Mutation ofp53Is Not a Prerequisite for Immortalization of Human Fibroblasts by SV40 T Antigen

Moorwood, Kim; Price, T. N. C.; Mayne, Lynne

doi:10.1006/excr.1996.0086

Cited by 14 publications

(7 citation statements)

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“…As indicated in the previous paragraphs, tissue culture experiments indicated that usually the continuous expression of Tag is required for the maintenance of the transformed phenotype (reviewed in Ozer et al, 1996). However, as discussed, recent results suggest that in human cells in tissue culture (Moorwood, 1996), and in tumors developing in SV40-transgenic mice (Maroulokau et al, 1994;Ewald et al, 1996) transient expression of Tag is su cient to induce transformation. Therefore, while we would expect that most tumor cells should express Tag, it cannot be excluded that some do not.…”

Section: Technical Concerns and Working Hypothesis To Determine The Omentioning

confidence: 99%

Simian virus 40, poliovaccines and human tumors: a review of recent developments

1997

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Recently, wild-type SV40 and/or DNA sequences indistinguishable from SV40 have been detected in speci®c types of human tumors: ependymoma and choroid plexus tumors, mesothelioma, osteosarcoma and sarcoma. The same tumor types will develop in hamsters after injection with SV40. These ®ndings are interesting in themselves for they could shed light on the pathogenesis of these tumors. These ®ndings also have public health implications. SV40 was found to have contaminated the poliovaccines and the adenovaccines from 1955 until 1963, therefore resulting in the inadvertent injection of millions of people with this tumor virus. Moreover, our society pays a high cost for asbestos causality, a carcinogen associated with the development of mesothelioma. In addition to asbestos, the potential impact of ®nding another possible cause for mesothelioma (i.e., SV40), as well as the possible pathogenic role of the contaminated poliovaccines, has generated considerable public interest and concern. To discuss these recent ®ndings, the NIH (National Institutes of Health) and the FDA (Food and Drug Administration), organized an International Conference at the NIH, Bethesda, MD, January 27 ± 28, 1997. The association of SV40 with human mesothelioma was also discussed in a special session at the IV International Mesothelioma Conference that was held at the University of Pennsylvania, Philadelphia, PA, May 13 ± 16, 1997. The purpose of this review is to summarize data, from the discovery of the contaminated poliovaccines, to the most recent ®ndings presented at the meetings in Bethesda and Philadelphia, to discuss technical and other problems associated with this research, and the potential for using these ®ndings to develop new diagnostic and therapeutic approaches for SV40-associated malignancies.

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Section: Technical Concerns and Working Hypothesis To Determine The Omentioning

confidence: 99%

Simian virus 40, poliovaccines and human tumors: a review of recent developments

1997

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“…There are multiple lines of evidence, in transgenic mice (Ewald et al, 1996;Tzeng et al, 1998), in rat glioblastoma (Salewski et al, 1999) and in human cells in vitro (Moorwood et al, 1996), indicating that SV40 can transform with a 'hit and run' type of mechanism.…”

Section: Molecular Mechanisms Of Sv40 Carcinogenesismentioning

confidence: 99%

New developments about the association of SV40 with human mesothelioma

et al. 2003

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“…Mutations and deletions of the SV40 genome may also occur in human tumours but are very unlikely to occur in the Rb pocket binding domain of tantigen because of the relevance of this region in cell transformation [23,24]. It is also possible that the sequence detected by these sets of primers belong to a recombinant between SV40 and another unknown virus.…”

Section: Discussionmentioning

confidence: 99%

Detection of SV40 like viral DNA and viral antigens in malignant pleural mesothelioma

Ramael¹,

Nagels²,

Heylen³

et al. 1999

Eur Respir J

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This study investigated the presence of simian vacuolating virus 40 (SV40) deoxyribonucleic acid (DNA) in malignant pleural mesothelioma, non-neoplastic mesothelium and pleural carcinoma metastasis and correlated these data with immunohistochemistry for SV40 viral antigens.The novel Primed In Situ (PRINS) method was applied to detect the presence of SV40 DNA in situ in tissue sections of malignant mesothelioma (n=25), non-neoplastic mesothelium (n=30) and pleural carcinoma metastasis (n=30). Immunohistochemistry with an SV40-specific antibody was applied for detection of the SV40 viral antigen in the same material.SV40 DNA and expression of one of the viral proteins (small t-antigen) was found iñ 60% of the investigated mesothelioma cases in contrast to non-neoplastic mesothelium and carcinoma metastasis that were negative for both SV40 DNA and SV40 viral antigens.These results suggest that simian vacuolating virus 40 deoxyribonucleic acid may be biologically active as there was also immunoreactivity for simian vacuolating virus 40 viral antigen in those cases positive for simian vacuolating virus 40 deoxyribonucleic acid with the primed in situ reaction. Simian vacuolating virus 40 viral deoxyribonucleic acid and antigens may be potential markers for neoplastic mesothelium that may prove useful in the rather difficult histopathological differential diagnosis between malignant mesothelioma and reactive mesothelium or pleural carcinoma. Eur Respir J 1999; 14: 1381±1386.

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Mutation ofp53Is Not a Prerequisite for Immortalization of Human Fibroblasts by SV40 T Antigen

Cited by 14 publications

References 0 publications

Simian virus 40, poliovaccines and human tumors: a review of recent developments

Simian virus 40, poliovaccines and human tumors: a review of recent developments

New developments about the association of SV40 with human mesothelioma

Detection of SV40 like viral DNA and viral antigens in malignant pleural mesothelioma

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