrants the possibility of genotoxicity and needs further investigation.

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“…Tobacco is known to contain various genotoxic chemical, and smoking is a well-documented cause of cancer including the oral cavity 32 . For this reason, smokers and non-smokers are investigated comparatively as smoking is supposed to be relevant to genotoxin exposure 33 . Our results appeared to demonstrate no effect in micronuclei frequency between smokers and non-smokers, either to patients or to control groups.…”

Section: Discussionmentioning

confidence: 99%

Cytogenetical damage in exfoliated oral mucosa cells in elderly people suffering denture stomatitis

Matsumoto¹,

Castanho²,

Kawakami³

et al. 2010

Gerodontology

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Mutagenicity studies on paracetamol in human volunteers II. Unscheduled DNA synthesis and micronucleus test

Cited by 29 publications

References 14 publications

Cytogenetic biomonitoring of oral mucosa cells from adults exposed to dental X-rays

Cytogenetic biomonitoring of oral mucosa cells from adults exposed to dental X-rays

Cinnamic acid induces apoptotic cell death and cytoskeleton disruption in human melanoma cells

Cytogenetical damage in exfoliated oral mucosa cells in elderly people suffering denture stomatitis

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