Mutation Breeding in Vegetatively Propagated Floricultural Crops

Broertjes, C.

doi:10.17660/actahortic.1976.63.23

Cited by 23 publications

(43 citation statements)

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“…This variation which occurs naturally or artificially induced by physical, biological or chemical mutagens has attracted the interest of plant breeders for many decades. Mutations had enhanced the production of improved cultivars with economic value (Broertjes and van Harten, 1988) as well as the study of genetics and plant developmental phenomena (Bertagne-Sagnard et al, 1996;Wongpiyasatid et al, 2000). Mutation breeding is an aspect of conventional plant breeding which involves physical and chemical mutation in order to assess genetic variation resulting to improved varieties with better characteristics (Wongpiyasatid et al, 2000;Mensah and Obadoni, 2007;Arulbalachandran et al, 2009;Olawuyi et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Genetic Variability on Tolerance of Maize (<i>Zea mays</i> L.) Genotypes Induced with Sodium Azide Mutagen

Olawuyi¹,

Okoli²

2017

mpb

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variability on tolerance of maize (Zea mays L.) genotypes induced with sodium azide mutagen, Molecular Plant Breeding, 8(3): 27-37 (doi: 10.5376/mpb.2017.08.0003) Abstract This study investigated the mutagenic variability of maize (Zea mays L.) genotypes induced with sodium azide (NaN3).The seeds of maize genotypes; TZE-WDTSTR C4, TZM 154, SAMMAZ 15, DTE-YSR BC3, EV99 QPM, DTSR COF2, TZM 146 and TZM 1441 were pre-soaked in distilled water for six hours before treated with 20ml of sodium azide at 35, 45, 55, and 65 mM for six hours. The untreated maize served as control. The seeds were sown on perforated polythene bags containing 7kg of soil with three replicates in a complete randomized design. The observations from seedling to maturity recorded albino, xantha and viridis mutant traits. The mitotic studies carried out on the root tips of the seedlings revealed chromosomal aberrations such as abnormal anaphase and metaphase and chromosomal deletion. There were variations among the genotypes for yield, morpho-agronomic traits and mutation tolerance. TZE-WDTSTR C4, TZM 154 and TZM 146 were early maturing genotypes. TZM 1441 and EV99 QPM were the most tolerant genotypes to mutagenic effect, while EV99 QPM performed best for percentage seed germination, ear aspect, husk cover and mutation tolerance. The mutation tolerance was positive and strongly correlated with agronomic and yield traits (days to first tasseling, tasseling length, plant stand, ear aspect, husk cover and number of cobs) compared to growth traits. The number of cobs produced is positive and strongly related with mutation tolerance at r = 0.77; p < 0.01, while the ear aspect is positive and strongly associated with husk cover, number of cobs and mutation tolerance at r = 0.86, 0.76 and 0.79 respectively. The first Principal Component Axis (Prin. 1) had the highest contribution to the variation of morphological, yield and mutation tolerance traits with proportion and Eigen value of 23.12% and 3.24 respectively, while Prin. 14 had the least contribution. Stem height, grain weight, shoot biomass and root biomass were closely related to mutation tolerance in Prin. 1 than other traits. Therefore, selection of the most tolerant genotypes to sodium azide (chemical mutagen) should be considered in breeding programmes.

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Section: Introductionmentioning

confidence: 99%

Genetic Variability on Tolerance of Maize (<i>Zea mays</i> L.) Genotypes Induced with Sodium Azide Mutagen

Olawuyi¹,

Okoli²

2017

mpb

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“…A matter of interest for plant breeders is the use of mutagens, in combination with in vitro cultures, to create genetic variation (Broertjes and Van Harten, 1988). Determination of the radiosensitivity of in vitro buds to gamma rays is the initial step since it focuses on early screening of variants with altered genetic patterns.…”

Section: Effect Of Gamma Rays On Shoot Regeneration Of In Vitro Budsmentioning

confidence: 99%

“…Plant breeders combine tissue culture technique for rapid multiplication of regenerants, mutation induction to enhance variations, and molecular marker methods to detect the genetic variation (Ahloowalia and Maluszynski, 2001). Such a combination of techniques has been exploited for the creation of new and novel plant cultivars, particularly in vegetatively propagated species (Broertjes and Van Harten, 1988;Pinet-Leblay et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

In vitro mutagenesis of Etlingera elatior (Jack) and early detection of mutation using RAPD markers

Yunus¹,

Aziz²,

Kadir³

et al. 2013

Turk J Biol

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Mutation breeding techniques in combination with tissue culture and molecular marker methods provide a powerful tool for improvement of vegetatively propagated plants. The aim of this study was to develop a protocol for shoot regeneration and mutation induction of Etlingera elatior. The results of irradiation on in vitro buds of E. elatior showed LD 50 to be 10 Gy, with the survival of explants being sharply reduced at this dosage. All 8 selected gamma irradiated regenerants were differentiated from the untreated control based on the banding patterns obtained using 9 primers, which generated 59 reproducible bands, whereby 35 (55.31%) were found to be polymorphic. Jaccard's coefficient of similarity values ranging from 0.537 to 0.860 were indicative of the level of genetic variation among the mutants studied. For comparison between the potential lines (PL) and the control, a maximum similarity value (0.814) was observed in PL1 mutant, while the minimum value (0.537) was observed in PL7. In summary, a combination of irradiation, regeneration, multiplication, and random amplification of polymorphic DNA (RAPD) analysis for early screening of mutants can speed up the breeding program of E. elatior.

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“…Segundo Broertjes & Van Harten (1988), a explicação para o aparecimento de altas porcentagens de plantas não quiméricas, a partir daquele explante, seria de que a nova brotação teria origem unicelular. No presente trabalho não se determinou se as brotações tiveram origem unicelular ou multicelular.…”

Section: Radiossensibilidade Em Cotilédones De Embriões Nucelaresunclassified

“…No presente trabalho não se determinou se as brotações tiveram origem unicelular ou multicelular. Entretanto, existem várias formas de evitar a formação de quimeras, mesmo nas situações em que as gemas se formam a partir de mais de uma célula (Broertjes & Van Harten, 1988). Venverloo et ai.…”

Section: Radiossensibilidade Em Cotilédones De Embriões Nucelaresunclassified

Uso de mutagênese "in vitro" no melhoramento de citros: I. Sensibilidade a raios gama de explantes do cultivar pêra

et al. 1994

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Para futuros trabalhos com indução de mutação "in vitro", determinou-se a sensibilidade de diferentes explantes do cultivar de laranja Pêra (Citrus sinensis Osbeck) a raios gama visando a escolha das doses a utilizar. Para isso, após a irradiação, avaliou-se o número de explantes responsivos, de embrióides e de brotações adventícias, a massa do calo e a formação de enraizamento. Resultados diferentes foram obtidos de acordo com o tecido empregado. Assim, para a irradiação de nucelos, recomenda-se o uso de doses entre 20 e 40 Gy; em calos embriogênicos, de 120 Gy e, para a irradiação de cotilédones de embriões nucelares, a dose situa-se ao redor de 40 Gy.

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Mutation Breeding in Vegetatively Propagated Floricultural Crops

Cited by 23 publications

References 0 publications

Genetic Variability on Tolerance of Maize (<i>Zea mays</i> L.) Genotypes Induced with Sodium Azide Mutagen

Genetic Variability on Tolerance of Maize (<i>Zea mays</i> L.) Genotypes Induced with Sodium Azide Mutagen

In vitro mutagenesis of Etlingera elatior (Jack) and early detection of mutation using RAPD markers

Uso de mutagênese "in vitro" no melhoramento de citros: I. Sensibilidade a raios gama de explantes do cultivar pêra

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